山东医学高等专科学校学报
山東醫學高等專科學校學報
산동의학고등전과학교학보
JOURNAL OF SHANDONG MEDICAL COLLEGE
2014年
3期
223-225
,共3页
系列特异性抗原%急性髓性白血病%流式细胞术%免疫表型分型
繫列特異性抗原%急性髓性白血病%流式細胞術%免疫錶型分型
계렬특이성항원%급성수성백혈병%류식세포술%면역표형분형
Serial specific antigens%Acute myeloid leukaemia%Flow cytometry%Immunophenotype analysis
目的：探讨细胞系列特异性抗原在急性髓性白血病（AML）中的表达及临床意义。方法采用CD45/SSC双参数设门流式细胞术检测120例A M L患者幼稚细胞表面及胞浆抗原表达。结果在A M L中细胞膜抗原CD13、CD33和CD117表达最常见，分别为95．0％、95．0％和79．2％， CD117特异性高于CD13和CD33（ P ＜0．01）；胞浆抗原cM PO敏感性和特异性与CD117、胞浆抗原CD19敏感性与cCD79a比较差异均无统计学意义（ P ＞20．05），但cCD79a的特异性高于CD19（ P ＜0．01）。结论多参数流式细胞数检测细胞系列特异性胞浆抗原表达是诊断急性髓性白血病的一项可靠技术。
目的：探討細胞繫列特異性抗原在急性髓性白血病（AML）中的錶達及臨床意義。方法採用CD45/SSC雙參數設門流式細胞術檢測120例A M L患者幼稚細胞錶麵及胞漿抗原錶達。結果在A M L中細胞膜抗原CD13、CD33和CD117錶達最常見，分彆為95．0％、95．0％和79．2％， CD117特異性高于CD13和CD33（ P ＜0．01）；胞漿抗原cM PO敏感性和特異性與CD117、胞漿抗原CD19敏感性與cCD79a比較差異均無統計學意義（ P ＞20．05），但cCD79a的特異性高于CD19（ P ＜0．01）。結論多參數流式細胞數檢測細胞繫列特異性胞漿抗原錶達是診斷急性髓性白血病的一項可靠技術。
목적：탐토세포계렬특이성항원재급성수성백혈병（AML）중적표체급림상의의。방법채용CD45/SSC쌍삼수설문류식세포술검측120례A M L환자유치세포표면급포장항원표체。결과재A M L중세포막항원CD13、CD33화CD117표체최상견，분별위95．0％、95．0％화79．2％， CD117특이성고우CD13화CD33（ P ＜0．01）；포장항원cM PO민감성화특이성여CD117、포장항원CD19민감성여cCD79a비교차이균무통계학의의（ P ＞20．05），단cCD79a적특이성고우CD19（ P ＜0．01）。결론다삼수류식세포수검측세포계렬특이성포장항원표체시진단급성수성백혈병적일항가고기술。
Objective To explore the expressions and clinical significance of serial specific antigens in pa-tients with acute myeloid leukemia(AML) .Methods Two-parameter of CD45/SSC gating flow cytometry was used to analyze expressions of the surface and cytoplasmic antigen in juvenile cells of 120 AML pa-tients .Results The expressions of CD13 (95 .0% ) ,CD33 (95 .0% ) and CD117 (79 .2% ) were the common in AML patients ,and the specificity of CD117 was higher than that of CD13 and CD33( P <0 .01) .There were no significant differences in sensitivity and specificity between cytoplasmic antigen cMPO and surface antigen CD117( P >0 .05) .No statistical significance in sensitivity was found between cytoplasmic anti-gens cCD79a and CD19 ( P >0 .05) ,but the specificity of cCD79 was higher than that of CD19( P <0 . 01) .Conclusion Muli-parameter flow cytometry is a reliable technique in diagnosing AML .
