CAJ | 학술논문

目的：研究14-3-3β基因在胶质瘤细胞生长和增殖中的调控作用。方法：构建14-3-3β基因过表达质粒并设计14-3-3β基因和β- catenin 基因 siRNAs，分组转染人体正常神经星形胶质细胞系 SVGp12和胶质瘤细胞系 U87，噻唑蓝（MTT）法观察14-3-3β基因过表达/沉默和β- catenin 基因沉默对细胞生长和增殖的影响，并利用 qRT - PCR 定量分析受调控原癌基因的表达变化。结果：14-3-3β基因过表达的星形胶质细胞生长能力显著高于对照组（P ﹤0.05），而β- catenin 基因沉默的星形胶质细胞生长受到明显抑制（P ﹤0.05）。观察到14-3-3β基因或β- catenin 基因沉默的胶质瘤细胞生长能力也显著低于对照组细胞（P ﹤0.05）。结论：胶质瘤细胞的生长和增殖受到14-3-3β基因的调控并且其调控是通过β- catenin 基因来进行的，但其具体的信号调控机制需要进一步探讨。
목적：연구14-3-3β기인재효질류세포생장화증식중적조공작용。방법：구건14-3-3β기인과표체질립병설계14-3-3β기인화β- catenin 기인 siRNAs，분조전염인체정상신경성형효질세포계 SVGp12화효질류세포계 U87，새서람（MTT）법관찰14-3-3β기인과표체/침묵화β- catenin 기인침묵대세포생장화증식적영향，병이용 qRT - PCR 정량분석수조공원암기인적표체변화。결과：14-3-3β기인과표체적성형효질세포생장능력현저고우대조조（P ﹤0.05），이β- catenin 기인침묵적성형효질세포생장수도명현억제（P ﹤0.05）。관찰도14-3-3β기인혹β- catenin 기인침묵적효질류세포생장능력야현저저우대조조세포（P ﹤0.05）。결론：효질류세포적생장화증식수도14-3-3β기인적조공병차기조공시통과β- catenin 기인래진행적，단기구체적신호조공궤제수요진일보탐토。
To study the role of 14 - 3 - 3β on the regulation of the growth and proliferation of glioma cell. Methods:Recombinant plasmids containing 14 - 3 - 3β and small - interfering RNAs(siRNAs)targeting 14 - 3- 3β or β - catenin were transfected into the human normal astrocyte line SVGp12 and glioma cell line U87,respec-tively. The growth and proliferation of the transfected cells was analyzed by MTT assay. The expression of the oncogene was also analyzed by qRT - PCR. Results:Compared to the control,14 - 3 - 3β overexpression significantly promoted the growth of astrocyte cells(P ﹤ 0. 05). Meanwhile knockdown of β - catenin by siRNA blocked cell proliferation in-duced by overexpression of 14 - 3 - 3β(P ﹤ 0. 05). It was also observed that the proliferation of glioma cell was in-hibited by knockdown of either 14 - 3 - 3β or β - catenin by siRNA(P ﹤ 0. 05). Conclusion:The growth and prolif-eration of glioma cell was regulated by 14 - 3 - 3β,which was mediated by β - catenin. However,the precise underly-ing molecular mechanism needs to be further elucidated.