CAJ | 학술논문

目的：研究psbA-trnH序列在近缘贯众基原物种鉴定中的有效性。方法：对常见鳞毛蕨属贯众基原物种psbA-trnH序列进行PCR扩增及测序，原始峰图通过CodonCodeAligner软件查看、拼接和校对，并根据GenBank注释截取标准条形码数据。利用TaxonGap方法在实验物种范围（9物种19样品）和扩大范围（17物种44样品）内评估该序列的鉴定效率。结果：物种鉴定范围扩大后psbA-trnH序列鉴定成功率由100%（9/9）降至82.4%（14/17），种内异质性数值高于种间可分离性的比例，由11.1%（1/9）上升为52.9%（9/17）。结论：psbA-trnH序列可作为整体贯众同名异物品种鉴定的推荐条形码，实际应用中需注意种内采样代表性的问题。
목적：연구psbA-trnH서렬재근연관음기원물충감정중적유효성。방법：대상견린모궐속관음기원물충psbA-trnH서렬진행PCR확증급측서，원시봉도통과CodonCodeAligner연건사간、병접화교대，병근거GenBank주석절취표준조형마수거。이용TaxonGap방법재실험물충범위（9물충19양품）화확대범위（17물충44양품）내평고해서렬적감정효솔。결과：물충감정범위확대후psbA-trnH서렬감정성공솔유100%（9/9）강지82.4%（14/17），충내이질성수치고우충간가분리성적비례，유11.1%（1/9）상승위52.9%（9/17）。결론：psbA-trnH서렬가작위정체관음동명이물품충감정적추천조형마，실제응용중수주의충내채양대표성적문제。
This article was aimed to study the species identification capability of psbA-trnH among Guanzhong herbs from Dryopteri. The nucleotide sequence information of psbA-trnH region was abstracted using standardized manners from 9 Dryopteri species (19 samples). The identification efficiency of psbA-trnH was analyzed based on TaxonGap among both tested materials (9 species, 19 samples) and tested materials plus GenBank data (17 species, 44 samples in total). The results showed that with the expanded species range, the discriminative efficiency of psbA-trnH de-clined from 100% (9/9) to 82.4% (14/17), while the proportion of within-specific heterogeneity larger than between-specific separability increased from 11.1% (1/9) to 52.9% (9/17). It was concluded that psbA-trnH can be recom-mended as the valuable barcode for homonym distinguishment among Guanzhong herbs, with attention of adequate sampling within species.