CAJ | 학술논문

应用链脲佐菌素( Streptozocin, STZ)制备糖尿病( Diabetes mellitus, DM)大鼠模型,采用离体的核磁共振磷谱(31 P Magnetic resonance spectroscopy, MRS)方法检测糖尿病大鼠脑组织的生化改变。全脑的31 P MRS谱图结果显示,STZ诱导1周后,磷酸单酯和磷酸二酯的含量无明显改变,表明糖尿病大鼠脑中并没有发生膜性结构的改变。二磷酸腺苷峰增高,磷酸肌酸( Phosphocreatine, PCr)和三磷酸腺苷( Adenosine triphosphate, ATP)含量无明显改变,但是PCr/ATP降低,说明PCr作为能量缓冲底物维持能量平衡。此外, pH值降低,表明在糖尿病大鼠脑中细胞内环境改变。当糖尿病发展到15周时,磷脂膜代谢和脑能量代谢紊乱。31 P MRS不仅能够无创性提供磷脂代谢情况及能量状况,还可以测得细胞内pH值等方面的生化信息,有助于理解糖尿病脑病的发病机制,并为临床的早期诊断和治疗提供理论依据。
응용련뇨좌균소( Streptozocin, STZ)제비당뇨병( Diabetes mellitus, DM)대서모형,채용리체적핵자공진린보(31 P Magnetic resonance spectroscopy, MRS)방법검측당뇨병대서뇌조직적생화개변。전뇌적31 P MRS보도결과현시,STZ유도1주후,린산단지화린산이지적함량무명현개변,표명당뇨병대서뇌중병몰유발생막성결구적개변。이린산선감봉증고,린산기산( Phosphocreatine, PCr)화삼린산선감( Adenosine triphosphate, ATP)함량무명현개변,단시PCr/ATP강저,설명PCr작위능량완충저물유지능량평형。차외, pH치강저,표명재당뇨병대서뇌중세포내배경개변。당당뇨병발전도15주시,린지막대사화뇌능량대사문란。31 P MRS불부능구무창성제공린지대사정황급능량상황,환가이측득세포내pH치등방면적생화신식,유조우리해당뇨병뇌병적발병궤제,병위림상적조기진단화치료제공이론의거。
Considerable attention has been directed toward studying the impact of diabetes on the central nervous system. The current study investigates the biochemical changes in the brain tissue of streptozotocin (STZ)-induced diabetic rat using 31P magnetic resonance spectroscopy (31P MRS). The 31P NMR spectra of the whole brain show no significant changes of phosphomonoesters and phosphodiesters levels one week after STZ induction, suggesting no apparent structural changes in cell membranes. The results identifies the increased level of adenosine diphosphate, negligible changes of phosphocreatine ( PCr ) and adenosine triphosphate ( ATP) , but the decreased ratio of PCr/ATP, indicating that PCr plays a role of balancing the energy. Moreover, the decreased pH value indicates the changes of the intracellular environment in STZ-diabetic brains in rats. After 15 weeks of STZ injection, the metabolism of phospholipid membrane and brain energy metabolism has been obviously disturbed. Our study successfully shows that 31 P MRS can not only study phospholipid and energy metabolism non-invasively, but also measure intracellular pH and other important biochemical information. All of these spectroscopic characterizations contribute significantly to the understanding of pathogenesis and evolution of diabetes, and provide theoretical basis for early diagnosis and clinical treatment in diabetes.