CAJ | 학술논문

为研制安全、高效环保型保育猪中药添加剂，将72头体重相近的28日龄杜长大保育猪随机分为4组，每组3个重复，每个重复6头猪，对照组、抗生素组、中药Ⅰ组、中药Ⅱ组分别饲喂基础饲粮、基础饲粮+抗生素、基础饲粮+1%中草药组方Ⅰ、基础饲粮+1%中草药组方Ⅱ，进行为期28 d的饲养试验，测定保育猪肠道pH、微生物区系及小肠黏膜绒毛高度与隐窝深度。结果显示，中药Ⅱ组效果优于中药Ⅰ组。中药Ⅱ组与对照组相比，可显著降低空肠、回肠pH（P＜0.05），对盲肠pH影响不大（P＞0.05）；可极显著降低空肠、回肠、盲肠的大肠杆菌浓度（P＜0.01）；显著提高空肠、回肠、盲肠的乳酸杆菌浓度（P＜0.05）；可显著提高空肠、回肠的双歧杆菌浓度（P＜0.05），对盲肠的双歧杆菌浓度影响不大（P＞0.05）；可显著提高十二指肠的绒毛高度（P＜0.05），极显著提高空肠、回肠的绒毛高度（P＜0.01）；对隐窝深度影响不大（P＞0.05）；极显著提高绒毛高度与隐窝深度的比值（P＜0.01）。结果表明，中药Ⅱ组能刺激保育猪肠道乳酸杆菌和双歧杆菌的增殖、抑制大肠杆菌的增殖并有效改善小肠黏膜形态。
위연제안전、고효배보형보육저중약첨가제，장72두체중상근적28일령두장대보육저수궤분위4조，매조3개중복，매개중복6두저，대조조、항생소조、중약Ⅰ조、중약Ⅱ조분별사위기출사량、기출사량+항생소、기출사량+1%중초약조방Ⅰ、기출사량+1%중초약조방Ⅱ，진행위기28 d적사양시험，측정보육저장도pH、미생물구계급소장점막융모고도여은와심도。결과현시，중약Ⅱ조효과우우중약Ⅰ조。중약Ⅱ조여대조조상비，가현저강저공장、회장pH（P＜0.05），대맹장pH영향불대（P＞0.05）；가겁현저강저공장、회장、맹장적대장간균농도（P＜0.01）；현저제고공장、회장、맹장적유산간균농도（P＜0.05）；가현저제고공장、회장적쌍기간균농도（P＜0.05），대맹장적쌍기간균농도영향불대（P＞0.05）；가현저제고십이지장적융모고도（P＜0.05），겁현저제고공장、회장적융모고도（P＜0.01）；대은와심도영향불대（P＞0.05）；겁현저제고융모고도여은와심도적비치（P＜0.01）。결과표명，중약Ⅱ조능자격보육저장도유산간균화쌍기간균적증식、억제대장간균적증식병유효개선소장점막형태。
For the development of a safe, efficient and environmentally friendly Chinese herb additive in weaned piglets, 72 Duroc-Landrace-Yorkshire weaned piglets which were randomly divided into four groups, each treatment had 3 replicates and each consisting of 6 piglets. The control group was fed the basal diet. Antibiotic group was fed the basal diet plus antibiotics. Chinese herbsⅠgroup was added 1% Chinese herbs formulaⅠ, Chinese herbsⅡgroup was added 1% Chinese herbs formulaⅡ. Feeding trial lasted 28 days, and the intestinal pH, microflora, intestinal mucosal villus height and crypt depth were measured. The results were as followed:Chinese herbsⅡgroup are better than Chinese herbsⅠgroup. Compared with the control group, Chinese herbsⅡgroup can significantly reduce the pH value in the jejunum and ileum (P<0.05), but little effect on the pH value of the cecum (P>0.05). Compared with the control group, Chinese herbsⅡgroup can significantly reduce the E. coli concentration (P<0.01) and improve the lactobacilli concentration (P<0.05) in the jejunum, ileum and cecum, Chinese herbsⅡgroup can also significantly increase the bifidobacteria concentration in the jejunum and ileum (P<0.05), but little effect on the bifidobacteria concentration in the cecum (P>0.05). Compared with the control group, Chinese herbsⅡgroup can significantly improve the duodenal villus height (P<0.05) and significantly increase the villus height in the jejunum and ileum (P<0.01). Compared with the control group, Chinese herbsⅡgroup can significantly increase the ratio of villus height and crypt depth (P<0.01), but little effect on the crypt depth (P>0.05). These indicate that Chinese herbsⅡgroup can stimulate the intestinal lactobacilli and bifidobacteria proliferation, inhibit the proliferation of E. coli and effectively improve the intestinal mucosal morphology.