CAJ | 학술논문

基于胸腺嘧啶( T)-三聚氰胺-T特异性结构,建立了SYBR Green Ⅰ荧光均相检测三聚氰胺的方法。三聚氰胺不存在时,SYBR Green Ⅰ与聚T单链DNA结合较弱,荧光信号较弱；三聚氰胺存在时,聚T单链DNA通过T-三聚氰胺-T错配形成折叠结构。 SYBR Green Ⅰ嵌入双链DNA的双螺旋结构中,释放出强荧光信号。结果表明,三聚氰胺浓度在0.1~10μmol/L范围内呈良好的线性关系,检出限为35 nmol/L。本方法简单、快速、成本低、灵敏度高。
기우흉선밀정( T)-삼취청알-T특이성결구,건립료SYBR Green Ⅰ형광균상검측삼취청알적방법。삼취청알불존재시,SYBR Green Ⅰ여취T단련DNA결합교약,형광신호교약；삼취청알존재시,취T단련DNA통과T-삼취청알-T착배형성절첩결구。 SYBR Green Ⅰ감입쌍련DNA적쌍라선결구중,석방출강형광신호。결과표명,삼취청알농도재0.1~10μmol/L범위내정량호적선성관계,검출한위35 nmol/L。본방법간단、쾌속、성본저、령민도고。
A novel fluorescence homogeneous biosensing strategy was developed for simple, rapid and sensitive detection of melamine in milk by using the polythymine oligonucleotide T24 and SYBR Green Ⅰ. In the absence of melamine, the fluorescence of SYBR Green Ⅰ was weak. The interactions between the single strand oligonucleotide T24 and SYBR Green Ⅰ were weak. In contrast, the presence of melamine drove the formation of T-melamine-T folded structure and enabled the SYBR Green Ⅰ to intercalate into double-strand DNA, resulting in the enhancement of fluorescence intensity. The results revealed that the method allowed a sensitive, simple, and rapid assay of melamine with a linear response range from 0. 1 μmol/L to 10 μmol/L and a detection limit of 35 nmol/L.