中国康复理论与实践
中國康複理論與實踐
중국강복이론여실천
CHINESE JOURNAL OF REHABILITATION THEORY & PRACTICE
2014年
8期
718-722
,共5页
龙志华%高飞%张锋良%岳军忠%王磊%王烨%刘文国%徐青
龍誌華%高飛%張鋒良%嶽軍忠%王磊%王燁%劉文國%徐青
룡지화%고비%장봉량%악군충%왕뢰%왕엽%류문국%서청
脊髓损伤%P物质%神经源性肠道功能障碍%大鼠
脊髓損傷%P物質%神經源性腸道功能障礙%大鼠
척수손상%P물질%신경원성장도공능장애%대서
spinal cord injury%substance P%neurogenic bowel dysfunction%rats
目的:探讨脊髓损伤后结肠中P物质与神经源性肠道功能障碍的关系。方法60只体质量(220±40) g的雄性Sprague-Dawley大鼠随机分为假手术组(n=20)、正常对照组(n=20)和模型组(n=20)。氯胺酮60 mg/kg腹腔注射麻醉大鼠,利用NYU脊髓打击器,以75 g?cm致伤力制作T10脊髓损伤模型,分别于造模后24 h、1周、3周和5周时切除大鼠结肠组织制作标本,检测肠道传输功能,采用ELISA方法测定血清中和组织中的P物质含量,实时荧光定量PCR和Western blotting法检测P物质mRNA和蛋白表达。结果模型组大鼠脊髓损伤后出现肠道传输功能下降,且于造模后3周时肠道传输达到最低值;造模后3周时模型组血清和组织中P物质含量与假手术组相比均降低,结肠组织中P物质的mRNA及蛋白表达水平也下调,与假手术组、正常对照组相比具有显著性差异,假手术组P物质的表达是模型组的(3.12±0.51)倍(P<0.05)。结论大鼠脊髓损伤后神经源性肠道功能障碍与结肠中P物质的表达降低有关。
目的:探討脊髓損傷後結腸中P物質與神經源性腸道功能障礙的關繫。方法60隻體質量(220±40) g的雄性Sprague-Dawley大鼠隨機分為假手術組(n=20)、正常對照組(n=20)和模型組(n=20)。氯胺酮60 mg/kg腹腔註射痳醉大鼠,利用NYU脊髓打擊器,以75 g?cm緻傷力製作T10脊髓損傷模型,分彆于造模後24 h、1週、3週和5週時切除大鼠結腸組織製作標本,檢測腸道傳輸功能,採用ELISA方法測定血清中和組織中的P物質含量,實時熒光定量PCR和Western blotting法檢測P物質mRNA和蛋白錶達。結果模型組大鼠脊髓損傷後齣現腸道傳輸功能下降,且于造模後3週時腸道傳輸達到最低值;造模後3週時模型組血清和組織中P物質含量與假手術組相比均降低,結腸組織中P物質的mRNA及蛋白錶達水平也下調,與假手術組、正常對照組相比具有顯著性差異,假手術組P物質的錶達是模型組的(3.12±0.51)倍(P<0.05)。結論大鼠脊髓損傷後神經源性腸道功能障礙與結腸中P物質的錶達降低有關。
목적:탐토척수손상후결장중P물질여신경원성장도공능장애적관계。방법60지체질량(220±40) g적웅성Sprague-Dawley대서수궤분위가수술조(n=20)、정상대조조(n=20)화모형조(n=20)。록알동60 mg/kg복강주사마취대서,이용NYU척수타격기,이75 g?cm치상력제작T10척수손상모형,분별우조모후24 h、1주、3주화5주시절제대서결장조직제작표본,검측장도전수공능,채용ELISA방법측정혈청중화조직중적P물질함량,실시형광정량PCR화Western blotting법검측P물질mRNA화단백표체。결과모형조대서척수손상후출현장도전수공능하강,차우조모후3주시장도전수체도최저치;조모후3주시모형조혈청화조직중P물질함량여가수술조상비균강저,결장조직중P물질적mRNA급단백표체수평야하조,여가수술조、정상대조조상비구유현저성차이,가수술조P물질적표체시모형조적(3.12±0.51)배(P<0.05)。결론대서척수손상후신경원성장도공능장애여결장중P물질적표체강저유관。
Objective To investigate the relationship between neurogenic bowel dysfunction (NBD) and substance P in rats suffering from spinal cord injury (SCI). Methods 60 male Sprague-Dawley rats, weighted (220±40) g, were randomly divided into three groups:sham group (n=20), normal group (n=20) and model group (n=20) and then were subdivided into subgroups of 24 h, 1 week, 3 weeks, and 5 weeks respectively after SCI. SCI model was established at thoracic 10 segment of rat with NYU impactor device. The colon tissue of the rats was resected and stored. Substance P in serum and tissue was measured by ELISA. The tissue was examined by real-time RT-PCR and Western blotting to analyze the expression of substance P. Results The colon intestinal transmission function decreased and delineated at minimum value at 3 weeks in the model group. There was statistical significance with respect to the content of substance P in serum and tis-sue between the sham group and model group at 3 weeks. The expression of substance P in the sham group was (3.12 ± 0.51) times of the model group (P<0.05). Conclusion Substance P may take part in NBD after SCI in rats.