CAJ | 학술논문

采用硅胶柱色谱、重结晶、高效液相色谱半制备法等方法,对羊脆木(Pittosporum kerrii Craib)树皮的氯仿、甲醇部位进行化学成分研究,分离纯化得7个单体,通过波谱分析鉴定其结构,分别为：β-stigmsterol(1)、β-stigmasterol-3-O-β-D-glucopyranoside(2)、ethyl caffeate(3)、2,6-dimethoxy-1,4-benzoquinone(4)、3-hydroxy-4-methoxybenzoic acid(5)、syringaresinol-4,4′-di-O-β-D-glucoside (6)、3,4,5-trimethoxyphenol-1-O-β-D-glucopyranoside(7),其中4、5为首次从该属中分离得到.细胞毒试验表明氯仿提取物及从其中分离出来的化合物3、4都具有明显的细胞毒活性,对K562细胞的IC50分别为3.7、4.0、3.0μg/mL.
채용규효주색보、중결정、고효액상색보반제비법등방법,대양취목(Pittosporum kerrii Craib)수피적록방、갑순부위진행화학성분연구,분리순화득7개단체,통과파보분석감정기결구,분별위：β-stigmsterol(1)、β-stigmasterol-3-O-β-D-glucopyranoside(2)、ethyl caffeate(3)、2,6-dimethoxy-1,4-benzoquinone(4)、3-hydroxy-4-methoxybenzoic acid(5)、syringaresinol-4,4′-di-O-β-D-glucoside (6)、3,4,5-trimethoxyphenol-1-O-β-D-glucopyranoside(7),기중4、5위수차종해속중분리득도.세포독시험표명록방제취물급종기중분리출래적화합물3、4도구유명현적세포독활성,대K562세포적IC50분별위3.7、4.0、3.0μg/mL.
The constituents in the chloroform and methanol extract of the bark of Pittosporum kerrii Craib were isolated by silicagel column chromatography,recrystallization,semipreparative HPLC and so on.Their structures were identified by spectral and chemical analysis.Seven com-pounds were isolated and identified asβ-stigmsterol (1 ),β-stigmasterol-3-O-β-D-glucopyranoside (2),ethyl caffeate(3),2,6-dimethoxy-1,4-benzoquinone(4),3-hydroxyl-4-methoxybenzoic acid(5), syringeresinol-4,4′-di-O-β-D-glucoside (6 )and 3,4,5-trimethoxyphenol-1-O-β-D-glucopyranoside (7).Compound 4,5 are obtained and reported from Pittosporum for the first time.The chloroform extract and compounds 3,4 in which it is separated from chloroform extract possess obvious ac-tivities showed by cytotoxic activities tests,the IC50 are 3.7,4.0,3.0μg/mL for K562 cell,respec-tively.