CAJ | 학술논문

目的：观察原花青素对脊髓损伤后大鼠核因子-кB (NF-кB)、白细胞介素-6(IL-6)表达变化的影响。方法36只健康成年Sprague-Dawley大鼠均分为3组：原花青素治疗组(A组)、甲基泼尼松龙治疗组(B组)和对照组(C组)。采用Allen法复制大鼠T9急性脊髓损伤模型，于术后1 d、3 d和7 d对各组大鼠行后肢运动功能BBB评分和斜板试验；免疫组织化学染色检测脊髓NF-кB和IL-6的表达。结果术后3 d、7 d，A组、B组大鼠BBB评分和斜板试验成绩均优于C组(P<0.05)。A组术后各时间点NF-кB表达均明显低于C组(P<0.01)，B组术后3 d、7 d的NF-кB表达强度明显低于C组(P<0.01)。术后各时间点，A、B两组IL-6表达均低于C组(P<0.05)。结论原花青素可抑制脊髓损伤后大鼠脊髓组织NF-кB、IL-6的表达，有效抑制炎症反应，从而促进大鼠后肢运动功能的恢复。
목적：관찰원화청소대척수손상후대서핵인자-кB (NF-кB)、백세포개소-6(IL-6)표체변화적영향。방법36지건강성년Sprague-Dawley대서균분위3조：원화청소치료조(A조)、갑기발니송룡치료조(B조)화대조조(C조)。채용Allen법복제대서T9급성척수손상모형，우술후1 d、3 d화7 d대각조대서행후지운동공능BBB평분화사판시험；면역조직화학염색검측척수NF-кB화IL-6적표체。결과술후3 d、7 d，A조、B조대서BBB평분화사판시험성적균우우C조(P<0.05)。A조술후각시간점NF-кB표체균명현저우C조(P<0.01)，B조술후3 d、7 d적NF-кB표체강도명현저우C조(P<0.01)。술후각시간점，A、B량조IL-6표체균저우C조(P<0.05)。결론원화청소가억제척수손상후대서척수조직NF-кB、IL-6적표체，유효억제염증반응，종이촉진대서후지운동공능적회복。
Objective To observe the effect of proanthocyanidin on the expression of nuclear factor kappa B (NF-кB) and interleukin-6 (IL-6) in rats with spinal cord injury (SCI). Methods 36 healthy adult Sprague-Dawley rats were randomly divided into proanthocyanidin treatment group (group A, n=12), methylprednisolone (MP) treatment group (group B, n=12) and model control group (group C, n=12). Al-len's method was used to establish the model of acute spinal cord injury in T9. On the 1st, 3rd and 7th days after operation, 4 rats in each group were assessed with Basso-Beattie-Bresnahan (BBB) scale and Slanting Board Test (SBT), and the expression of NF-кB and IL-6 of the spinal cord were detected with immunohistochemistry. Results The scores of BBB and SBT were better in groups A and B than in the group C 3 and 7 days after SCI (P<0.05). Compared with group C, the expression of NF-кB in group A decreased all the time after SCI (P<0.01), and 3 and 7 days after SCI in group B (P<0.01);while the expression of IL-6 decreased all the time after SCI in groups A and B (P<0.05). Conclusion Proanthocyanidin may inhibit the expression of NF-кB and IL-6 in spinal cord after SCI in order to reduce inflammation and improve the hindlimb motor function in rats after SCI.