CAJ | 학술논문

采用生物素-亲和素法在微悬臂梁传感芯片上固定维埃克斯、沙林适配子,建立了压阻式微悬臂梁适配子传感器检测维埃克斯、沙林及动力学分析方法。传感器对维埃克斯检测的线性范围为2~60μg/L,线性回归方程为△Ue=0.886C-1.039(n=5,R=0.984,p<0.001),检出限为2μg/L (S/N≥3)；对沙林检测的线性范围为10~60μg/L,线性回归方程为△Ue=0.716C-2.304(n=5,R =0.996,p<0.001),检出限为10μg/L (S/N≥3)。传感器具有良好的选择性和抗干扰能力,对毒剂类似物O-丁基甲基膦酰氯基本无响应。在此基础上,根据受体-配体结合特性与压阻式微悬臂梁传感器输出电压变化之间的关系,建立了传感器检测维埃克斯、沙林的反应动力学模型,根据拟合方程求出的传感器对不同浓度维埃克斯、沙林反应达到平衡时的响应电压(△Ue )、响应时间( t0)均与实测值非常接近。
채용생물소-친화소법재미현비량전감심편상고정유애극사、사림괄배자,건립료압조식미현비량괄배자전감기검측유애극사、사림급동역학분석방법。전감기대유애극사검측적선성범위위2~60μg/L,선성회귀방정위△Ue=0.886C-1.039(n=5,R=0.984,p<0.001),검출한위2μg/L (S/N≥3)；대사림검측적선성범위위10~60μg/L,선성회귀방정위△Ue=0.716C-2.304(n=5,R =0.996,p<0.001),검출한위10μg/L (S/N≥3)。전감기구유량호적선택성화항간우능력,대독제유사물O-정기갑기련선록기본무향응。재차기출상,근거수체-배체결합특성여압조식미현비량전감기수출전압변화지간적관계,건립료전감기검측유애극사、사림적반응동역학모형,근거의합방정구출적전감기대불동농도유애극사、사림반응체도평형시적향응전압(△Ue )、향응시간( t0)균여실측치비상접근。
A new method for O-ethyl S-[2-( diisopropylamino) ethyl] methylphosphonothiolate ( VX) , sarin detection and its kinetic analysis based on piezoresistive microcantilever aptasensor was developed, where VX, sarin aptamers were immobilized on the microcantilever surface by biotin-avidin binding system. A linear relationship between the response voltage and the concentration of VX in the range of 2-60μg/L was obtained. The linear regression equation was △Ue=0. 886C-1. 039 (n=5, R=0. 984, p<0. 001) and the detection limit was 2μg/L ( S/N≥3 ) . A linear relationship between the response voltage and the concentration of sarin in the range of 10-60 μg/L was obtained, the linear regression equation was △Ue=0. 716C-2. 304 ( n=5, R=0 . 996 , p<0 . 001 ) and the detection limit was 10 μg/L ( S/N≥3 ) . The sensor showed no response for O-butyl methylphosphonochloridate, a structural analog of VX and sarin, which indicated high specificity and good anti-interference ability. On this basis, a reaction kinetic model based on receptor-ligand binding and the relationship with output voltage change was established. Response voltage (△Ue ) and response time( t0 ) were obtained from the fitting equation on different concentrations of VX, sarin fitted well with the measured values.