现代肿瘤医学
現代腫瘤醫學
현대종류의학
JOURNAL OF MODERN ONCOLOGY
2014年
8期
1782-1785
,共4页
于秀月%孔垂泽%张哲%李振华
于秀月%孔垂澤%張哲%李振華
우수월%공수택%장철%리진화
CDC25B%肾细胞癌%ACHN%769 - P
CDC25B%腎細胞癌%ACHN%769 - P
CDC25B%신세포암%ACHN%769 - P
CDC25B%renal cell carcinoma%ACHN%769 - P
目的：探讨 CDC25B 在肾癌组织和细胞中的表达状况及对肾癌细胞凋亡、运动以及侵袭力的影响。方法：免疫组化检测肾癌组织中 CDC25B 的表达，Western blot 和 RT - PCR 检测 CDC25B 蛋白在肾癌769- P和 ACHN 细胞中的表达。挑选表达量大的细胞系转染 CDC25B shRNA。MTT 法检测细胞增殖能力。Annexin V - FITC/ PI - FCM 实验检测肾癌细胞凋亡。Transwell 检测肾癌细胞侵袭力。结果：CDC25B 在肾癌组织中高表达。CDC25B 在肾癌769- P 和 ACHN 细胞中均表达，ACHN 的恶性度高于769- P，CDC25B 在 ACHN 中表达高于769- P。CDC25B 蛋白被干扰后，随着时间的延长，肾癌 ACHN 细胞的增殖能力显著降低，各时间组与浓度组之间差异具有统计学意义。CDC25B 蛋白被干扰后，肾癌 ACHN 细胞的凋亡增加。体外运动减弱。小室侵袭实验表明，干扰组的细胞数低于对照组，差异具有统计学意义（ P ﹤0.05）。干扰后降低了肿瘤细胞的侵袭能力。结论：CDC25B 在肾癌中的表现为原癌基因的作用，CDC25B 与肾癌的恶性程度呈正相关。CDC25B 可能成为肾癌潜在的诊断标记和新的判定肿瘤恶性度的指标。
目的：探討 CDC25B 在腎癌組織和細胞中的錶達狀況及對腎癌細胞凋亡、運動以及侵襲力的影響。方法：免疫組化檢測腎癌組織中 CDC25B 的錶達，Western blot 和 RT - PCR 檢測 CDC25B 蛋白在腎癌769- P和 ACHN 細胞中的錶達。挑選錶達量大的細胞繫轉染 CDC25B shRNA。MTT 法檢測細胞增殖能力。Annexin V - FITC/ PI - FCM 實驗檢測腎癌細胞凋亡。Transwell 檢測腎癌細胞侵襲力。結果：CDC25B 在腎癌組織中高錶達。CDC25B 在腎癌769- P 和 ACHN 細胞中均錶達，ACHN 的噁性度高于769- P，CDC25B 在 ACHN 中錶達高于769- P。CDC25B 蛋白被榦擾後，隨著時間的延長，腎癌 ACHN 細胞的增殖能力顯著降低，各時間組與濃度組之間差異具有統計學意義。CDC25B 蛋白被榦擾後，腎癌 ACHN 細胞的凋亡增加。體外運動減弱。小室侵襲實驗錶明，榦擾組的細胞數低于對照組，差異具有統計學意義（ P ﹤0.05）。榦擾後降低瞭腫瘤細胞的侵襲能力。結論：CDC25B 在腎癌中的錶現為原癌基因的作用，CDC25B 與腎癌的噁性程度呈正相關。CDC25B 可能成為腎癌潛在的診斷標記和新的判定腫瘤噁性度的指標。
목적：탐토 CDC25B 재신암조직화세포중적표체상황급대신암세포조망、운동이급침습력적영향。방법：면역조화검측신암조직중 CDC25B 적표체，Western blot 화 RT - PCR 검측 CDC25B 단백재신암769- P화 ACHN 세포중적표체。도선표체량대적세포계전염 CDC25B shRNA。MTT 법검측세포증식능력。Annexin V - FITC/ PI - FCM 실험검측신암세포조망。Transwell 검측신암세포침습력。결과：CDC25B 재신암조직중고표체。CDC25B 재신암769- P 화 ACHN 세포중균표체，ACHN 적악성도고우769- P，CDC25B 재 ACHN 중표체고우769- P。CDC25B 단백피간우후，수착시간적연장，신암 ACHN 세포적증식능력현저강저，각시간조여농도조지간차이구유통계학의의。CDC25B 단백피간우후，신암 ACHN 세포적조망증가。체외운동감약。소실침습실험표명，간우조적세포수저우대조조，차이구유통계학의의（ P ﹤0.05）。간우후강저료종류세포적침습능력。결론：CDC25B 재신암중적표현위원암기인적작용，CDC25B 여신암적악성정도정정상관。CDC25B 가능성위신암잠재적진단표기화신적판정종류악성도적지표。
To investigate the expression of CDC25B in renal cell carcinoma tissues and renal carcino-ma cells. To reveal the important role of CDC25B in renal carcinoma cell proliferation,apoptosis and invasiveness. Methods:Western blot and RT - PCR were used to detect the expression of CDC25B protein in renal cell carcinoma 769 - P and ACHN cells. We select the cell lines with the higher expression transfected with CDC25B shRNA. MTT was used to assay cell proliferation. Annexin V - FITC/ PI - FCM experiment was used to detect the apoptosis of renal cell carcinoma. Tanswell was used to detect renal cell carcinoma invasiveness. Results:CDC25B protein showed a high level of expression in tumor. CDC25B expressed both in renal cell carcinoma 769 - P and ACHN cells. The de-gree of malignancy of ACHN was higher than 769 - P. The expression of CDC25B in ACHN was higher than that in 769 - P. After the interference of CDC25B protein,with time lost,the cell proliferation of renal cell carcinoma ACHN was significantly decreased,a statistically significant difference was found between each time group and the concentra-tion group. The apoptosis of renal cell carcinoma ACHN was increased. It weakened renal cell carcinoma ACHN in vitro movement. Chamber invasion assay showed that the number of cells was less in interference group than the con-trol group(P ﹤ 0. 05). Results showed that the interference weakens the invasive ability of tumor cells,compared with the control group. Conclusion:The role of CDC25B in renal cell carcinoma is as a proto - oncogene. The experiment indicated that CDC25B and malignant renal cell carcinoma were positively correlated. CDC25B may become a poten-tial renal cancer diagnostic marker and new indicator to determine the malignant degree.
