动物营养学报
動物營養學報
동물영양학보
ACTA ZOONUTRIMENTA SINICA
2014年
8期
2197-2208
,共12页
翟秋玲%张春晓%孙云章%王玲%宋凯%张璐
翟鞦玲%張春曉%孫雲章%王玲%宋凱%張璐
적추령%장춘효%손운장%왕령%송개%장로
三丁酸甘油酯%甘露寡糖%菊黄东方鲀%生长性能%体组成%肠道菌群%肠道结构
三丁痠甘油酯%甘露寡糖%菊黃東方鲀%生長性能%體組成%腸道菌群%腸道結構
삼정산감유지%감로과당%국황동방돈%생장성능%체조성%장도균군%장도결구
tributyrin%mannan-oligosaccharides%tawny puffer( Takifugu flavindus )%growth performance%body composition%intestinal microflora%intestinal structure
本试验旨在研究三丁酸甘油酯（ TB）与甘露寡糖（ MOS）对菊黄东方鲀生长性能、体组成及肠道健康指标的影响。以基础饲料作为对照，分别在基础饲料（干物质基础）中添加50 mg/kg TB、100 mg/kg TB、150 mg/kg TB、50 mg/kg TB＋250 mg/kg MOS、100 mg/kg TB＋250 mg/kg MOS，配制成5种试验饲料。试验选取初始体重为（15．99±0．15）g的菊黄东方鲀360尾，随机分为6组，每组3个重复，每个重复20尾。试验期为8周。结果表明：菊黄东方鲀的增重率、特定生长率、饲料效率和蛋白质效率随着 TB 添加量的增加先升高，在添加量为100 mg/kg时达到最大值，而后趋于平稳。与50 mg/kg TB 组相比，50 mg/kg TB＋250 mg/kg MOS组菊黄东方鲀的增重率、特定生长率、饲料效率和蛋白质效率显著升高（ P＜0．05）。此外，50 mg/kg TB＋250 mg/kg MOS组的增重率显著高于100 mg/kg TB＋250 mg/kg MOS组（ P＜0．05）。联合添加组的肝胰腺粗脂肪含量均显著高于对应添加量的单独添加组（ P＜0．05），肝糖原含量则均显著低于对应添加量的单独添加组（ P＜0．05），且以100 mg/kg TB＋250 mg/kg MOS组的肝糖原含量最低。50 mg/kg TB＋250 mg/kg MOS组的肠道蛋白酶活力显著高于其他各组（ P＜0．05）。50 mg/kg TB＋250 mg/kg MOS组与50 mg/kg TB组相比，血清总蛋白和葡萄糖含量均显著升高（P＜0．05），血清谷草转氨酶和谷丙转氨酶活力显著降低（P＜0．05）。对于肠道需氧异养细菌数量，50 mg/kg TB＋250 mg/kg MOS组与50 mg/kg TB组相比显著增加（ P＜0．05），100 mg/kg TB＋250 mg/kg MOS组与100 mg/kg TB组相比则显著下降（ P＜0．05）。与对照组相比，50 mg/kg TB＋250 mg/kg MOS组的肠道弧菌数量显著降低（ P＜0．05），肠道乳酸菌数量显著升高（ P＜0．05）。联合添加组的前肠和后肠绒毛高度和宽度均高于对应添加量的单独添加组，但二者间差异未达显著水平（ P＞0．05）。综上所述，饲料中添加50 mg/kg TB＋250 mg/kg MOS可维护菊黄东方鲀的肠道健康，增强肠道消化酶活力，提高饲料效率和生长性能。
本試驗旨在研究三丁痠甘油酯（ TB）與甘露寡糖（ MOS）對菊黃東方鲀生長性能、體組成及腸道健康指標的影響。以基礎飼料作為對照，分彆在基礎飼料（榦物質基礎）中添加50 mg/kg TB、100 mg/kg TB、150 mg/kg TB、50 mg/kg TB＋250 mg/kg MOS、100 mg/kg TB＋250 mg/kg MOS，配製成5種試驗飼料。試驗選取初始體重為（15．99±0．15）g的菊黃東方鲀360尾，隨機分為6組，每組3箇重複，每箇重複20尾。試驗期為8週。結果錶明：菊黃東方鲀的增重率、特定生長率、飼料效率和蛋白質效率隨著 TB 添加量的增加先升高，在添加量為100 mg/kg時達到最大值，而後趨于平穩。與50 mg/kg TB 組相比，50 mg/kg TB＋250 mg/kg MOS組菊黃東方鲀的增重率、特定生長率、飼料效率和蛋白質效率顯著升高（ P＜0．05）。此外，50 mg/kg TB＋250 mg/kg MOS組的增重率顯著高于100 mg/kg TB＋250 mg/kg MOS組（ P＜0．05）。聯閤添加組的肝胰腺粗脂肪含量均顯著高于對應添加量的單獨添加組（ P＜0．05），肝糖原含量則均顯著低于對應添加量的單獨添加組（ P＜0．05），且以100 mg/kg TB＋250 mg/kg MOS組的肝糖原含量最低。50 mg/kg TB＋250 mg/kg MOS組的腸道蛋白酶活力顯著高于其他各組（ P＜0．05）。50 mg/kg TB＋250 mg/kg MOS組與50 mg/kg TB組相比，血清總蛋白和葡萄糖含量均顯著升高（P＜0．