CAJ | 학술논문

目的：比较免疫组化（IHC）和荧光原位杂交（FISH）技术在检测乳腺浸润性癌患者中 HER -2蛋白表达和基因扩增的一致性。方法：分别用 IHC 和 FISH 技术对172例乳腺浸润性癌 HER -2进行蛋白表达和基因扩增检测，比较两者检测的结果和相关性。结果：172例浸润性乳腺癌标本行 IHC 检测结果30例为（1+），88例为（2+），42例为（3+），12例为（-）。172例乳腺癌标本进行 FISH 检测结果71例为阳性，101例为阴性。其中 FISH 结果阳性标本中 IHC 检测有2例（1+），30例（2+），39例（3+）。IHC 检测 HER -2为（3+）的病例 FISH 检测中阳性符合率92.9%（39/42），且检测 HER -2（-）的病例 FISH 检测均为阴性， IHC 检测 HER -2（2+）的88例患者中有58例经 FISH 检测证实 HER -2呈阴性，30例呈阳性。FISH 检测共发现47例17号染色体多体，其发生率为27.3%。用 IHC 检测 HER -2发现有25例标本存在肿瘤之间的不同表达，而在这25例标本的 FISH 检测结果中有11例存在 HER -2基因瘤内遗传异质性。结论：HER -2蛋白表达和基因扩增 IHC 和 FISH 检测在免疫组化强阳性的标本中具有较好的一致性，且免疫组化染色强度与HER -2基因扩增呈正相关。理解和判断 HER -2基因遗传异质性对肿瘤药物应用及 HER -2基因检测方法具有指导意义。
목적：비교면역조화（IHC）화형광원위잡교（FISH）기술재검측유선침윤성암환자중 HER -2단백표체화기인확증적일치성。방법：분별용 IHC 화 FISH 기술대172례유선침윤성암 HER -2진행단백표체화기인확증검측，비교량자검측적결과화상관성。결과：172례침윤성유선암표본행 IHC 검측결과30례위（1+），88례위（2+），42례위（3+），12례위（-）。172례유선암표본진행 FISH 검측결과71례위양성，101례위음성。기중 FISH 결과양성표본중 IHC 검측유2례（1+），30례（2+），39례（3+）。IHC 검측 HER -2위（3+）적병례 FISH 검측중양성부합솔92.9%（39/42），차검측 HER -2（-）적병례 FISH 검측균위음성， IHC 검측 HER -2（2+）적88례환자중유58례경 FISH 검측증실 HER -2정음성，30례정양성。FISH 검측공발현47례17호염색체다체，기발생솔위27.3%。용 IHC 검측 HER -2발현유25례표본존재종류지간적불동표체，이재저25례표본적 FISH 검측결과중유11례존재 HER -2기인류내유전이질성。결론：HER -2단백표체화기인확증 IHC 화 FISH 검측재면역조화강양성적표본중구유교호적일치성，차면역조화염색강도여HER -2기인확증정정상관。리해화판단 HER -2기인유전이질성대종류약물응용급 HER -2기인검측방법구유지도의의。
To evaluate the consistency of HER - 2 detection with immunohistochemistry(IHC)and fluorescence in situ hybridization(FISH)in invasion breast carcinoma. Methods:All 172 cases of invasive breast carcinoma were detected by IHC and FISH at the same time. Results:There were 30 cases with(1 + ),88 cases with (2 + ),42 cases with(3 + )and 12 cases with( - )by IHC in the 172 invasive breast carcinoma cases,whereas 71 cases were positive and 101 cases were negative by FISH detection. In the FISH positive cases there were 2 cases with (1 + ),30 cases with(2 + )and 39 cases with(3 + )by IHC. Positive coincidence rate of cases with(3 + )by IHC was 92. 9%(39 / 42)accrording to FISH detection,cases with( - )by IHC were also detected negative by FISH de-tection,while in the 88 cases of(2 + ),there were 58 cases confirmed negative and the rest were positive by FISH de-tection. The chromosome 17 multi - body was found in 47 cases with the 27. 3% incidence rate. Different HER - 2 ex-pression was found in 25 cases of tumor specimens,in which there were 11 cases presence of genetic heterogeneity by FISH detection. Conclusion:The detections of IHC and FISH are with high coincidence rate and consistency,IHC is positively correlated with FISH. It is significant to understand and master HER - 2 genetic heterogeneity while using drug and HER - 2 gene detection methods.