CAJ | 학술논문

采用离心超滤质谱分析技术(UF-UPLC/Q-TOF/MS),结合体外酶活性实验方法,对中药复方二妙丸提取物中的黄嘌呤氧化酶抑制剂进行了筛选.体外酶活性实验测得二妙丸水提液对黄嘌呤氧化酶的半数抑制浓度(IC50)为(0.218依0.0034) mg/mL,表明二妙丸具有较强的黄嘌呤氧化酶抑制活性.进一步采用离心超滤质谱技术对二妙丸水提物中潜在的黄嘌呤氧化酶抑制剂进行筛选,从中筛选并鉴定了9种具有潜在黄嘌呤氧化酶抑制活性的化合物,为开发黄嘌呤氧化酶抑制剂及阐明二妙丸治疗痛风和高尿酸血症的作用机制提供了一定的依据.
채용리심초려질보분석기술(UF-UPLC/Q-TOF/MS),결합체외매활성실험방법,대중약복방이묘환제취물중적황표령양화매억제제진행료사선.체외매활성실험측득이묘환수제액대황표령양화매적반수억제농도(IC50)위(0.218의0.0034) mg/mL,표명이묘환구유교강적황표령양화매억제활성.진일보채용리심초려질보기술대이묘환수제물중잠재적황표령양화매억제제진행사선,종중사선병감정료9충구유잠재황표령양화매억제활성적화합물,위개발황표령양화매억제제급천명이묘환치료통풍화고뇨산혈증적작용궤제제공료일정적의거.
An ultrafiltration UPLC/Q-TOF/MS assay and in vitro xanthine oxidase inhibition assay were com-bined to screen xanthine oxidase inhibitors in traditional Chinese herbal formulae Ermiaowan. The result of in vitro xanthine oxidase inhibition assay showed that Ermiaowan had a strong xanthine oxidase inhibitory activity, the IC50 value was (0.218±0.0034) mg/mL. Nine compounds were screened and identified as potential xan-thine oxidase inhibitors in Ermiaowan using ultrafiltration UPLC/Q-TOF/MS. This work provides a certain ba-sis for the development of xanthine oxidase inhibitors and the mechanism clarification of Ermiaowan to treat gout and hyperuricemia.