中国急救医学
中國急救醫學
중국급구의학
CHINESE JOURNAL OF CRITICAL CARE MEDICINE
2014年
8期
729-733
,共5页
叶媛%何婧%戚迪%冯龙华%王导新
葉媛%何婧%慼迪%馮龍華%王導新
협원%하청%척적%풍룡화%왕도신
过氧化物酶增殖体活化受体-γ(PPAR-γ)%罗格列酮%急性呼吸窘迫综合征(ARDS)%上皮细胞钠通道(ENaC)
過氧化物酶增殖體活化受體-γ(PPAR-γ)%囉格列酮%急性呼吸窘迫綜閤徵(ARDS)%上皮細胞鈉通道(ENaC)
과양화물매증식체활화수체-γ(PPAR-γ)%라격렬동%급성호흡군박종합정(ARDS)%상피세포납통도(ENaC)
Peroxisome proliferator activated receptor -γ(PPAR-γ)%Rosiglitazone%Acute respiratory distress syndrome (ARDS)%Epithelial sodium channel (ENaC)
目的：探讨过氧化物酶增殖体活化受体-γ（ PPAR-γ）激动剂对LPS诱导的小鼠ARDS的保护作用及其对肺泡上皮细胞钠通道-γ亚基（ ENaC-γ）的调控作用。方法40只雄性BALB/C小鼠随机分为对照组（ Control组）、模型组（ LPS组）、罗格列酮治疗组（ RGZ组，LPS＋罗格列酮）及GW9662干预组（ GW9662组，LPS＋GW9662＋罗格列酮），每组10只。处理后收集相关标本，ELISA测定肺泡灌洗液（ BALF）中IL-1β和TNF-α水平。肺组织湿/干比（ W/D）测定肺水肿程度。 HE染色观察肺组织病理变化并进行肺组织损伤评分。用免疫组织化学法和Western blot测定肺组织ENaC-γ蛋白的表达。 RT-PCR测定ENaC-γmRNA的表达。结果与Control组比较，LPS组、RGZ组和GW9662组中IL-1β和TNF-α水平、W/D值、HE染色肺组织损伤程度及评分明显升高（ P＜0．05）；与LPS组比较，RGZ组各指标水平及肺损伤评分明显下降（P＜0．05），但LPS组和GW9662组比较差异无统计学意义（P＞0．05）。 LPS造模后，LPS组（210±15IOD，0．18±0．02，0．22±0．04）、RGZ组（450±30IOD，0．43±0．03，0．45±0．03）、GW9662组（235±35IOD，0．21±0．03，0．25±0．02）肺组织ENaC-γ蛋白和mRNA的表达明显低于Control组（600±25IOD，0．64±0．01，0．67±0．02，P＜0．05），RGZ组与LPS组和GW9662比较差异有统计学意义（P＜0．05），但LPS组和GW9662组比较差异无统计学意义（P＞0．05）。结论 PPAR-γ受体激动剂可能从转录水平上调肺泡ENaC-γ的表达，从而减轻LPS诱导的小鼠ARDS肺水肿程度，发挥保护性作用。
目的：探討過氧化物酶增殖體活化受體-γ（ PPAR-γ）激動劑對LPS誘導的小鼠ARDS的保護作用及其對肺泡上皮細胞鈉通道-γ亞基（ ENaC-γ）的調控作用。方法40隻雄性BALB/C小鼠隨機分為對照組（ Control組）、模型組（ LPS組）、囉格列酮治療組（ RGZ組，LPS＋囉格列酮）及GW9662榦預組（ GW9662組，LPS＋GW9662＋囉格列酮），每組10隻。處理後收集相關標本，ELISA測定肺泡灌洗液（ BALF）中IL-1β和TNF-α水平。肺組織濕/榦比（ W/D）測定肺水腫程度。 HE染色觀察肺組織病理變化併進行肺組織損傷評分。用免疫組織化學法和Western blot測定肺組織ENaC-γ蛋白的錶達。 RT-PCR測定ENaC-γmRNA的錶達。結果與Control組比較，LPS組、RGZ組和GW9662組中IL-1β和TNF-α水平、W/D值、HE染色肺組織損傷程度及評分明顯升高（ P＜0．05）；與LPS組比較，RGZ組各指標水平及肺損傷評分明顯下降（P＜0．05），但LPS組和GW9662組比較差異無統計學意義（P＞0．05）。 LPS造模後，LPS組（210±15IOD，0．18±0．02，0．22±0．04）、RGZ組（450±30IOD，0．43±0．03，0．45±0．03）、GW9662組（235±35IOD，0．21±0．03，0．25±0．02）肺組織ENaC-γ蛋白和mRNA的錶達明顯低于Control組（600±25IOD，0．64±0．01，0．67±0．02，P＜0．05），RGZ組與LPS組和GW9662比較差異有統計學意義（P＜0．05），但LPS組和GW9662組比較差異無統計學意義（P＞0．05）。結論 PPAR-γ受體激動劑可能從轉錄水平上調肺泡ENaC-γ的錶達，從而減輕LPS誘導的小鼠ARDS肺水腫程度，髮揮保護性作用。
