中国医院用药评价与分析
中國醫院用藥評價與分析
중국의원용약평개여분석
EVALUATION AND ANAL YSIS OF DRUG-USE IN HOSPITALS OF CHINA
2014年
8期
695-697
,共3页
槲皮素%黄酮类化合物%荧光分光光度法
槲皮素%黃酮類化閤物%熒光分光光度法
곡피소%황동류화합물%형광분광광도법
Quercetin%Flavonoids%Spectrofluorimetry
目的：建立一种新的测定大山楂丸中黄酮类化合物的荧光分光光度法。方法：以槲皮素为对照品，设定荧光激发/发射波长为281/352 nm，激发和发射光狭缝宽度为5 nm，在300～500 nm波长范围内测定荧光光谱。对酸度、表面活性剂、温度、反应时间及铝离子浓度等影响因素进行考察，优化试验条件。结果：在盐酸酸性介质中、无表面活性剂、常温下、反应时间30 min、铝离子浓度为2×10-4 mol/L时，体系的荧光强度最大且稳定。当槲皮素的浓度范围在0.2×10-5～1.5×10-5 mol/L之间时，荧光强度（F）和槲皮素浓度（c）呈良好的线性关系；检测限为2.4×10-8 mol/L。3批大山楂丸中总黄酮的平均含量分别为24.56、27.85、25.13 mg/g，与《中国药典》方法测定结果基本一致。结论：本方法操作简便、快速、准确，可用于测定大山楂丸中总黄酮的含量。
目的：建立一種新的測定大山楂汍中黃酮類化閤物的熒光分光光度法。方法：以槲皮素為對照品，設定熒光激髮/髮射波長為281/352 nm，激髮和髮射光狹縫寬度為5 nm，在300～500 nm波長範圍內測定熒光光譜。對痠度、錶麵活性劑、溫度、反應時間及鋁離子濃度等影響因素進行攷察，優化試驗條件。結果：在鹽痠痠性介質中、無錶麵活性劑、常溫下、反應時間30 min、鋁離子濃度為2×10-4 mol/L時，體繫的熒光彊度最大且穩定。噹槲皮素的濃度範圍在0.2×10-5～1.5×10-5 mol/L之間時，熒光彊度（F）和槲皮素濃度（c）呈良好的線性關繫；檢測限為2.4×10-8 mol/L。3批大山楂汍中總黃酮的平均含量分彆為24.56、27.85、25.13 mg/g，與《中國藥典》方法測定結果基本一緻。結論：本方法操作簡便、快速、準確，可用于測定大山楂汍中總黃酮的含量。
목적：건립일충신적측정대산사환중황동류화합물적형광분광광도법。방법：이곡피소위대조품，설정형광격발/발사파장위281/352 nm，격발화발사광협봉관도위5 nm，재300～500 nm파장범위내측정형광광보。대산도、표면활성제、온도、반응시간급려리자농도등영향인소진행고찰，우화시험조건。결과：재염산산성개질중、무표면활성제、상온하、반응시간30 min、려리자농도위2×10-4 mol/L시，체계적형광강도최대차은정。당곡피소적농도범위재0.2×10-5～1.5×10-5 mol/L지간시，형광강도（F）화곡피소농도（c）정량호적선성관계；검측한위2.4×10-8 mol/L。3비대산사환중총황동적평균함량분별위24.56、27.85、25.13 mg/g，여《중국약전》방법측정결과기본일치。결론：본방법조작간편、쾌속、준학，가용우측정대산사환중총황동적함량。
OBJECTIVE:To determine the content of total flavonoids in Crataegi Bolus by spectrofluorimetry. METHODS:The content of the total flavonoids in Crataegi Bolus was determined by spectrofluorimetry with quercetin as reference substance with wavelength of fluorescence excitation/emission of 281/352 nm,slit width of excitation/emission of 5 nm and the fluorescence spectra of 300-500 nm. The influential factors such as acidity,surfactant,temperature,reaction time and the concentration of alumi-num ion were investigated to optimize the experimental conditions. RESULTS:The maximum and stable fluorescence intensity was obtained in acid medium of hydrochloric acid in the absence of surfactant under room temperature with reaction time of 30 min and aluminum ion concentration of 2×10-4 mol/L. Fluorescence intensity(F)and the quercetin concentration(c)were in good linear re-lationship when the concentration of quercetin was between 0.2 × 10-5 and 1.5 × 10-5 mol/L. The detection limit of this method was 2.4 × 10-8 mol/L. The average contents of the total flavonoids in three batches of Crataegi Bolus were 25.45 mg/g,27.85 mg/g and 25.13 mg/g,respectively,which were basically in conformity with those determined by the method specified in China Phamacopoe-ia. CONCLUSIONS:The method is simple,rapid and accurate and thus it is applicable for determination of the total flavonoids in Crataegi Bolus.
