CAJ | 학술논문

本试验旨在研制一种适合工业生产用的配方成本低、生长效果好的酵母菌液体培养基。试验以当地8种农副产品为主要原料，采用均匀试验方案设计培养基组方，通过测定液态培养基发酵后酵母活菌数及酵母多糖含量来选择最佳碳源和氮源，并通过确定复合碳源、碳氮比及无机盐添加量最终确定液态培养基的组方。结果表明：酵母菌液体培养基组方为无水葡萄糖0．9％、糖蜜4．5％、麸皮3．6％、玉米干酒糟及其可溶物0．3％、棉籽粕0．275％、酵母浸膏0．075％和（NH3）2SO40．08％，无机盐为 KH2PO40．3％、MgSO40．03％和 CaCl20．03％，此时活菌数为2．38×108 CFU/mL，β-葡聚糖和甘露聚糖含量分别为651．6和210．8 mg/L。由此可见，酵母培养液既适用于工业生产中酵母菌的快速生长繁殖，又能节约原料成本。
본시험지재연제일충괄합공업생산용적배방성본저、생장효과호적효모균액체배양기。시험이당지8충농부산품위주요원료，채용균균시험방안설계배양기조방，통과측정액태배양기발효후효모활균수급효모다당함량래선택최가탄원화담원，병통과학정복합탄원、탄담비급무궤염첨가량최종학정액태배양기적조방。결과표명：효모균액체배양기조방위무수포도당0．9％、당밀4．5％、부피3．6％、옥미간주조급기가용물0．3％、면자박0．275％、효모침고0．075％화（NH3）2SO40．08％，무궤염위 KH2PO40．3％、MgSO40．03％화 CaCl20．03％，차시활균수위2．38×108 CFU/mL，β-포취당화감로취당함량분별위651．6화210．8 mg/L。유차가견，효모배양액기괄용우공업생산중효모균적쾌속생장번식，우능절약원료성본。
This experiment was conducted to develop a low-cost yeast liquid medium which was used in indus-trial production and had a good growth effect. In this study,eight kinds of local agricultural products were used as fermentation substrate. The components of the medium were designed by uniform design and the yeast viable count in the liquid medium after fermentation and the content of yeast polysaccharide was determined to select the best carbon source and nitrogen source. Then the composition of the liquid medium was determined by measuring the ratio of composite carbon source and composite nitrogen source and inorganic salts addition. The results showed that the composition of the yeast liquid culture included anhydrous glucose(0.9%),molasses (4. 5%), wheat bran ( 3. 6%), corn distillers dried grains with solubles ( 0. 3%), cottonseed meal (0.275%),yeast extract(0.075%)and(NH3)2SO4(0.08%).The inorganic salts included KH2PO4(0.3%), MgSO4(0.03%)and CaCl2(0.03%). The viable count was 2.38×108 CFU/mL and the contents of β-glucan and mannan were 651.6 and 210.8 mg/L. In conclusion,the yeast liquid medium is not only good for the yeast rapid growth and reproduction in the industrial production but also saves raw materials costs.