CAJ | 학술논문

本试验旨在研究母猪饲粮中添加精氨酸（ Arg）对子宫内发育迟缓（ IUGR）仔猪肠道免疫细胞数量的影响。试验选择20头妊娠30 d 的母猪（长白×大白），随机分为2组（每组10头），分别饲喂基础饲粮和添加1％ Arg的基础饲粮至母猪生产。从饲喂基础饲粮的母猪所产的仔猪中选8头正常初生重母猪和8头 IUGR 母猪，分别为对照组和 IUGR 组；从饲喂添加1％Arg的基础饲粮的母猪所产仔猪中选择8头IUGR母猪，为Arg＋IUGR组，各组仔猪随母猪哺乳。于仔猪7和21日龄，每组随机选取4头仔猪屠宰，检测空肠及回肠黏膜免疫细胞数量。结果表明：Arg＋IUGR组仔猪8～21日龄及1～21日龄平均日增重高于IUGR组（ P＞0．05）；IUGR组仔猪7日龄小肠杯状细胞数量和21日龄回肠杯状细胞数量均显著低于对照组（ P＜0．05），Arg＋IUGR组仔猪7日龄空肠肥大细胞和回肠杯状细胞数量极显著高于 IUGR组（ P＜0．01），回肠肥大细胞和空肠杯状细胞数量也有所提高（ P＞0．05）；Arg＋IUGR组仔猪21日龄空肠肥大细胞和杯状细胞数量及回肠杯状细胞数量均显著高于IUGR组（ P＜0．05）。综上所述，母猪饲粮中添加Arg可提高IUGR仔猪平均日增重，增加后代IUGR仔猪肠道免疫细胞数量，增强仔猪肠道免疫力。
본시험지재연구모저사량중첨가정안산（ Arg）대자궁내발육지완（ IUGR）자저장도면역세포수량적영향。시험선택20두임신30 d 적모저（장백×대백），수궤분위2조（매조10두），분별사위기출사량화첨가1％ Arg적기출사량지모저생산。종사위기출사량적모저소산적자저중선8두정상초생중모저화8두 IUGR 모저，분별위대조조화 IUGR 조；종사위첨가1％Arg적기출사량적모저소산자저중선택8두IUGR모저，위Arg＋IUGR조，각조자저수모저포유。우자저7화21일령，매조수궤선취4두자저도재，검측공장급회장점막면역세포수량。결과표명：Arg＋IUGR조자저8～21일령급1～21일령평균일증중고우IUGR조（ P＞0．05）；IUGR조자저7일령소장배상세포수량화21일령회장배상세포수량균현저저우대조조（ P＜0．05），Arg＋IUGR조자저7일령공장비대세포화회장배상세포수량겁현저고우 IUGR조（ P＜0．01），회장비대세포화공장배상세포수량야유소제고（ P＞0．05）；Arg＋IUGR조자저21일령공장비대세포화배상세포수량급회장배상세포수량균현저고우IUGR조（ P＜0．05）。종상소술，모저사량중첨가Arg가제고IUGR자저평균일증중，증가후대IUGR자저장도면역세포수량，증강자저장도면역력。
This study was conducted to study the effects of sow diet supplemented with arginine( Arg)on the number of intestinal mucosal immune cells of offspring piglets. On day 30 of gestation,twenty pregnant sows (Landrace×White)were randomly divided into 2 groups(10 pregnant sows per group),receiving a basal diet and the basal diet supplemented with 1% Arg,respectively. Eight neonatal piglets with a normal birth weight ( control group)and 8 piglets with intrauterine growth retardation( IUGR group)chosen from the sows fed the basal diet,respectively,and 8 IUGR piglets( Arg+IUGR group)chosen from the sows fed the basal diet sup-plemented with 1% Arg. Four piglets of each group were slaughtered to determine the number of immune cells in jejunum and ileum mucosae at 7 and 21 days of age. The results showed that compared with IUGR group, the average daily gain of piglets in Arg+IUGR group was increased at 8 to 21 days of age and 1 to 21 days of age( P>0.05). Compared with IUGR group,the number of mast cells in jejunum and goblet cells in ileum of piglets in Arg+IUGR group were significantly increased at 7 days of age( P<0.01),and the number of mast cells in ileum and goblet cells in jejunum were increased( P>0.05). At 21 days of age,the number of mast cells and goblet cells in jejunum were significantly increased( P<0.05),as well as the number of goblet cells in ileum( P<0.05). The results indicate that the pregnant sow diet supplemented with Arg can increase the av-erage daily gain and the number of immune cells in intestinal mucosa of IUGR piglets,and enhance intestinal immunity of offspring.