CAJ | 학술논문

目的通过检测支气管肺泡灌洗液(BALF)中白细胞介素-25(IL-25)和γ-干扰素(IFN-γ)的水平,探讨地塞米松对小鼠支气管哮喘的治疗作用机制。方法清洁级Balb/c小鼠随机分为正常对照组、哮喘组和地塞米松组。以鸡卵清蛋白( OVA)致敏激发法建立哮喘小鼠模型。地塞米松组在每次激发前1 h 予地塞米松腹腔注射。每次激发时观察小鼠的表现。于末次激发24 h处死小鼠,取右肺作苏木精-×红( HE)染色病理切片,显微镜下观察炎症情况；收集左肺BALF,镜下计数白细胞总数、嗜酸性粒细胞( EOS)绝对数目,计算EOS百分比；用酶联免疫吸附法( ELISA)测定BALF中IL-25和IFN-γ的水平,并做相关性分析。结果哮喘组小鼠BALF中白细胞总数、EOS数目和百分比分别与正常对照组、地塞米松组比较均明显增加(P<0.05),而以上3个指标在正常对照组和地塞米松组间差异无统计学意义。哮喘组小鼠BALF中IL-25水平高于正常对照组和地塞米松组(P<0.05),而地塞米松组的含量也高于正常对照组；IFN-γ水平低于正常对照组和地塞米松组(P<0.05),而后两组差异无统计学意义。各组小鼠BALF中IL-25和IFN-γ水平都呈负相关。结论地塞米松治疗哮喘病的部分机制是减轻肺部炎症和促进IFN-γ的产生,同时可能抑制IL-25的表达。
목적통과검측지기관폐포관세액(BALF)중백세포개소-25(IL-25)화γ-간우소(IFN-γ)적수평,탐토지새미송대소서지기관효천적치료작용궤제。방법청길급Balb/c소서수궤분위정상대조조、효천조화지새미송조。이계란청단백( OVA)치민격발법건립효천소서모형。지새미송조재매차격발전1 h 여지새미송복강주사。매차격발시관찰소서적표현。우말차격발24 h처사소서,취우폐작소목정-×홍( HE)염색병리절편,현미경하관찰염증정황；수집좌폐BALF,경하계수백세포총수、기산성립세포( EOS)절대수목,계산EOS백분비；용매련면역흡부법( ELISA)측정BALF중IL-25화IFN-γ적수평,병주상관성분석。결과효천조소서BALF중백세포총수、EOS수목화백분비분별여정상대조조、지새미송조비교균명현증가(P<0.05),이이상3개지표재정상대조조화지새미송조간차이무통계학의의。효천조소서BALF중IL-25수평고우정상대조조화지새미송조(P<0.05),이지새미송조적함량야고우정상대조조；IFN-γ수평저우정상대조조화지새미송조(P<0.05),이후량조차이무통계학의의。각조소서BALF중IL-25화IFN-γ수평도정부상관。결론지새미송치료효천병적부분궤제시감경폐부염증화촉진IFN-γ적산생,동시가능억제IL-25적표체。
Objective To investigate the mechanism of therapeutic action of dexamethasone on asthmatic mice by detecting the levels of IL-25 and IFN-γ in bronchoalveolar lavage fluid (BALF). Methods Balb/c mice with SPF grade were randomly divided into normal control group, asthma group and dexamethasone group. Asthma group and dexamethasone group were sensitized and challenged with ovalbumin ( OVA) . Dexamethasone group was intraperitoneally injected with dexamethasone one hour before challenging. The mice were executed 24 hours after the last challenge, and the HE stained pathological sections of the right lung were made. Pathological sections of lung were observed. BALF in the left lung was also collected. The total white blood cell count and absolute eosinophile ( EOS) count were observed, and the percentage of EOS was calculated. The levels of IL-25 and IFN-γwere measured with ELISA, and correlation analyses were made. Results The counts of total white blood cell and EOS, and the percentage of EOS were significantly higher in the asthma group than in the normal control group and dexamethasone group (P<0. 05). No differences were found between the normal control group and dexamethasone group. The IL-25 level was higher in the asthma group than in the normal control group and dexamethasone group (P<0. 05), and its level in the dexamethasone group was also higher than that in the normal control group. The IFN-γlevel was lower in the asthma group than in the normal control group and dexamethasone group (P<0. 05), while there was no significant difference between the normal control group and dexamethasone group. IL-25 was negatively correlated with IFN-γin each group. Conclusion Part of the mechanisms of dexamethasone acting on asthma are related to its inhibition on the pulmonary inflammation and promotion on the expression of IFN-γ, and possible inhibition of IL-25 expression.