CAJ | 학술논문

菌株CF05是从浙江天目山柳杉中分离得到的1株细菌，经形态、16S rDNA序列、生理生化指标检测，鉴定为多粘类芽孢杆菌Paenibacillus polymyxa。平板拮抗结果表明，该菌对9种植物病原真菌和3种病原细菌具有显著拮抗效果。室内生测结果表明，发酵液和菌悬液对番茄幼苗均具有明显的促生效果，其中发酵液处理最佳，鲜重增长272.0%，干重增长266.7%。菌株CF05接种27 d后，番茄根际细菌浓度仍很高。种子萌发测定结果表明，菌株 CF05处理后发芽率提高11.7%，番茄猝倒病防治测定结果显示，发病率降低38.6%，效果均优于Bacillus subtilis 168处理。综上结果，表明P. polymyxa CF05是1株生防性状优良的促生细菌。
균주CF05시종절강천목산류삼중분리득도적1주세균，경형태、16S rDNA서렬、생리생화지표검측，감정위다점류아포간균Paenibacillus polymyxa。평판길항결과표명，해균대9충식물병원진균화3충병원세균구유현저길항효과。실내생측결과표명，발효액화균현액대번가유묘균구유명현적촉생효과，기중발효액처리최가，선중증장272.0%，간중증장266.7%。균주CF05접충27 d후，번가근제세균농도잉흔고。충자맹발측정결과표명，균주 CF05처리후발아솔제고11.7%，번가졸도병방치측정결과현시，발병솔강저38.6%，효과균우우Bacillus subtilis 168처리。종상결과，표명P. polymyxa CF05시1주생방성상우량적촉생세균。
Strain CF05 isolated from Cryptomeria fortunei in Tianmu Mountain, Zhejiang province was identified asPaenibacillus polymyxa on the bases of its phenotypical and biochemical characteristics as well as 16S rDNA gene sequence. TheP. polymyxaCF05 exhibited highly antagonistic effects against 9 plant fungal pathogens and 3 plant bacterial pathogensin vitro. Furthermore, both cell-free fermentation and bacterial suspension promoted tomato seedlings growth in greenhouse assay. Cell-free fermentation increased the biomass with fresh weight and dry weight by 272.0% and 266.7%, respectively. It was demonstrated that secondary metabolites from this bacterium might perform a critical function in plant growth promotion. Tests of root-colonizing ability indicated that concentrations of the bacterium in tomato rhizosphere were 3.8, 3.6, 3.1 and 3.1 log CFU/g root after 27 days of inoculation of tomato seeds with the bacterial suspension at 108、107、106、105 CFU/mL, respectively. Seed germination ratio was increased by 11.7%, and the disease incidence of damping-off was reduced by 38.6% after seed treatment.