CAJ | 학술논문

目的：探讨珍宝丸预处理对新生大鼠缺氧性脑损伤Fas和Fas L蛋白表达的影响。方法：取新生7 d Wistar大鼠200只，雌雄不限，随机分成5组（n＝40）：正常对照组、模型缺氧1 h组（Ⅰm-12 h、Ⅰm-24 h、Ⅰm-48 h、Ⅰm-72 h）、模型缺氧4 h组（Ⅳm-12 h、Ⅳm-24 h、Ⅳm-48 h、Ⅳm-72 h ）、珍宝丸缺氧1 h治疗组（ⅠT-14 h、ⅠT-24 h、ⅠT-48 h、ⅠT-72 h ）、珍宝丸缺氧4 h治疗组（ⅣT-14 h、ⅣT-24 h、ⅣT-48 h、ⅣT-72 h）。将新生Wistar大鼠置于8％O2＋92％N2（V/V）的缺氧箱内建立新生鼠缺氧模型。在造模前每日一次连续7 d灌胃给予生理盐水和珍宝丸，缺氧期间也同样剂量给药，分别于缺氧后12、24、48、72 h处死动物取血及脑组织待测。采用ELISA法检测大鼠血清及脑组织悬液中Fas和Fas L的蛋白含量；采用免疫组织化学方法检测各组大鼠脑组织中Fas和Fas L蛋白的阳性表达。结果：与正常对照组比较，其他四组Fas和Fas L蛋白含量、蛋白的阳性表达均在缺氧后12 h时间点明显增高，且在24 h时间点达高峰，有统计学意义（ P＜0.01），模型组在各时间点上明显高于治疗组；模型组在48、72 h时间点Fas和Fas L表达低于12 h时间点，但仍明显高于正常对照组（ P＜0.01）；珍宝丸治疗组在48、72 h时间点Fas和Fas L表达低于12 h时间点，但仍高于正常对照组（ P＜0.05）。结论：珍宝丸可通过下调缺氧性脑损伤大鼠Fas和Fas L的蛋白含量、蛋白的阳性表达强度，从而对缺氧性脑损伤具有一定的保护作用。
목적：탐토진보환예처리대신생대서결양성뇌손상Fas화Fas L단백표체적영향。방법：취신생7 d Wistar대서200지，자웅불한，수궤분성5조（n＝40）：정상대조조、모형결양1 h조（Ⅰm-12 h、Ⅰm-24 h、Ⅰm-48 h、Ⅰm-72 h）、모형결양4 h조（Ⅳm-12 h、Ⅳm-24 h、Ⅳm-48 h、Ⅳm-72 h ）、진보환결양1 h치료조（ⅠT-14 h、ⅠT-24 h、ⅠT-48 h、ⅠT-72 h ）、진보환결양4 h치료조（ⅣT-14 h、ⅣT-24 h、ⅣT-48 h、ⅣT-72 h）。장신생Wistar대서치우8％O2＋92％N2（V/V）적결양상내건립신생서결양모형。재조모전매일일차련속7 d관위급여생리염수화진보환，결양기간야동양제량급약，분별우결양후12、24、48、72 h처사동물취혈급뇌조직대측。채용ELISA법검측대서혈청급뇌조직현액중Fas화Fas L적단백함량；채용면역조직화학방법검측각조대서뇌조직중Fas화Fas L단백적양성표체。결과：여정상대조조비교，기타사조Fas화Fas L단백함량、단백적양성표체균재결양후12 h시간점명현증고，차재24 h시간점체고봉，유통계학의의（ P＜0.01），모형조재각시간점상명현고우치료조；모형조재48、72 h시간점Fas화Fas L표체저우12 h시간점，단잉명현고우정상대조조（ P＜0.01）；진보환치료조재48、72 h시간점Fas화Fas L표체저우12 h시간점，단잉고우정상대조조（ P＜0.05）。결론：진보환가통과하조결양성뇌손상대서Fas화Fas L적단백함량、단백적양성표체강도，종이대결양성뇌손상구유일정적보호작용。
To investigate the effect of treasure pill pretreatment on hypoxic brain injury Fas and Fas L protein expression.Methods:Newborn 7d Wistar rats 200,male or female,were randomly divided into 5 groups(n=40):normal control group, model group 1h hypoxia(Ⅰm-12h,Ⅰm-24h,Ⅰm-48h,Ⅰm-72h),model group 4h hypoxia(Ⅳm-12h,Ⅳm-24h,Ⅳm-48h,Ⅳm-72h), hypoxic 1h treasure pill treatment group(ⅠT-14h,ⅠT-24h,ⅠT-48h,ⅠT-72h),hypoxic 4h treasure pill treatment group(ⅣT-14h,ⅣT-24 h ,ⅣT-48 h ,ⅣT-72 h ).The newborn Wistar rats were placed in hypoxia chamber of 8%O2+92%N2 ( V/V) for establishment the model of neonatal rats with hypoxia.Before modeling continuous 7 d once-daily oral administration of saline and treasure pill , during hypoxia also dose ,after hypoxia 12 h,24 h,48 h,72 h animals were sacrificed blood and brain tissues tested.By ELISA to detect the protein levels of Fas and Fas L in serum and brain tissue;by immunohistochemistry method to detect the expression of Fas and Fas L in rat brain tissue.Results:Compared with normal control group ,other four groups the protein content and protein expression of Fas and Fas L are in the 12h time points after hypoxic significantly increased ,and 24 h time point that pealed at statistically significant ( P<0.01 ) ,model group was significantly higher than the treatment group at each time point ;model groups in 48 h,72 h time point the protein expression and protein content of Fas and Fas L was less than 12 h time point but still significantly higher than the control group ( P<0.01 );treasure pill groups in 48 h,72 h time point the protein expression and protein content of Fas and Fas L was less than 12 h time point ,and slightly higher than the normal control group ( P<0.05 ).Conclusion: Treasures pill can cut hypoxic brain damage in rats,the positive expression of Fas and Fas L protein content and protein ,and thus the hypoxic brain injury has a protective effect.