CAJ | 학술논문

动物源医疗器械的免疫原性是评价其免疫毒理学安全性的关键和难点。该文利用MTT法和CFSE法体外研究动物源性胶原蛋白海绵浸提液对小鼠脾脏淋巴细胞转化和增殖作用，并对两种方法的影响因素进行了分析。研究表明，胶原蛋白海绵浸提液未引起小鼠体外脾脏淋巴细胞发生明显转化和增殖。
동물원의료기계적면역원성시평개기면역독이학안전성적관건화난점。해문이용MTT법화CFSE법체외연구동물원성효원단백해면침제액대소서비장림파세포전화화증식작용，병대량충방법적영향인소진행료분석。연구표명，효원단백해면침제액미인기소서체외비장림파세포발생명현전화화증식。
Immunogenicity for medical devices of animal origin is the key and diffi cult point during immune safety evaluation for these devices. This paper firstly investigated the effect of colagen sponge of animal origin on mouse splenic lymphocyte transformation and proliferation, and then analyzed the infl uence factors on the MTT method and CFSE method. The results showed that colagen sponge extract cannot signifi cantly induce transformation and proliferation of mouse splenic lymphocyte in vitro.