CAJ | 학술논문

从青岛黄海海域采集的海泥中分离得到了1株具有较高拮抗活性的菌株QD-10，通过培养性状、形态特征观察、生理生化特性测定及16S rDNA、ITS、gyrA和rpoB基因部分序列比对，将其鉴定为解淀粉芽胞杆菌Bacillus amyloliquefaciens。该菌株对本研究中选择的14种常见植物病原真菌和平菇褐斑病菌Pseudomonas tolaasii、大白菜黑腐病菌Xanthomonas campestrispv.campsetris 2种植物病原细菌均有不同程度的抑制作用，具有较宽的抑菌谱。该菌株能够产生嗜铁素、蛋白酶、纤维素酶和吲哚乙酸（IAA），表明其具备一定的生防相关性状。该菌株的不同浓度发酵液对番茄灰霉病的防效测定结果表明，在人工接种后第7 d和第11 d，菌株QD-10的发酵原液处理的防效均为100%，稀释5倍的发酵液防效分别为100%和41.7%，且二者均显著高于多抗霉素和多菌灵的防效。
종청도황해해역채집적해니중분리득도료1주구유교고길항활성적균주QD-10，통과배양성상、형태특정관찰、생리생화특성측정급16S rDNA、ITS、gyrA화rpoB기인부분서렬비대，장기감정위해정분아포간균Bacillus amyloliquefaciens。해균주대본연구중선택적14충상견식물병원진균화평고갈반병균Pseudomonas tolaasii、대백채흑부병균Xanthomonas campestrispv.campsetris 2충식물병원세균균유불동정도적억제작용，구유교관적억균보。해균주능구산생기철소、단백매、섬유소매화신타을산（IAA），표명기구비일정적생방상관성상。해균주적불동농도발효액대번가회매병적방효측정결과표명，재인공접충후제7 d화제11 d，균주QD-10적발효원액처리적방효균위100%，희석5배적발효액방효분별위100%화41.7%，차이자균현저고우다항매소화다균령적방효。
Strain QD-10 isolated from mud in Yellow Sea near Qingdao, Shandong Province, showed highly antagonistic activities in inhibiting fungi and bacteria.It was identified asBacillus amyloliquefaciens based on morphological, physiological and biochemical characteristics. The strain had a broad antimicrobial spectrum according to the tests on 14 plant pathogenic fungi, and 2 pathogenic bacteria. The strain was capable of producing siderophore, protease, cellulase and IAA.Treatment of tomato fruits with the fermentation broth of the strain at original and 5 times diluted solution could reduce gray mold by 100% and 41.7% at the 11th day after inoculation, respectively, higher than that of treatments with polyoxin and carbendazim.