中国老年学杂志
中國老年學雜誌
중국노년학잡지
CHINESE JOURNAL OF GERONTOLOGY
2014年
13期
3686-3688
,共3页
张健昌%,黄家园%,黄河%周嘉雯%暴珑%谢敏%朱铭%张艳翎%黎黎%吴科锋%李文德%黄韧
張健昌%,黃傢園%,黃河%週嘉雯%暴瓏%謝敏%硃銘%張豔翎%黎黎%吳科鋒%李文德%黃韌
장건창%,황가완%,황하%주가문%폭롱%사민%주명%장염령%려려%오과봉%리문덕%황인
消旋卡多曲%β-淀粉样蛋白%脑啡肽酶%阿尔茨海默病
消鏇卡多麯%β-澱粉樣蛋白%腦啡肽酶%阿爾茨海默病
소선잡다곡%β-정분양단백%뇌배태매%아이자해묵병
Racecadotril%Beta-amyliod%Neprilysin%Alzheimer′s disease
目的：探讨消旋卡多曲对老年大鼠脑内β-淀粉样蛋白的水平的影响。方法30只老年 SD大鼠随机分为空白对照组，消旋卡多曲高剂量组，消旋卡多曲低剂量组。消旋卡多曲高、低剂量组，连续4 w，1次/d，给予不同剂量消旋卡多曲灌胃，空白对照组灌以等量20％ PEG400溶液。灌胃结束后，酶联免疫吸附法（ ELISA）测定大脑及外周血中Aβ1～40、Aβ1～42和脑啡肽酶（ NEP）的含量，病理检查观察大脑海马区的病理改变情况。结果 ELISA结果显示，与空白对照组相比，消旋卡多曲各剂量组脑组织Aβ1～40、Aβ1～42水平较对照组显著升高（P＜0.05），NEP水平有下降的趋势，仅高剂量组差异有显著性（ P＜0.05）；空白对照组与消旋卡多曲各剂量组在外周血液中 Aβ1～40、Aβ1～42和 NEP 的含量均无明显差异（ P＞0.05）；病理检查发现消旋卡多曲各剂量组大脑海马CA3区及齿状回神经元较空白对照组明显减少。结论超量应用消旋卡多曲可促使脑组织中Aβ水平升高，有增加老年大鼠AD发病风险的可能。
目的：探討消鏇卡多麯對老年大鼠腦內β-澱粉樣蛋白的水平的影響。方法30隻老年 SD大鼠隨機分為空白對照組，消鏇卡多麯高劑量組，消鏇卡多麯低劑量組。消鏇卡多麯高、低劑量組，連續4 w，1次/d，給予不同劑量消鏇卡多麯灌胃，空白對照組灌以等量20％ PEG400溶液。灌胃結束後，酶聯免疫吸附法（ ELISA）測定大腦及外週血中Aβ1～40、Aβ1～42和腦啡肽酶（ NEP）的含量，病理檢查觀察大腦海馬區的病理改變情況。結果 ELISA結果顯示，與空白對照組相比，消鏇卡多麯各劑量組腦組織Aβ1～40、Aβ1～42水平較對照組顯著升高（P＜0.05），NEP水平有下降的趨勢，僅高劑量組差異有顯著性（ P＜0.05）；空白對照組與消鏇卡多麯各劑量組在外週血液中 Aβ1～40、Aβ1～42和 NEP 的含量均無明顯差異（ P＞0.05）；病理檢查髮現消鏇卡多麯各劑量組大腦海馬CA3區及齒狀迴神經元較空白對照組明顯減少。結論超量應用消鏇卡多麯可促使腦組織中Aβ水平升高，有增加老年大鼠AD髮病風險的可能。
목적：탐토소선잡다곡대노년대서뇌내β-정분양단백적수평적영향。방법30지노년 SD대서수궤분위공백대조조，소선잡다곡고제량조，소선잡다곡저제량조。소선잡다곡고、저제량조，련속4 w，1차/d，급여불동제량소선잡다곡관위，공백대조조관이등량20％ PEG400용액。관위결속후，매련면역흡부법（ ELISA）측정대뇌급외주혈중Aβ1～40、Aβ1～42화뇌배태매（ NEP）적함량，병리검사관찰대뇌해마구적병리개변정황。결과 ELISA결과현시，여공백대조조상비，소선잡다곡각제량조뇌조직Aβ1～40、Aβ1～42수평교대조조현저승고（P＜0.05），NEP수평유하강적추세，부고제량조차이유현저성（ P＜0.05）；공백대조조여소선잡다곡각제량조재외주혈액중 Aβ1～40、Aβ1～42화 NEP 적함량균무명현차이（ P＞0.05）；병리검사발현소선잡다곡각제량조대뇌해마CA3구급치상회신경원교공백대조조명현감소。결론초량응용소선잡다곡가촉사뇌조직중Aβ수평승고，유증가노년대서AD발병풍험적가능。
Objective To evaluate the risk of Alzheimer′s disease which be used Racecadotril to effect the level of beta-amyliod in the brain of aged rats.Methods 30 mices were randomly divided into Racecadotril high , low dose treatment and control groups.Rats were given gastric perfusion of Racecadotril in Racecadotril high , low dose treatment groups , and were perfused to same volume 20%PEG400 in control group.Levels of Aβ1~40 ,Aβ1~42 , NEP in brain and peripheral blood were analyzed by ELISA and the effect of Racecadotril on mice brain was investigated by H&E staining.Results Aβ1~40 ,Aβ1~42 levels were significantly increased in the brain of low and high dose of Racecadotril groups compared with the control group ( P<0.05 ).The level of NEP had decreasing tendency , there was significant difference between high dose of Racecadotril group and control group ( P<0.05 ).But all rats had minor changes of peripheral blood.H&E staining showed neuronal loss and a proliferation of microglia were detected in hippocampal formation.Conclusions Racecadotril overdose could in-crease the risk of Alzheimer′disease in aged rats by upregulating Aβ1~40 , Aβ1~42 levels of brain tissue.
