中国免疫学杂志
中國免疫學雜誌
중국면역학잡지
CHINESE JOURNAL OF IMMUNOLOGY
2014年
8期
1036-1039,1045
,共5页
实验性自身免疫性脑脊髓炎%LCN2%CXCL10%多发性硬化%氟西汀
實驗性自身免疫性腦脊髓炎%LCN2%CXCL10%多髮性硬化%氟西汀
실험성자신면역성뇌척수염%LCN2%CXCL10%다발성경화%불서정
Experimental autoimmune encephalomyelitis%LCN2%CXCL10%Multiple sclerosis%Fluoxetine
目的：探讨载脂蛋白2（Lipocalin2，LCN2）、CXCL10与实验性自身免疫性脑脊髓炎（Experimental autoimmune encephalomyelitis，EAE）的关系及氟西汀在EAE中的作用。方法：通过随机数字表将实验C57BL/6小鼠随机分为对照组、EAE组、干预组及氟西汀组，每组各20只。建模前经紫外线（280～320 nm）照射，干预组及氟西汀组建立EAE动物模型。于小鼠免疫第一天起氟西汀组小鼠连续给予氟西汀（10 mg/kg）灌胃；对照组、干预组和EAE组小鼠连续给予等量生理盐水灌胃，持续至实验结束。比较各组小鼠平均发病时间、神经功能评分、发病率；采用HE染色、免疫组织化学染色法观察各组小鼠脑组织病理变化；采用酶联免疫吸附法检测血清中CXCL10含量的变化并进行比较。结果：氟西汀组小鼠平均发病时间、神经功能评分、发病率均低于干预组、EAE组（ P＜0.05）。干预组低于EAE组（ P＜0.05）。氟西汀组小鼠脑组织中炎性反应较EAE组轻；LCN2免疫组化染色显示阳性细胞秩次明显低于EAE组（ P＜0.05）。血清中CXCL10含量氟西汀组较EAE组降低（ P＜0.05）。 EAE组LCN2水平与CXCL10含量相关。结论：LCN2、CXCL10与EAE存在重要联系，氟西汀可改善EAE临床症状，缓解EAE的发病情况。
目的：探討載脂蛋白2（Lipocalin2，LCN2）、CXCL10與實驗性自身免疫性腦脊髓炎（Experimental autoimmune encephalomyelitis，EAE）的關繫及氟西汀在EAE中的作用。方法：通過隨機數字錶將實驗C57BL/6小鼠隨機分為對照組、EAE組、榦預組及氟西汀組，每組各20隻。建模前經紫外線（280～320 nm）照射，榦預組及氟西汀組建立EAE動物模型。于小鼠免疫第一天起氟西汀組小鼠連續給予氟西汀（10 mg/kg）灌胃；對照組、榦預組和EAE組小鼠連續給予等量生理鹽水灌胃，持續至實驗結束。比較各組小鼠平均髮病時間、神經功能評分、髮病率；採用HE染色、免疫組織化學染色法觀察各組小鼠腦組織病理變化；採用酶聯免疫吸附法檢測血清中CXCL10含量的變化併進行比較。結果：氟西汀組小鼠平均髮病時間、神經功能評分、髮病率均低于榦預組、EAE組（ P＜0.05）。榦預組低于EAE組（ P＜0.05）。氟西汀組小鼠腦組織中炎性反應較EAE組輕；LCN2免疫組化染色顯示暘性細胞秩次明顯低于EAE組（ P＜0.05）。血清中CXCL10含量氟西汀組較EAE組降低（ P＜0.05）。 EAE組LCN2水平與CXCL10含量相關。結論：LCN2、CXCL10與EAE存在重要聯繫，氟西汀可改善EAE臨床癥狀，緩解EAE的髮病情況。
목적：탐토재지단백2（Lipocalin2，LCN2）、CXCL10여실험성자신면역성뇌척수염（Experimental autoimmune encephalomyelitis，EAE）적관계급불서정재EAE중적작용。방법：통과수궤수자표장실험C57BL/6소서수궤분위대조조、EAE조、간예조급불서정조，매조각20지。건모전경자외선（280～320 nm）조사，간예조급불서정조건립EAE동물모형。우소서면역제일천기불서정조소서련속급여불서정（10 mg/kg）관위；대조조、간예조화EAE조소서련속급여등량생리염수관위，지속지실험결속。비교각조소서평균발병시간、신경공능평분、발병솔；채용HE염색、면역조직화학염색법관찰각조소서뇌조직병리변화；채용매련면역흡부법검측혈청중CXCL10함량적변화병진행비교。결과：불서정조소서평균발병시간、신경공능평분、발병솔균저우간예조、EAE조（ P＜0.05）。간예조저우EAE조（ P＜0.05）。불서정조소서뇌조직중염성반응교EAE조경；LCN2면역조화염색현시양성세포질차명현저우EAE조（ P＜0.05）。혈청중CXCL10함량불서정조교EAE조강저（ P＜0.05）。 EAE조LCN2수평여CXCL10함량상관。결론：LCN2、CXCL10여EAE존재중요련계，불서정가개선EAE림상증상，완해EAE적발병정황。
To investigate the correlation between LCN 2 or CXCL10 and experimental autoimmune encephalomyelitis(EAE),and the effect of Fluoxetine in EAE mice.Methods: Mice were randomly divided into four groups: control group, EAE group, intervention group and fluoxetine group , each group included twenty mice.EAE model were constructed with MOG35-55 in the intervention group and fluoxetine group ,following ultraviolet (UV) (280-320 nm) irradiation.Mice in fluoxetine group were given fluoxetine (10 mg/kg) by daily gavage since immunized ,and the saline was used in the control group ,intervention group and EAE group in the same way and same time.The drug/saline was continuously administered from the immunization to the day mice were sacrificed.The mean attacked time ,the nerve function grades and the incidence were observed and compared.We observed brain pathological changes by HE staining and immunohistochemistry;CXCL10 were tested by ELISA method ,and compared among the four groups.Results:The mean attacked time ,the nerve function grades and the incidence in the fluoxetine group were lower than those in the intervention group and EAE group ,there were significant differences between two groups (P<0.05),in the intervention group were lower than those in the EAE group ,there were significant differences between two groups ( P<0.05 ).Fluoxetine treated EAE mice showed decreased degree of inflammatory infiltration;the mean rank of positive cells lower in the fluoxetine group than those in the EAE group,the differences were significant between two groups ( P<0.05 ).The expression of CXCL10 in the peripheral blood plasma of fluoxetine group were lower than those in the EAE group ,there were significant differences between two groups ( P<0.05 ).Levels of LCN2 expression were correlated with those of CXCL 10 in the EAE group.Conclusion: LCN2, CXCL10 are correlated with the pathogenesis of EAE.Fluoxetine could alleviate clinical symptoms of EAE and alleviate the morbidity of EAE.