05），血清穀草轉氨酶和穀丙轉氨酶活力顯著降低（P＜0．05）。對于腸道需氧異養細菌數量，50 mg/kg TB＋250 mg/kg MOS組與50 mg/kg TB組相比顯著增加（ P＜0．05），100 mg/kg TB＋250 mg/kg MOS組與100 mg/kg TB組相比則顯著下降（ P＜0．05）。與對照組相比，50 mg/kg TB＋250 mg/kg MOS組的腸道弧菌數量顯著降低（ P＜0．05），腸道乳痠菌數量顯著升高（ P＜0．05）。聯閤添加組的前腸和後腸絨毛高度和寬度均高于對應添加量的單獨添加組，但二者間差異未達顯著水平（ P＞0．05）。綜上所述，飼料中添加50 mg/kg TB＋250 mg/kg MOS可維護菊黃東方鲀的腸道健康，增彊腸道消化酶活力，提高飼料效率和生長性能。
본시험지재연구삼정산감유지（ TB）여감로과당（ MOS）대국황동방돈생장성능、체조성급장도건강지표적영향。이기출사료작위대조，분별재기출사료（간물질기출）중첨가50 mg/kg TB、100 mg/kg TB、150 mg/kg TB、50 mg/kg TB＋250 mg/kg MOS、100 mg/kg TB＋250 mg/kg MOS，배제성5충시험사료。시험선취초시체중위（15．99±0．15）g적국황동방돈360미，수궤분위6조，매조3개중복，매개중복20미。시험기위8주。결과표명：국황동방돈적증중솔、특정생장솔、사료효솔화단백질효솔수착 TB 첨가량적증가선승고，재첨가량위100 mg/kg시체도최대치，이후추우평은。여50 mg/kg TB 조상비，50 mg/kg TB＋250 mg/kg MOS조국황동방돈적증중솔、특정생장솔、사료효솔화단백질효솔현저승고（ P＜0．05）。차외，50 mg/kg TB＋250 mg/kg MOS조적증중솔현저고우100 mg/kg TB＋250 mg/kg MOS조（ P＜0．05）。연합첨가조적간이선조지방함량균현저고우대응첨가량적단독첨가조（ P＜0．05），간당원함량칙균현저저우대응첨가량적단독첨가조（ P＜0．05），차이100 mg/kg TB＋250 mg/kg MOS조적간당원함량최저。50 mg/kg TB＋250 mg/kg MOS조적장도단백매활력현저고우기타각조（ P＜0．05）。50 mg/kg TB＋250 mg/kg MOS조여50 mg/kg TB조상비，혈청총단백화포도당함량균현저승고（P＜0．05），혈청곡초전안매화곡병전안매활력현저강저（P＜0．05）。대우장도수양이양세균수량，50 mg/kg TB＋250 mg/kg MOS조여50 mg/kg TB조상비현저증가（ P＜0．05），100 mg/kg TB＋250 mg/kg MOS조여100 mg/kg TB조상비칙현저하강（ P＜0．05）。여대조조상비，50 mg/kg TB＋250 mg/kg MOS조적장도호균수량현저강저（ P＜0．05），장도유산균수량현저승고（ P＜0．05）。연합첨가조적전장화후장융모고도화관도균고우대응첨가량적단독첨가조，단이자간차이미체현저수평（ P＞0．05）。종상소술，사료중첨가50 mg/kg TB＋250 mg/kg MOS가유호국황동방돈적장도건강，증강장도소화매활력，제고사료효솔화생장성능。
This experiment was conducted to investigate the effects of tributyrin( TB)and mannan-oligosac-charides( MOS ) on growth performance,body composition and intestinal health indices of tawny puffer ( Takifugu flavindus). A basal diet was formulated as control,and 5 experimental diets were formulated by adding 50 mg/kg TB,100 mg/kg TB,150 mg/kg TB,50 mg/kg TB+250 mg/kg MOS and 100 mg/kg TB+250 mg/kg MOS in the basal diet,respectively. A total of 360 tawny puffer with an initial body weight of (15.99±0.15)g were randomly divided into 6 groups with 3 replicates per group and 20 fish per replicate. The experiment lasted for 8 weeks. The results showed that weight gain rate( WGR),specific growth