空军医学杂志
空軍醫學雜誌
공군의학잡지
MEDICAL JOURNAL OF AIR FORCE
2014年
2期
83-85,89
,共4页
右美托咪定%神经病理性痛%神经元型一氧化氮合酶
右美託咪定%神經病理性痛%神經元型一氧化氮閤酶
우미탁미정%신경병이성통%신경원형일양화담합매
Dexmedetomidine%Neuropathic pain%Neuronal nitric oxide synthase
目的：观察连续腹腔注射右美托咪定（dexmedetomidine，DEX）对神经病理性痛大鼠热痛阈改变和脊髓中央管周围灰质神经元型一氧化氮合酶（neuronal nitric oxide synthase，nNOS）表达的影响。方法健康成年雄性SD大鼠36只，随机分为3组，分别为假手术组（Sham组）、坐骨神经慢性缩窄性损伤组（chronic constrictive injury，CCI组）和CCI+右美托咪定组（DEX组）。采用坐骨神经慢性缩窄性损伤的方法制备慢性神经病理性痛模型；热缩足反射潜伏期（thermal withdrawal latency，TWL）测定大鼠热痛阈的变化作为行为学观察指标；采用免疫组化的方法观察大鼠脊髓中央管周围灰质nNOS表达的变化。结果与Sham组相比，CCI组和DEX组大鼠术后各时间点热痛阈值均降低（P＜0.05），脊髓中央管周围灰质nNOS阳性神经元数目显著增加（P＜0.05）；与CCI组相比，连续腹腔注射DEX 7、14 d大鼠热痛阈值升高（P＜0.05），脊髓中央管周围灰质nNOS阳性神经元数目显著减少（P＜0.05）。结论脊髓中央管周围灰质nNOS的表达下调参与了DEX对神经病理性痛的治疗作用。
目的：觀察連續腹腔註射右美託咪定（dexmedetomidine，DEX）對神經病理性痛大鼠熱痛閾改變和脊髓中央管週圍灰質神經元型一氧化氮閤酶（neuronal nitric oxide synthase，nNOS）錶達的影響。方法健康成年雄性SD大鼠36隻，隨機分為3組，分彆為假手術組（Sham組）、坐骨神經慢性縮窄性損傷組（chronic constrictive injury，CCI組）和CCI+右美託咪定組（DEX組）。採用坐骨神經慢性縮窄性損傷的方法製備慢性神經病理性痛模型；熱縮足反射潛伏期（thermal withdrawal latency，TWL）測定大鼠熱痛閾的變化作為行為學觀察指標；採用免疫組化的方法觀察大鼠脊髓中央管週圍灰質nNOS錶達的變化。結果與Sham組相比，CCI組和DEX組大鼠術後各時間點熱痛閾值均降低（P＜0.05），脊髓中央管週圍灰質nNOS暘性神經元數目顯著增加（P＜0.05）；與CCI組相比，連續腹腔註射DEX 7、14 d大鼠熱痛閾值升高（P＜0.05），脊髓中央管週圍灰質nNOS暘性神經元數目顯著減少（P＜0.05）。結論脊髓中央管週圍灰質nNOS的錶達下調參與瞭DEX對神經病理性痛的治療作用。
목적：관찰련속복강주사우미탁미정（dexmedetomidine，DEX）대신경병이성통대서열통역개변화척수중앙관주위회질신경원형일양화담합매（neuronal nitric oxide synthase，nNOS）표체적영향。방법건강성년웅성SD대서36지，수궤분위3조，분별위가수술조（Sham조）、좌골신경만성축착성손상조（chronic constrictive injury，CCI조）화CCI+우미탁미정조（DEX조）。채용좌골신경만성축착성손상적방법제비만성신경병이성통모형；열축족반사잠복기（thermal withdrawal latency，TWL）측정대서열통역적변화작위행위학관찰지표；채용면역조화적방법관찰대서척수중앙관주위회질nNOS표체적변화。결과여Sham조상비，CCI조화DEX조대서술후각시간점열통역치균강저（P＜0.05），척수중앙관주위회질nNOS양성신경원수목현저증가（P＜0.05）；여CCI조상비，련속복강주사DEX 7、14 d대서열통역치승고（P＜0.05），척수중앙관주위회질nNOS양성신경원수목현저감소（P＜0.05）。결론척수중앙관주위회질nNOS적표체하조삼여료DEX대신경병이성통적치료작용。
Objective To investigate the effect of dexmedetomidine on the pain threshold of hit and the expression of neuronal nitric oxide synthase (nNOS)in the gray matter around the central canal in the spinal cord in a rat model of chronic neuropathic pain. Methods Thirty-six male SD rats were randomly assigned into 3 groups: sham operation group (group Sham), chronic constrictive injury operation group (group CCI), CCI+dexmedetomidine group (group DEX). Chronic neuropathic pain was induced by chronic constrictive injury. The behavioral studies of rats were scored and thethermal withdrawal latency (TWL) was measured. The expression of nNOS in the gray matter around the central canal in the spinal cord was observed by immunohistochemistry. Results Compared with group Sham, TWL was significantly decreased and the expression of nNOS was up-regulated after operation in group CCI and group DEX(P<0.05). Compared with group CCI, TWL was significantly increased and the expression of nNOS was significantly down-regulated on day 7 and 14 after operation in group DEX(P<0.05). Conclusion Down-regulated nNOS in the gray matter around the central canal in the spinal cord is involved in the attenuation of neuropathic pain by DEX.
