CAJ | 학술논문

目的：探讨蛋白异天冬氨酰甲基转移酶（Protein isoaspartyl-methyltransferase，PIMT）过表达对类风湿关节炎（Rheumatoid arthritis，RA）成纤维样滑膜细胞（Fibroblast-like synoviocytes，FLS）凋亡的影响。方法：构建PIMT表达载体，转染RA-FLS细胞，检测PIMT在RA-FLS细胞表达水平，观察转染后RA-FLS细胞的凋亡情况。结果：转染后的RA-FLS细胞PIMT mRNA和蛋白质表达水平上升，与正常对照和转染空载体的RA-FLS细胞阴性对照相比较，转然后RA-FLS细胞的凋亡增加。结论：PIMT在RA-FLS细胞中表达下降是RA-FLS细胞凋亡减少的一个重要原因，PIMT在RA-FLS细胞增殖/凋亡失调中起作用。
목적：탐토단백이천동안선갑기전이매（Protein isoaspartyl-methyltransferase，PIMT）과표체대류풍습관절염（Rheumatoid arthritis，RA）성섬유양활막세포（Fibroblast-like synoviocytes，FLS）조망적영향。방법：구건PIMT표체재체，전염RA-FLS세포，검측PIMT재RA-FLS세포표체수평，관찰전염후RA-FLS세포적조망정황。결과：전염후적RA-FLS세포PIMT mRNA화단백질표체수평상승，여정상대조화전염공재체적RA-FLS세포음성대조상비교，전연후RA-FLS세포적조망증가。결론：PIMT재RA-FLS세포중표체하강시RA-FLS세포조망감소적일개중요원인，PIMT재RA-FLS세포증식/조망실조중기작용。
To prove the influence of protein isoaspartyl-methyltransferase ( PIMT ) on the cell apoptosis of fibroblast-like synoviocytes of RA.Methods: The expression vector of PIMT was constructed and transfected in to the RA-FLS, the impact of PIMT on the cell apoptosis of RA-FLS was observed by overexpressing the vector of PIMT.Results:The mRNA and protein level of PIMT in RA-FLS was increased after transfected the vector of PIMT into RA-FLS;compared with the normal cultured RA-FLS and the RA-FLS transfected with the empty vectors ,the cell apoptosis level was also increased.Conclusion:The decreased expression level of PIMT in RA-FLS is an important reason for reduce apoptosis of RA-FLS,and PIMT can affect the imbalance of proliferation/ap-optosis in the RA-FLS.