CAJ | 학술논문

目的：评价昆明种小鼠哪一鼠龄段为甲型流感病毒FM1株最佳模型。方法150只清洁级昆明种小鼠，雌雄各半，鼠龄均等，分为幼龄模型组（40只）、成龄模型组（40只）、老龄模型组（40只）、幼龄正常组（10只）、成龄正常组（10只）、老龄正常组（10只），观察小鼠状态，分别于感染3、5、7 d，检测其肺湿质量、肺组织肉眼病理评分、肺组织病理切片以及肺组织FQ-PCR病毒载量。结果幼龄模型组14 d内死亡最多，整体状态最差，肺部病变最为严重，肺组织病毒载量呈递增趋势。成龄模型组14 d内死亡最少，整体状态最好，肺部病变少，肺组织病毒载量无复制的现象。老龄模型组整体状态在第3天出现肺组织病变，但之后有恢复的趋势，第5天后，病毒载量呈下降趋势。结论幼龄昆明种小鼠为甲型流感病毒最佳理想模型。
목적：평개곤명충소서나일서령단위갑형류감병독FM1주최가모형。방법150지청길급곤명충소서，자웅각반，서령균등，분위유령모형조（40지）、성령모형조（40지）、노령모형조（40지）、유령정상조（10지）、성령정상조（10지）、노령정상조（10지），관찰소서상태，분별우감염3、5、7 d，검측기폐습질량、폐조직육안병리평분、폐조직병리절편이급폐조직FQ-PCR병독재량。결과유령모형조14 d내사망최다，정체상태최차，폐부병변최위엄중，폐조직병독재량정체증추세。성령모형조14 d내사망최소，정체상태최호，폐부병변소，폐조직병독재량무복제적현상。노령모형조정체상태재제3천출현폐조직병변，단지후유회복적추세，제5천후，병독재량정하강추세。결론유령곤명충소서위갑형류감병독최가이상모형。
Objective To evaluate which rat age segment of Kunming mice is the best model of influ-enza A virus FM1 strains.Methods A total 150 Kunming mice of clean grade,half male and half female, with equal age,were randomly divided into young model group (40 rats),mature model group (40 rats), aged model group (40 rats),young normal group (10 rats),mature normal group (10 rats),and aged nor-mal group (10 rats).The mice were observed,and respectively at the end of 3,5 and 7 days of infection were detected lung wet weight;lung tissue pathology score with naked eyes,lung histopathology and lung tissue FQ-PCR viral load testing were also performed.Results In the young model group,the highest death occurred in 14 days,the overall state was the worst with most severe lung disease,and viral load in lung tis-sue showed an increasing trend.In mature model group,the death was the least in 14 days,the overall state was the best,basically there was no lung disease,and lung tissue viral load had no copying phenomenon.In aged rats,the overall state showed lung tissue lesions in the first three days,but there was a tendency to recover and af-ter 5 days,viral load tended to decline.Conclusion The young mice are the idealest model of influenza A virus.