CAJ | 학술논문

采用微生物直接转化药材的方法,将栀子中的京尼平苷转化为京尼平,无需糖苷酶和京尼平苷的制备.在培养温度为30°C, pH 6.1以及栀子载量为80 g/L的条件下,48 h京尼平苷的转化率为97.8%.转化后的京尼平通过XAD-16N大孔树脂偶联硅胶层析的方法,制备得到纯度大于95%的京尼平,收率为62.3%.在催化、转化机制研究中,从哈茨木霉CGMCC2979的发酵液中分离得到了分子量为74.4 kDa的京尼平苷β-葡萄糖苷酶,该酶最优催化条件为50°C和pH 4.0-5.0.Km和Vmax分别为3.6 mmol/L和775μmol/ h/mg蛋白.本文提供了一种简便、高效制备京尼平的新方法.
채용미생물직접전화약재적방법,장치자중적경니평감전화위경니평,무수당감매화경니평감적제비.재배양온도위30°C, pH 6.1이급치자재량위80 g/L적조건하,48 h경니평감적전화솔위97.8%.전화후적경니평통과XAD-16N대공수지우련규효층석적방법,제비득도순도대우95%적경니평,수솔위62.3%.재최화、전화궤제연구중,종합자목매CGMCC2979적발효액중분리득도료분자량위74.4 kDa적경니평감β-포도당감매,해매최우최화조건위50°C화pH 4.0-5.0.Km화Vmax분별위3.6 mmol/L화775μmol/ h/mg단백.본문제공료일충간편、고효제비경니평적신방법.
Trichoderma harzianum (T. harzianum) CGMCC 2979 was used to transform the geniposide in Gar-denia jasminoides (G. jasminoides) to genipin, dispensing the use of purified enzyme and the extrac-tion of geniposide from the raw material. At 30 °C, pH 6.1, and an initial G. jasminoides concentration of 80 g dried fruit per liter of medium, the geniposide-to-genipin conversion rate reached 97.8%after 48 h of fermentation. The genipin was purified from the fermentation broth by a combined method of XAD-16N-resin and silica-gel chromatography, yielding a total recovery of 62.3%. A 74.4-kDa geniposide-β-glucosidase implicated in the transformation of geniposide to genipin was purified from T. harzianum CGMCC 2979. It had optimum activity at 50 °C and pH 4.0-5.0. The Km and Vmax of the enzyme for geniposide were 3.6 mmol/L and 775μmol/h/mg protein, respectively. The simple, direct, and efficient biotransformation of geniposide in G. jasminoide to genipin by T. harzianum CGMCC 2979 that is described in this study could represent an alternative and effective method for producing genipin.