CAJ | 학술논문

目的：通过检测麻风病患者血清IL-10及外周血CD4＋CD25＋Foxp3＋调节性T细胞（ Treg）的水平，分析它们相互间的关系，探讨IL-10及调节性T细胞引起麻风患者免疫功能异常的机制。方法：用流式细胞微球芯片捕获技术（ CBA）检测30例麻风患者及25例正常对照血清IL-10的浓度；用流式细胞仪检测51例麻风患者及56例正常对照外周血Treg细胞的水平。结果：麻风患者血清IL-10浓度（130.00±54.27）pg/ml比对照组（15.64±7.11） pg/ml高（P＜0.05）；外周血Treg细胞水平（17.626±8.1977）％比对照组（9.998±1.7062）％高（P＜0.05）；麻风患者治愈后血清IL-10的浓度与外周血Treg细胞水平呈正相关线性关系（ P＜0.05）。结论：IL-10及Treg细胞的调控失衡可能是引起麻风发病的原因之一。
목적：통과검측마풍병환자혈청IL-10급외주혈CD4＋CD25＋Foxp3＋조절성T세포（ Treg）적수평，분석타문상호간적관계，탐토IL-10급조절성T세포인기마풍환자면역공능이상적궤제。방법：용류식세포미구심편포획기술（ CBA）검측30례마풍환자급25례정상대조혈청IL-10적농도；용류식세포의검측51례마풍환자급56례정상대조외주혈Treg세포적수평。결과：마풍환자혈청IL-10농도（130.00±54.27）pg/ml비대조조（15.64±7.11） pg/ml고（P＜0.05）；외주혈Treg세포수평（17.626±8.1977）％비대조조（9.998±1.7062）％고（P＜0.05）；마풍환자치유후혈청IL-10적농도여외주혈Treg세포수평정정상관선성관계（ P＜0.05）。결론：IL-10급Treg세포적조공실형가능시인기마풍발병적원인지일。
To detect the correlation between Interleukin-10 and CD4+CD25+Foxp3+regulation T cells ( Treg) and to explore the significance and mechanism of them in leprosy patients.Methods:We used the cytometric bead arra (CBA) to detect the serum Interleukin-10 in 30 leprosy patients and we used flow cytometry to test the Treg cells in 51 leprosy persons.Results:The con-centration of serum IL-10 in leprosy patients(130.00±54.27)pg/ml was higher than normal people(15.64±7.11)pg/ml,(P<0.05);The percentage of Treg cells in leprosy patients(17.626±8.1977)%was higher than normal people(9.998±1.7062)%,(P<0.05).The relevance between IL-10 and Treg is positive and linear ,( P<0.05 ).Conclusion: The imbalance between IL-10 and Treg cells may lead to immune abnormal in leprosy patients.