CAJ | 학술논문

为了明确重寄生真菌盾壳霉Coniothyrium minitans产生的抗真菌物质（antifungal substances，简称AFS）的基本特性，本文从盾壳霉MCD培养物滤液的抑菌谱、热稳定性、酸碱稳定性及紫外线照射稳定性4个方面展开研究。研究结果表明，盾壳霉 AFS对核盘菌 Sclerotinia sclerotiorum、三叶草核盘菌S. trifoliorum、小核盘菌S. minior等10种真菌菌丝生长具有较强的抑制作用。AFS具有较强的热稳定性，盾壳霉MCD培养物滤液经120℃处理30 min后，对核盘菌仍具有抑菌活性（抑菌率为49.5%）。不同环境pH对AFS的抑菌活性具有不同的影响，pH值为7，AFS对核盘菌菌丝生长抑制活性最强。此外，环境pH也影响AFS的稳定性，在较宽的pH值范围内（1～14），尤其是在酸性条件下（pH 1～6），AFS活性稳定（抑菌率为81.1%～76.4%）；AFS对紫外线照射具有一定的稳定性。盾壳霉抗真菌物质对外界环境具有很好的稳定性，这对于应用盾壳霉的抗真菌物质来防治油菜菌核病是非常有利的。
위료명학중기생진균순각매Coniothyrium minitans산생적항진균물질（antifungal substances，간칭AFS）적기본특성，본문종순각매MCD배양물려액적억균보、열은정성、산감은정성급자외선조사은정성4개방면전개연구。연구결과표명，순각매 AFS대핵반균 Sclerotinia sclerotiorum、삼협초핵반균S. trifoliorum、소핵반균S. minior등10충진균균사생장구유교강적억제작용。AFS구유교강적열은정성，순각매MCD배양물려액경120℃처리30 min후，대핵반균잉구유억균활성（억균솔위49.5%）。불동배경pH대AFS적억균활성구유불동적영향，pH치위7，AFS대핵반균균사생장억제활성최강。차외，배경pH야영향AFS적은정성，재교관적pH치범위내（1～14），우기시재산성조건하（pH 1～6），AFS활성은정（억균솔위81.1%～76.4%）；AFS대자외선조사구유일정적은정성。순각매항진균물질대외계배경구유흔호적은정성，저대우응용순각매적항진균물질래방치유채균핵병시비상유리적。
The present study investigated the inhibitory range of antifungal substances (AFS) produced by Coniothyrium minitans against different phytopathogenic fungi and its stability under heat, acidic or alkaline conditions, and ultraviolet (UV). The results showed that the AFS could greatly inhibit the mycelia growth of the 10 tested fungi, includingSclerotinia sclerotiorum,S. trifoliorum andS. minior, and was heat-stable. The AFS heated at 120℃ for 30 min could inhibit mycelia growth ofS. sclerotiorum by 49.5%. pH could affect the activity of AFS againstS. sclerotiorum. The highest activity of AFS was observed under pH 7. The AFS was generally stable under different pH values (pH 1 to 14), but was more stable under acidic conditions (pH 1 to 6), with the inhibition of mycelia growth ranging from 81.1% to 76.4%. The stability of AFS could be affected by UV-irradiation when it was spread on leaves of oilseed rape. Based on these results, the AFS was concluded as stable substances, and considered to be of potential for control of rapeseed stem rot caused byS. sclerotiorum.