CAJ | 학술논문

目的：研究黄芪多糖（ APS）对猪布鲁菌S2株感染小鼠免疫功能的调节作用。方法：120只BALB/c小鼠随机分为4组，实验组小鼠分别腹腔注射0.4、1.2、3 mg/ml的黄芪多糖1 ml，1次/d，对照组注射同体积的生理盐水，连续3 d。第4天腹腔注射1×107 L-1的猪布鲁菌S2株1 ml进行感染，分别于感染后1、6、12、24、48、72 h眼球放血后处死5只小鼠，取腹腔巨噬细胞（ Macrophage ，MΦ）涂片、瑞-姬氏染色，计算感染1 h后吞噬率并计算吞噬指数；ELISA法检测S2株感染后不同时间点血清中TNF-α、IL-12和IFN-γ；涂布法检测MΦ内及脾脏中的载菌量。结果：在感染后1 h，APS各剂量组腹腔MΦ吞噬率和吞噬指数均高于对照组（ P＜0.05）；在感染后1 h APS各剂量组小鼠腹腔MΦ的载菌量均高于对照组，而在感染6 h后，APS各浓度组MΦ的载菌量则明显低于对照组（ P＜0.05）；感染后6 h APS各浓度组小鼠脾内的载菌量均高于对照组，而在感染后12 h APS各浓度组脾脏载菌量又明显低于对照组；APS各浓度组血清中TNF-α、IL-12和IFN-γ的含量与对照组相比，都有显著提高（ P＜0.05）。结论：APS在体内能够促进MΦ活化，增强其吞噬和杀伤布鲁菌S2株的活性；APS可促进小鼠体内TNF-α、IL-12和IFN-γ的分泌，增强小鼠抗布鲁菌细胞免疫应答的功能。
목적：연구황기다당（ APS）대저포로균S2주감염소서면역공능적조절작용。방법：120지BALB/c소서수궤분위4조，실험조소서분별복강주사0.4、1.2、3 mg/ml적황기다당1 ml，1차/d，대조조주사동체적적생리염수，련속3 d。제4천복강주사1×107 L-1적저포로균S2주1 ml진행감염，분별우감염후1、6、12、24、48、72 h안구방혈후처사5지소서，취복강거서세포（ Macrophage ，MΦ）도편、서-희씨염색，계산감염1 h후탄서솔병계산탄서지수；ELISA법검측S2주감염후불동시간점혈청중TNF-α、IL-12화IFN-γ；도포법검측MΦ내급비장중적재균량。결과：재감염후1 h，APS각제량조복강MΦ탄서솔화탄서지수균고우대조조（ P＜0.05）；재감염후1 h APS각제량조소서복강MΦ적재균량균고우대조조，이재감염6 h후，APS각농도조MΦ적재균량칙명현저우대조조（ P＜0.05）；감염후6 h APS각농도조소서비내적재균량균고우대조조，이재감염후12 h APS각농도조비장재균량우명현저우대조조；APS각농도조혈청중TNF-α、IL-12화IFN-γ적함량여대조조상비，도유현저제고（ P＜0.05）。결론：APS재체내능구촉진MΦ활화，증강기탄서화살상포로균S2주적활성；APS가촉진소서체내TNF-α、IL-12화IFN-γ적분비，증강소서항포로균세포면역응답적공능。
To study the regulating effect of Astragalus Polysaccharides ( APS) to the mice infected by Brucella suis S2.Methods:120 BALB/c mice were randomly divided into 4 groups:experimental mice were injected APS 1 ml ( 0.4,1.2,3 mg/ml) via peritoneal cavity respectively once a day and the control group was injected with the same volume of saline for 3 days,then infected with Brucella suis S2 1 ml (1×107 L-1 ) by ip.Five mice of each group were killed through eye bloodletting at 1,6,12,24,48, 72 h respectively post-infection with Brucella suis S 2 and the peritoneal macrophage were obtained respectively to make smear.Phagocytic rate and phagocytic index were calculated by the Wright Giemsa staining after infected 1 h.TNF-α,IL-12 and IFN-γlevels of serum at different time points were measured by ELISA.The bacterial load of MΦand spleen were measured by coating method.Results:The phagocytic rate and phagocytic index of MΦin APS 3 dose groups were higher than those of the control group ( P<0.05 ).The microbial load of MΦin APS 3 dose groups at 1 h infected by Brucella suis S 2 were significantly higher than those of control,but significantly lower than those of control at 6,12,24,48,72 h after infected by Brucella suis S2.The microbial load of spleen in APS 3 dose groups at 6 h infected by Brucella suis S 2 were significantly higher than those of control ,but significantly lower than those of control at 12,24,48,72h after infected by Brucella suis S2.The concentrations of TNF-α,IL-12 and IFN-γin the serum of APS groups had significantly been improved ( P<0.05 ).Conclusion: APS can promote the activation of MΦin vivo and strengthen the activity of phagocytosis and killing to Brucella suis S 2.APS can promote the secretion of TNF-α,IL-12 and IFN-γof mice,strengthen the cellular immune response of mice to Brucella suis S 2.