CAJ | 학술논문

目的：观察livin基因siRNA对鼻咽癌细胞CNE-2Z增殖和凋亡的影响。方法：将靶向livin基因的siRNA真核表达质粒pGPU6/GFP/Neo-livin通过脂质体介导转染至鼻咽癌细胞系CNE-2Z，半定量RT-PCR、Western blot分别检测livin基因mRNA和蛋白表达，caspase-3活性检测试剂盒检测caspase-3的活性，MTT法检测细胞增殖活性，流式细胞仪检测细胞凋亡率。结果：与未转染（空白对照）和阴性对照组（ pGPU6/GPF/Neo-shNC，无关序列对照组） CNE-2Z细胞比较，实验组（ pGPU6/GPF/Neo-livin）重组质粒使livin基因mRNA和蛋白表达明显下降，表达抑制率分别为64.38％和61.43％；caspase-3活性增强，细胞增殖活力受到抑制，细胞凋亡率增加。结论：靶向livin的siRNA抑制CNE-2Z细胞增殖并促进其凋亡。
목적：관찰livin기인siRNA대비인암세포CNE-2Z증식화조망적영향。방법：장파향livin기인적siRNA진핵표체질립pGPU6/GFP/Neo-livin통과지질체개도전염지비인암세포계CNE-2Z，반정량RT-PCR、Western blot분별검측livin기인mRNA화단백표체，caspase-3활성검측시제합검측caspase-3적활성，MTT법검측세포증식활성，류식세포의검측세포조망솔。결과：여미전염（공백대조）화음성대조조（ pGPU6/GPF/Neo-shNC，무관서렬대조조） CNE-2Z세포비교，실험조（ pGPU6/GPF/Neo-livin）중조질립사livin기인mRNA화단백표체명현하강，표체억제솔분별위64.38％화61.43％；caspase-3활성증강，세포증식활력수도억제，세포조망솔증가。결론：파향livin적siRNA억제CNE-2Z세포증식병촉진기조망。
To observe the effect of livin gene-specific siRNA interference on proliferation and apoptosis of nasopharyngeal carcinoma (NPC) cell line CNE-2Z.Methods: siRNA expression vectors pGPU6/GFP/Neo-livin were transfected into NPC cell line CNE-2Z by using Lipofectamine 2000.The expressions of livin mRNA and protein were detected by semi-quantitative reverse transcriptase-polymerase chain reaction ( RT-PCR) and Western blot.The changes of caspase-3 activity were assessed by kinase semi-quantitative activity test.The proliferation activity and apoptosis of CNE-2Z cells were examined by MTT and flow cytometry respectively.Results:The expression levels of livin mRNA and protein in pGPU 6/GPF/Neo-livin transfected CNE-2Z cells were significantly lower than those in untreated and pGPU 6/GFP/Neo-shNC ( control non-target siRNA ) transfected cells with the expression inhibitory rate of 64.38% and 61.43% respectively.The caspase-3 activity and the apoptotic rate of experimental group cells were increased obviously.The growth of CNE-2Z cells was inhibited by siRNA recombinant expression vector transfection.Conclusion:siRNA targeting livin gene inhibits proliferation and induce apoptosis of CNE-2Z cells.