CAJ | 학술논문

本试验旨在探讨低聚木糖（ XOS）是否影响凡纳滨对虾的生长性能、非特异性免疫力、抗氧化功能及抗对虾白斑综合征病毒（WSSV）能力。试验选用平均体重为（0．67±0．02）g的凡纳滨对虾幼虾800尾，随机分成4组（每组4个重复，每个重复50尾），分别投喂添加0、200、400和600 mg/kg XOS的饲料，并分别记作G0（对照）、G200、G400和G600组。试验期为6周。6周饲养试验结束后，进行WSSV感染试验。结果表明：饲料中添加200 mg/kg XOS能显著提高对虾的增重率、特定生长率、蛋白质效率和蛋白质沉积率（ P＜0．05），并显著降低饲料系数（ P＜0．05）。饲料中添加XOS能提高对虾的血细胞总数（ THC），且G400组显著高于对照组和G600组（ P＜0．05）。饲料中添加XOS能显著提高对虾血清酚氧化酶（ PO）和溶菌酶（ LZM）以及鳃LZM活力（ P＜0．05）。与对照组相比，G200组血清和肝胰腺超氧化物歧化酶（ SOD）和过氧化物酶（POD）活力及血清总抗氧化能力（T-AOC）显著升高（P＜0．05），各试验组肝胰腺T-AOC显著升高（ P＜0．05），G400组肝胰腺抗超氧阴离子自由基（ Anti-O-2·）活力显著升高（ P＜0．05），各试验组肝胰腺丙二醛（MDA）含量以及G400和G600组血清 MDA含量均显著降低（P＜0．05）。对虾感染WSSV 72 h 后，与对照组相比，各试验组对虾的累计死亡率均显著降低（ P＜0．05）， G200、G400、G600组的相对保护率分别为15．2％、18．2％和15．2％。由此得出，饲料中添加适量的XOS能提高凡纳滨对虾幼虾的生长性能、非特异性免疫力、抗氧化功能和抗 WSSV能力。
본시험지재탐토저취목당（ XOS）시부영향범납빈대하적생장성능、비특이성면역력、항양화공능급항대하백반종합정병독（WSSV）능력。시험선용평균체중위（0．67±0．02）g적범납빈대하유하800미，수궤분성4조（매조4개중복，매개중복50미），분별투위첨가0、200、400화600 mg/kg XOS적사료，병분별기작G0（대조）、G200、G400화G600조。시험기위6주。6주사양시험결속후，진행WSSV감염시험。결과표명：사료중첨가200 mg/kg XOS능현저제고대하적증중솔、특정생장솔、단백질효솔화단백질침적솔（ P＜0．05），병현저강저사료계수（ P＜0．05）。사료중첨가XOS능제고대하적혈세포총수（ THC），차G400조현저고우대조조화G600조（ P＜0．05）。사료중첨가XOS능현저제고대하혈청분양화매（ PO）화용균매（ LZM）이급새LZM활력（ P＜0．05）。여대조조상비，G200조혈청화간이선초양화물기화매（ SOD）화과양화물매（POD）활력급혈청총항양화능력（T-AOC）현저승고（P＜0．05），각시험조간이선T-AOC현저승고（ P＜0．05），G400조간이선항초양음리자자유기（ Anti-O-2·）활력현저승고（ P＜0．05），각시험조간이선병이철（MDA）함량이급G400화G600조혈청 MDA함량균현저강저（P＜0．05）。대하감염WSSV 72 h 후，여대조조상비，각시험조대하적루계사망솔균현저강저（ P＜0．05）， G200、G400、G600조적상대보호솔분별위15．2％、18．2％화15．2％。유차득출，사료중첨가괄량적XOS능제고범납빈대하유하적생장성능、비특이성면역력、항양화공능화항 WSSV능력。
This experiment was aimed to study whether xylo-oligosaccharides( XOS)supplementation could affect non-specific immunity,antioxidant function and anti-white spot syndrome virus( WSSV)capacity of ju-venile Litopenaeus vannamei. Eight hundred juvenile Litopenaeus vannamei with an initial body weight of (0.67±0.02)g were randomly assigned to 4 groups with 4 replicates per group and 50 shrimps per replicate. Shrimps in the 4 groups were fed diets supplemented with 0( G0 group,as control group ),200( G200 group),400( G400 group ) and 600 mg/kg( G600 group ) XOS for 6 weeks,respectively. After the 6 weeks’ feeding trial, shrimps were injected with WSSV. The results showed that the weight gain rate ( WGR),special gain rate( SGR),protein efficiency ratio( PER)and protein deposition rate( PDR)were significantly increased and the feed conversion ratio ( FCR ) was significantly decreased when adding 200 mg/kg in the diet( P<0.05). Dietary XOS could increase the total haemocyte count( THC),and that in G400 group was significantly higher than that in control group and G600 group( P<0.05). The activities of phenoloxidase( PO)and lysozyme( LZM)in serum and LZM in gill were significantly increased by adding XOS in the diet( P<0.05). Compared with control group,the activities of superoxide dismutase( SOD)and peroxidase( POD)in serum and hepatopancreas and serum total antioxidant capacity( T-AOC)in G200 group were significantly increased(P<0.05),the hepatopancreas T-AOC in experimental groups was significantly increased(P<0.05),and the activity of hepatopancreas anti-superoxide anion radical(Anti-O-2·)in G400 group was significantly increased(P<0.05),while the hepatopancreas malondialdehyde(MDA)content in G400 group and the serum MDA content in experimental groups were significantly reduced( P<0.05). After 72 h of WSSV injection,the cumulated mortality rate( CMR)in experimental groups was significantly de-creased compared with control group( P<0.05),and the relative percentage survival( RPS)in G200,G400 and G600 groups was 15.2%,18.2% and 15.2%,respectively. In conclusion,suitable amount of XOS in the diet can improve growth performance,non-specific immunity,antioxidant function and anti-WSSV capacity of juvenile Litopenaeus vannamei.