CAJ | 학술논문

本文从分泌代谢物种类、最佳碳源、氮源、无机盐、微量元素、pH 和温度方面研究了前期筛选获得的对水稻细菌性条斑病有良好防效的解淀粉芽胞杆菌Bacillus amyloliquefaciens Lx-11的生物学特性。试验结果表明，解淀粉芽胞杆菌Lx-11可产生蛋白酶、纤维素酶和嗜铁素；最适生长温度为28℃、最适pH为7.0、最佳碳源为蔗糖，最佳氮源为胰蛋白胨，微量元素甘氨酸（Gly）、谷氨酸（Glu）、VB2、VB6和肌醇均能促进Lx-11的生长，其中Gly促进作用明显；无机盐KH2PO3和NaCl能促进Lx-11的生长；生防菌Lx-11在32 h前处于对数生长期，32 h之后，菌体生长基本达到稳定。正交试验所得最优培养基组合为酵母膏1 g/L、蔗糖5 g/L、胰蛋白胨6 g/L、甘氨酸0.01 g/L和氯化钠0.15 g/L。
본문종분비대사물충류、최가탄원、담원、무궤염、미량원소、pH 화온도방면연구료전기사선획득적대수도세균성조반병유량호방효적해정분아포간균Bacillus amyloliquefaciens Lx-11적생물학특성。시험결과표명，해정분아포간균Lx-11가산생단백매、섬유소매화기철소；최괄생장온도위28℃、최괄pH위7.0、최가탄원위자당，최가담원위이단백동，미량원소감안산（Gly）、곡안산（Glu）、VB2、VB6화기순균능촉진Lx-11적생장，기중Gly촉진작용명현；무궤염KH2PO3화NaCl능촉진Lx-11적생장；생방균Lx-11재32 h전처우대수생장기，32 h지후，균체생장기본체도은정。정교시험소득최우배양기조합위효모고1 g/L、자당5 g/L、이단백동6 g/L、감안산0.01 g/L화록화납0.15 g/L。
In order to understand the mode of action ofBacillus amyloliquefaciens Lx-11, we studied its biological characteristics in respects of temperature, pH, carbon/nitrogen sources, trace elements, inorganic salt and growth curve. Results showed that 28℃, pH 7.0, sucrose, soybean powder, Gly, KH2PO3and NaCl were optimum for its growth. The optimal culture medium compositions of the orthogonal experiment were yeast extract 1 g/L, sucrose 5 g/L, tryptone 6 g/L, glycine 0.01 g/L and sodium chloride 0.15 g/L. Logarithmic growth was at the first 32 h.