rate( SGR), feed efficiency( FE)and protein efficiency ratio( PER)of tawny puffer were firstly increased with the TB supplemental level increasing,and the biggest values of them were all found in 100 mg/kg group,and then changed to be steady. Compared with 50 mg/kg TB group,the WGR,SGR,FE and PER of tawny puffer in 50 mg/kg TB+250 mg/kg MOS group were significantly increased( P<0. 05 ). Moreover,the WGR in 50 mg/kg TB+250 mg/kg MOS group was significantly higher than that in 100 mg/kg TB+250 mg/kg MOS group(P<0.05). Hepatopancreas crude lipid content in combined adding groups was significantly increased compared with single adding groups with the same TB supplemental level( P<0.05),while the hepaticglyco-gen content was significantly decreased( P<0. 05 ),and the lowest value was found in 100 mg/kg TB+250 mg/kg MOS group. Intestinal protease activity in 50 mg/kg TB+250 mg/kg MOS group was significantly higher than that in other groups( P<0.05). Compared with 50 mg/kg TB group,serum total protein and glu-cose contents were significantly increased( P<0.05),while serum glutamic-oxaloacetic transaminase and glu-tamic-pyruvic transaminase activities were significantly decreased( P<0.05). The number of intestinal aerobic marine heterotrophic bacteria in 50 mg/kg TB+250 mg/kg MOS group was significantly increased compared with 50 mg/kg TB group( P<0.05),while that in 100 mg/kg TB+250 mg/kg MOS group was significantly decreased compared with 100 mg/kg TB group( P<0.05). Compared with control group,the number of intes-tinal Vibrio spp. in 50 mg/kg TB+250 mg/kg MOS group was significantly increased( P<0.05),while the number of intestinal Lactobacillus was significantly decreased( P<0.05). Villus height and width of proximal and distal intestines in combined adding groups were all higher than those in single adding groups with the same TB supplemental level,but the differences were not significant( P>0. 05 ). The results indicate that adding 50 mg/kg TB+250 mg/kg MOS in the diet can maintenance the intestinal health,strength the intestinal diges-tive activities and increase the growth performance of tawny puffer.
