CAJ | 학술논문

以层状组装多层膜为基础，利用电化学方法精确可控的特点，构建了具有不同硬度的多层膜，并研究了该材料体系对细胞行为的影响规律。采用恒电流法，将聚吡咯( PPy )均匀复合进入聚赖氨酸/透明质酸( PLL/HA)多层膜内部，通过控制通电时间得到不同交联度的( PLL/HA) n@PPy多层膜。利用纳米压痕技术测试了多层膜在交联前后的硬度变化，并进一步研究了该多层膜体系对肿瘤细胞的黏附、增殖和迁移行为的影响。
이층상조장다층막위기출，이용전화학방법정학가공적특점，구건료구유불동경도적다층막，병연구료해재료체계대세포행위적영향규률。채용항전류법，장취필각( PPy )균균복합진입취뢰안산/투명질산( PLL/HA)다층막내부，통과공제통전시간득도불동교련도적( PLL/HA) n@PPy다층막。이용납미압흔기술측시료다층막재교련전후적경도변화，병진일보연구료해다층막체계대종류세포적점부、증식화천이행위적영향。
The layer-by-layer multilayers with controlled stiffness were fabricated through electrochemical methods. Polypyrrole( PPy) was synthesized by in situ electropolymerization of pyrrole within the poly ( L-ly-sine)/sodium hyaluronan(PLL/HA) multilayers by galvanostatic method to construct the crosslinked(PLL/HA) 10@ PPy film with interpenetrating network. Nano-indentation technology was used to characterize the stiffness of the multilayers. The adhesion, proliferation and migration of tumor cells were studied. The results showed that all these cellular behaviors was significantly affected by the ( PLL/HA)@PPy films with different stiffness. Our films could serve as surface modification technique for cell-based researches.