CAJ | 학술논문

采用氮化钛( TiN)修饰平面微电极阵列( pMEA),对其性能进行改进,开展了离体神经电生理和神经递质电化学的检测研究。采用磁控溅射法在实验室自制微电极阵列上修饰具有纳米结构的TiN材料,修饰后的微电极阻抗降低近一个数量级,背景噪声基线降至±6μV,信噪比是修饰前的1.7倍。在SD大鼠离体脑片神经电生理信号检测中,信噪比可达10：1,能分离提取±12μV的微弱信号。神经递质多巴胺电化学信号检测下限达50 nmol/L(信噪比2：1),浓度在0.05~100μmol/L内与电流响应的线性相关系数为0.998。实验结果表明,在微电极表面修饰纳米TiN,降低了微电极阻抗,提高了信噪比,实现了对神经信息微弱信号的检测。
채용담화태( TiN)수식평면미전겁진렬( pMEA),대기성능진행개진,개전료리체신경전생리화신경체질전화학적검측연구。채용자공천사법재실험실자제미전겁진렬상수식구유납미결구적TiN재료,수식후적미전겁조항강저근일개수량급,배경조성기선강지±6μV,신조비시수식전적1.7배。재SD대서리체뇌편신경전생리신호검측중,신조비가체10：1,능분리제취±12μV적미약신호。신경체질다파알전화학신호검측하한체50 nmol/L(신조비2：1),농도재0.05~100μmol/L내여전류향응적선성상관계수위0.998。실험결과표명,재미전겁표면수식납미TiN,강저료미전겁조항,제고료신조비,실현료대신경신식미약신호적검측。
The nano-structure TiN was modified on the laboratory self-made planar microelectrode array pMEA by magnetron sputtering method. The performance of modified pMEA was investigated. Research on neuroelectrical and neurochemical recording was studied in vitro. The impedance of the modified pMEA was decreased almost one order of magnitude, and the background noise level was reduced to ±6 μV. In the same testing environment, the signal-to-noise ratio (SNR) of modified electrodes was 1. 7 times of bare electrodes. The SNR of neuroelectrical recording on the brain slice of SD rats reached 10:1 , and the weak signal such as ±12 μV was separated easily. For neuroelectrical recordings, the detection limit of dopamine ( DA) solution reached 50 nmol/L with the 2:1 (S/N). During the concentration range of 0. 05-100 μmol/L, the linearly correlation coefficient of the DA oxidation currents was 0 . 998 . The modification of nano-structure TiN on pMEA reduced pMEA impedance and background noise level, meanwhile the SNR was increased. The weak signals of neuroelectrical and neurochemical recording were successfully recorded.