CAJ | 학술논문

目的：探讨沉默血管内皮生长因子（ VEGF-C）基因对人非小细胞肺癌（ NSCLC） A549细胞增殖和侵袭能力的影响。方法构建靶向VEGF-C基因的重组慢病毒Lv-siRNA-VEGF-C，将其感染A549细胞，建立VEGF-C基因低表达的A549细胞。 Western印迹检测Lv-siRNA-VEGF-C细胞组、空载体Lv-Ctrl 对照组及空白A549细胞中VEGF-C蛋白的表达噻唑蓝（ MTT）法、Transwell侵袭实验和裸鼠移植瘤模型分别检测各组细胞的增殖和迁移能力。结果与对照相比，VEGF-C基因低表达组细胞中VEGF-C蛋白的表达显著降低，细胞体内、体外增殖活性也明显下降，细胞侵袭能力亦显著降低。结论成功构建的慢病毒介导的针对VEGF-C的干扰载体可有效抑制A549细胞表达 VEGF-C 蛋白表达，沉默 VEGF-C能抑制人NSCLC A549细胞的增殖和侵袭能力。
목적：탐토침묵혈관내피생장인자（ VEGF-C）기인대인비소세포폐암（ NSCLC） A549세포증식화침습능력적영향。방법구건파향VEGF-C기인적중조만병독Lv-siRNA-VEGF-C，장기감염A549세포，건립VEGF-C기인저표체적A549세포。 Western인적검측Lv-siRNA-VEGF-C세포조、공재체Lv-Ctrl 대조조급공백A549세포중VEGF-C단백적표체새서람（ MTT）법、Transwell침습실험화라서이식류모형분별검측각조세포적증식화천이능력。결과여대조상비，VEGF-C기인저표체조세포중VEGF-C단백적표체현저강저，세포체내、체외증식활성야명현하강，세포침습능력역현저강저。결론성공구건적만병독개도적침대VEGF-C적간우재체가유효억제A549세포표체 VEGF-C 단백표체，침묵 VEGF-C능억제인NSCLC A549세포적증식화침습능력。
Objective To explore the effect of silencing vascular endothelial growth factor ( VEGF)-C gene expression on prolifera-tion and invasion of non-small cell lung cancer (NSCLC) A549 cell.Methods The recombinant lentivirus Lv-siRNA-VEGF-C targeting VEGF-C gene was constructed and then was transfected into A 549 cells to establish VEGF-C low expressed cells .The expression level of VEGF-C protein in A549 cells was examined by Western blot in Lv-siRNA-VEGF-C, Lv-Con control and blank control groups .The prolifera-tion and invasion of A549 cells were detected by MTT, transwell analysis and human lung cancer xenograft respectively .Results Compared with that of control group ,the expression of VEGF-C protein in A549 cells was significantly lower in Lv-siRNA-VEGF-C A549 cells, and the proliferation activity in vivo and in vitro of low VEGF-C expressed cells were also decreased significantly .Simutanously,the invasion of low expressed VEGF-C A549 cells was significantly increased .Conclusions Lentvirus-mediated silencing VEGF-C gene could inhibit the prolif-eration, migration and invasion of human non-small cell lung cancer A549 cells.