中国免疫学杂志
中國免疫學雜誌
중국면역학잡지
CHINESE JOURNAL OF IMMUNOLOGY
2014年
8期
1098-1100
,共3页
李玉香%汪杨%高玉伟%王峰
李玉香%汪楊%高玉偉%王峰
리옥향%왕양%고옥위%왕봉
甲型H1N1流感病毒%甲型H1N1特异性抗体%甲型H1N1流感病毒RNA
甲型H1N1流感病毒%甲型H1N1特異性抗體%甲型H1N1流感病毒RNA
갑형H1N1류감병독%갑형H1N1특이성항체%갑형H1N1류감병독RNA
H1N1 influenza A virus%Specific antibody to H1N1 influenza%RNA of H1N1 influenza A virus
目的:了解甲型H1 N1流感病毒的流行特点及传播规律,加强对流感大流行的防控。方法:收集2009年10~12月吉林大学第一医院住院发热门诊中具有流感症状患者的临床样品(鼻咽拭子和血清),采用血凝抑制法、巢式 RT-PCR技术检测具有流感症状患者血清标本中的特异性抗体、咽拭子中的甲型H1N1 RNA 等指标。结果:结果56份流感患者的咽拭子经核酸检测,甲型流感NP和M的阳性率分别为37.5%和27.8%,而甲型H1N1的阳性率仅为3.6%。通过血凝抑制实验结果表明:在相应的血清标本中,能特异性抑制甲型H1N1流感病毒红细胞凝集的样品为27份,抗体均大于1∶320,其中8份样品抗体效价大于1∶5120。恢复期的血清效价与急性期相比有显著的抗体差异,甲型H1 N1病毒特异性抗体阳性率为48.2%,明显高于核酸的检测。结论:核酸检测可在感染早期(排毒期)进行检测,而血清学的抗体检测多用在感染中后期(抗体产生)检测,两者联合可提高甲型H1 N1流感的检出率。
目的:瞭解甲型H1 N1流感病毒的流行特點及傳播規律,加彊對流感大流行的防控。方法:收集2009年10~12月吉林大學第一醫院住院髮熱門診中具有流感癥狀患者的臨床樣品(鼻嚥拭子和血清),採用血凝抑製法、巢式 RT-PCR技術檢測具有流感癥狀患者血清標本中的特異性抗體、嚥拭子中的甲型H1N1 RNA 等指標。結果:結果56份流感患者的嚥拭子經覈痠檢測,甲型流感NP和M的暘性率分彆為37.5%和27.8%,而甲型H1N1的暘性率僅為3.6%。通過血凝抑製實驗結果錶明:在相應的血清標本中,能特異性抑製甲型H1N1流感病毒紅細胞凝集的樣品為27份,抗體均大于1∶320,其中8份樣品抗體效價大于1∶5120。恢複期的血清效價與急性期相比有顯著的抗體差異,甲型H1 N1病毒特異性抗體暘性率為48.2%,明顯高于覈痠的檢測。結論:覈痠檢測可在感染早期(排毒期)進行檢測,而血清學的抗體檢測多用在感染中後期(抗體產生)檢測,兩者聯閤可提高甲型H1 N1流感的檢齣率。
목적:료해갑형H1 N1류감병독적류행특점급전파규률,가강대류감대류행적방공。방법:수집2009년10~12월길림대학제일의원주원발열문진중구유류감증상환자적림상양품(비인식자화혈청),채용혈응억제법、소식 RT-PCR기술검측구유류감증상환자혈청표본중적특이성항체、인식자중적갑형H1N1 RNA 등지표。결과:결과56빈류감환자적인식자경핵산검측,갑형류감NP화M적양성솔분별위37.5%화27.8%,이갑형H1N1적양성솔부위3.6%。통과혈응억제실험결과표명:재상응적혈청표본중,능특이성억제갑형H1N1류감병독홍세포응집적양품위27빈,항체균대우1∶320,기중8빈양품항체효개대우1∶5120。회복기적혈청효개여급성기상비유현저적항체차이,갑형H1 N1병독특이성항체양성솔위48.2%,명현고우핵산적검측。결론:핵산검측가재감염조기(배독기)진행검측,이혈청학적항체검측다용재감염중후기(항체산생)검측,량자연합가제고갑형H1 N1류감적검출솔。
H1N1 influenza A spread around the world in 2009.There were lots of patients in China too.We did this research to know the epidemiological feature and transmission route and strengthen the prevention and control measure of the influenza.Methods:We collected the nasopharyngeal swab and serum samples of 56 patients who had flu symptom from the infectious disease department of 1st hospital of Jilin University from October in 2009 to December in 2009.The specific antibody of the serum samples were detected by the blood clots suppression method and the H 1N1 RNA of the nasopharyngeal swab was detected by the Nest-RT-PCR assay.Results:The results of nucleic acid test showed that 21(37.5%) and 16(27.8%) samples were found NP and M of influenza A positive respectively and only 2 ( 3.6%) were found H1N1 of influenza A positive.The results of the blood clots suppression method showed that the serum samples of 27 patients (48.2%) could suppress the red blood cells blot of H 1N1 influenza A specifically and all the antibody titer was more than 1∶320.The antibody titer was more than 1∶5 120 in 8 of them.There′s significant difference of the serum antibody titer between the recovery phase and the acute phase.The specific H1N1 influenza A antibody of 27 (48.2%) serum samples in the recovery phase were positive and it was much higher than the result of nucleic acid test .Conclusion:The nucleic acid could be detected in the acute phase and the serum antibody detection could be done in the later stage .Using both the assays could increase the positive rate of H 1N1 influenza.