목적：탐토과양화물매증식체활화수체-γ（ PPAR-γ）격동제대LPS유도적소서ARDS적보호작용급기대폐포상피세포납통도-γ아기（ ENaC-γ）적조공작용。방법40지웅성BALB/C소서수궤분위대조조（ Control조）、모형조（ LPS조）、라격렬동치료조（ RGZ조，LPS＋라격렬동）급GW9662간예조（ GW9662조，LPS＋GW9662＋라격렬동），매조10지。처리후수집상관표본，ELISA측정폐포관세액（ BALF）중IL-1β화TNF-α수평。폐조직습/간비（ W/D）측정폐수종정도。 HE염색관찰폐조직병리변화병진행폐조직손상평분。용면역조직화학법화Western blot측정폐조직ENaC-γ단백적표체。 RT-PCR측정ENaC-γmRNA적표체。결과여Control조비교，LPS조、RGZ조화GW9662조중IL-1β화TNF-α수평、W/D치、HE염색폐조직손상정도급평분명현승고（ P＜0．05）；여LPS조비교，RGZ조각지표수평급폐손상평분명현하강（P＜0．05），단LPS조화GW9662조비교차이무통계학의의（P＞0．05）。 LPS조모후，LPS조（210±15IOD，0．18±0．02，0．22±0．04）、RGZ조（450±30IOD，0．43±0．03，0．45±0．03）、GW9662조（235±35IOD，0．21±0．03，0．25±0．02）폐조직ENaC-γ단백화mRNA적표체명현저우Control조（600±25IOD，0．64±0．01，0．67±0．02，P＜0．05），RGZ조여LPS조화GW9662비교차이유통계학의의（P＜0．05），단LPS조화GW9662조비교차이무통계학의의（P＞0．05）。결론 PPAR-γ수체격동제가능종전록수평상조폐포ENaC-γ적표체，종이감경LPS유도적소서ARDS폐수종정도，발휘보호성작용。
Objective To investigate the effect of PPAR -γagonist on the expression of epithelial sodium channel -γ( ENaC-γ) in mice with LPS-induced ARDS.Methods Forty male BALB/C mice were randomly divided into Control group , LPS group, RGZ group and GW9662 group ( n=10 ) .The levels of interleukin -1β( IL-1β) and tumor necrosis factor -α( TNF-α) in the bronchoalveolar lavage fluid ( BALF) were measured by ELISA .The wet-to-dry ratio ( W/D) of the lung tissues was determined to evaluate lung edema .HE staining was used to observe the pathological changes and to assess lung injury scores .The expressions of ENaC -γprotein in lung tissues were detected by immunohistochemistry and Western blotting .The levels of ENaC -γmRNA in the lung tissues were analyzed using RT -PCR.Results Compared with those in Control group , the IL-1βand TNF-αlevels in the BALF, W/D, lung injury scores were significantly increased in LPS group , RGZ group and GW9662 group (P<0.05).Compared with LPS group, the index levels and lung injury scores were significantly decreased in RGZ group ( P <0.05 ).However, there was no statistical difference between LPS group and GW9662 group (P>0.05).Compared with Control group (600 ± 25IOD, 0.64 ±0.01, 0.67 ±0.02), the expressions of ENaC -γprotein and mRNA were significantly decreased in LPS group (210 ±15IOD, 0.18 ±0.02, 0.22 ±0.04; P<0.05), RGZ group (450 ± 30IOD, 0.43 ±0.03, 0.45 ±0.03;P<0.05) and GW9662 group (235 ±35IOD, 0.21 ±0.03, 0.25 ± 0.02;P <0.05).The expression of ENaC -γprotein and mRNA in RGZ group were significantly different from those in LPS group and GW9662 group ( P <0.05 ), but there were no statistical differences between LPS group and GW9662 group (P>0.05).Conclusion PPAR-γagonist may play a protective role in mice with LPS -induced ARDS via up -regulation of ENaC -γat the transcriptional level .
