中西医结合研究
中西醫結閤研究
중서의결합연구
RESEARCH OF INTEGRATED TRADITIONAL CHINESE AND WESTERN MEDICINE
2014年
3期
133-136
,共4页
牛文民%刘智斌%杨晓航%王渊
牛文民%劉智斌%楊曉航%王淵
우문민%류지빈%양효항%왕연
阿茨海默病%学习记忆%蛋白激酶 A%嗅三针
阿茨海默病%學習記憶%蛋白激酶 A%嗅三針
아자해묵병%학습기억%단백격매 A%후삼침
Alzheimer’s disease%learning-memory%protein kinase A%Xiusanzhen
目的：通过观察嗅三针疗法对阿茨海默病（Alzheimer’s disease ，AD）大鼠的学习记忆能力与海马组织蛋白激酶A （protein kinase A ，PKA ）活性的影响，探讨嗅三针疗法治疗 AD 的作用机制。方法将成年SD雄性大鼠50只随机分为正常对照组、AD模型组、AD＋嗅神经切断模型组、嗅三针组和嗅三针＋嗅神经切断组，每组10只。制作 A D大鼠和 A D嗅神经切断大鼠模型，嗅三针组和嗅三针＋嗅神经切断组均进行嗅三针治疗，通过嗅三针治疗，测试大鼠水迷宫学习记忆能力，并采用 ELISA 法测定海马组织 PKA活性。结果各组大鼠6 d平均逃避潜伏期和平均游泳路程比较，正常对照组、嗅三针组与嗅三针＋嗅神经切断组均显著短于 AD 模型组（P＜0.01，P＜0.05）；嗅三针组短于嗅三针＋嗅神经切断组（P＜0.05）；AD模型组与 AD ＋嗅神经切断组相比较，其差异无统计学意义（P＞0.05）。各组大鼠海马组织 PKA 活性比较，正常对照组、嗅三针组和嗅三针＋嗅神经切断组均明显高于 AD 模型组（P＜0.01），嗅三针组高于嗅三针＋嗅神经切断组（P＜0.05），AD模型组与AD＋嗅神经切断组相比较，其差异无统计学意义（P＞0.05）。结论嗅三针能够显著增强 AD 大鼠学习记忆功能、并且能提高海马组织 PK A 活性，其治疗效应的发挥依赖于嗅觉传导通路的完整性。
目的：通過觀察嗅三針療法對阿茨海默病（Alzheimer’s disease ，AD）大鼠的學習記憶能力與海馬組織蛋白激酶A （protein kinase A ，PKA ）活性的影響，探討嗅三針療法治療 AD 的作用機製。方法將成年SD雄性大鼠50隻隨機分為正常對照組、AD模型組、AD＋嗅神經切斷模型組、嗅三針組和嗅三針＋嗅神經切斷組，每組10隻。製作 A D大鼠和 A D嗅神經切斷大鼠模型，嗅三針組和嗅三針＋嗅神經切斷組均進行嗅三針治療，通過嗅三針治療，測試大鼠水迷宮學習記憶能力，併採用 ELISA 法測定海馬組織 PKA活性。結果各組大鼠6 d平均逃避潛伏期和平均遊泳路程比較，正常對照組、嗅三針組與嗅三針＋嗅神經切斷組均顯著短于 AD 模型組（P＜0.01，P＜0.05）；嗅三針組短于嗅三針＋嗅神經切斷組（P＜0.05）；AD模型組與 AD ＋嗅神經切斷組相比較，其差異無統計學意義（P＞0.05）。各組大鼠海馬組織 PKA 活性比較，正常對照組、嗅三針組和嗅三針＋嗅神經切斷組均明顯高于 AD 模型組（P＜0.01），嗅三針組高于嗅三針＋嗅神經切斷組（P＜0.05），AD模型組與AD＋嗅神經切斷組相比較，其差異無統計學意義（P＞0.05）。結論嗅三針能夠顯著增彊 AD 大鼠學習記憶功能、併且能提高海馬組織 PK A 活性，其治療效應的髮揮依賴于嗅覺傳導通路的完整性。
목적：통과관찰후삼침요법대아자해묵병（Alzheimer’s disease ，AD）대서적학습기억능력여해마조직단백격매A （protein kinase A ，PKA ）활성적영향，탐토후삼침요법치료 AD 적작용궤제。방법장성년SD웅성대서50지수궤분위정상대조조、AD모형조、AD＋후신경절단모형조、후삼침조화후삼침＋후신경절단조，매조10지。제작 A D대서화 A D후신경절단대서모형，후삼침조화후삼침＋후신경절단조균진행후삼침치료，통과후삼침치료，측시대서수미궁학습기억능력，병채용 ELISA 법측정해마조직 PKA활성。결과각조대서6 d평균도피잠복기화평균유영로정비교，정상대조조、후삼침조여후삼침＋후신경절단조균현저단우 AD 모형조（P＜0.01，P＜0.05）；후삼침조단우후삼침＋후신경절단조（P＜0.05）；AD모형조여 AD ＋후신경절단조상비교，기차이무통계학의의（P＞0.05）。각조대서해마조직 PKA 활성비교，정상대조조、후삼침조화후삼침＋후신경절단조균명현고우 AD 모형조（P＜0.01），후삼침조고우후삼침＋후신경절단조（P＜0.05），AD모형조여AD＋후신경절단조상비교，기차이무통계학의의（P＞0.05）。결론후삼침능구현저증강 AD 대서학습기억공능、병차능제고해마조직 PK A 활성，기치료효응적발휘의뢰우후각전도통로적완정성。
Objective To explore the therapeutical mechanism of Xiusanzhen acupuncture treating Alzheimer ’s disease(AD)through observing effects of Xiusanzhen acupuncture on learning-memory abilities and protein kinase A(PKA)activity of hippocampus in AD rats.Methods The 50 SD rats were randomly divided into normal control , AD model ,AD + severed olfactory nerve model ,Xiusanzhen ,and Xiusanzhen + severed olfactory nerve model group.AD models were established by injection of Aβ1~40 and severed olfactory nerve.Morris maze tests were conducted for valuating the learning and memory abilities.The PKA activity of hippocampus were detected by ELISA.Results In comparison with AD model group ,the average escape latency and travel distance shortened sig-nificantly in normal control ,Xiusanzhen ,and Xiusanzhen + severed olfactory nerve model group ( P< 0.01 ,P<0.05).In comparison with Xiusanzhen+ severed olfactory nerve model group ,the average escape latency and travel distance shortened in Xiusanzhen groups (P< 0.05).No significant difference was found between AD model and AD+ severed olfactory nerve model(P>0.05).The PKA activity of hippocampus in normal control ,Xiusanzhen , and Xiusanzhen+severed olfactory nerve model group was higher than that of the control AD model group (P<0.01).The PKA activity of hippocampus in Xiusanzhen group was higher than that of Xiusanzhen +severed olfac-tory nerve model group(P<0.05).No significant differences were found between AD model and AD plus severed olfactory nerve model groups (P> 0.05).Conclusion The effect of Xiusanzhen acupuncture could increased the learning memory abilities and PKA activity of hippocampus in AD rats.Its therapeutical effects depended on the entirety of olfactory pathway.
