西部林业科学
西部林業科學
서부임업과학
JOURNAL OF WEST CHINA FORESTRY SCIENCE
2014年
4期
44-48
,共5页
曾嵘%李祖红%赵银朵%赵明富%国松%田应涛%张芬芬%张拯研%李永忠
曾嶸%李祖紅%趙銀朵%趙明富%國鬆%田應濤%張芬芬%張拯研%李永忠
증영%리조홍%조은타%조명부%국송%전응도%장분분%장증연%리영충
烟草黑胫病%微生物菌剂%抗性酶%丙二醛
煙草黑脛病%微生物菌劑%抗性酶%丙二醛
연초흑경병%미생물균제%항성매%병이철
tobacco black shank%bio-controlling strain%resistant enzyme%malondialdehyde
为了探究生防菌对烟草抗病性的影响，2012年，以对黑胫病抗性不同的高抗品种（云烟87）、中抗品种（ K326）和感病品种（红花大金元）等3种烟草品种70天苗龄的实生苗为实验材料，在烟苗茎秆接种黑胫病原后施用该生防菌，于不同接种时期（0天、15天、30天、45天、60天、75天）测定叶片中苯丙氨酸解氨酶（PAL）、多酚氧化酶（PPO）、过氧化物酶（POD）的活性及丙二醛（MDA）含量。结果表明，（1）高抗品种、中抗品种和感病品种的PAL、 PPO及POD活性水平皆明显高于各自的对照，且前期增加速度表现为高抗品种＞中抗品种＞感病品种，后期PPO活性减小情况也符合以上规律。（2）高抗品种、中抗品种和感病品种的MDA的含量增加速度明显低于各自的对照，前期MDA含量增加的速度和后期降低的速度均是感病品种＞中抗品种＞高抗品种。可见，施用该生防菌可通过调节抗性酶活性和MDA含量提高烟株对烟草黑胫病的抗病性。
為瞭探究生防菌對煙草抗病性的影響，2012年，以對黑脛病抗性不同的高抗品種（雲煙87）、中抗品種（ K326）和感病品種（紅花大金元）等3種煙草品種70天苗齡的實生苗為實驗材料，在煙苗莖稈接種黑脛病原後施用該生防菌，于不同接種時期（0天、15天、30天、45天、60天、75天）測定葉片中苯丙氨痠解氨酶（PAL）、多酚氧化酶（PPO）、過氧化物酶（POD）的活性及丙二醛（MDA）含量。結果錶明，（1）高抗品種、中抗品種和感病品種的PAL、 PPO及POD活性水平皆明顯高于各自的對照，且前期增加速度錶現為高抗品種＞中抗品種＞感病品種，後期PPO活性減小情況也符閤以上規律。（2）高抗品種、中抗品種和感病品種的MDA的含量增加速度明顯低于各自的對照，前期MDA含量增加的速度和後期降低的速度均是感病品種＞中抗品種＞高抗品種。可見，施用該生防菌可通過調節抗性酶活性和MDA含量提高煙株對煙草黑脛病的抗病性。
위료탐구생방균대연초항병성적영향，2012년，이대흑경병항성불동적고항품충（운연87）、중항품충（ K326）화감병품충（홍화대금원）등3충연초품충70천묘령적실생묘위실험재료，재연묘경간접충흑경병원후시용해생방균，우불동접충시기（0천、15천、30천、45천、60천、75천）측정협편중분병안산해안매（PAL）、다분양화매（PPO）、과양화물매（POD）적활성급병이철（MDA）함량。결과표명，（1）고항품충、중항품충화감병품충적PAL、 PPO급POD활성수평개명현고우각자적대조，차전기증가속도표현위고항품충＞중항품충＞감병품충，후기PPO활성감소정황야부합이상규률。（2）고항품충、중항품충화감병품충적MDA적함량증가속도명현저우각자적대조，전기MDA함량증가적속도화후기강저적속도균시감병품충＞중항품충＞고항품충。가견，시용해생방균가통과조절항성매활성화MDA함량제고연주대연초흑경병적항병성。
For understanding the effect of a bio-bacterial strain on tobacco disease resistance , three 70-day-tobac-co cultivars with different resistance to black shank were used as materials in 2012.Phenylalanine ammonia lyase ( PAL) , polyphenol oxidase ( PPO) , peroxidase ( POD) and content of malondialdehyde ( MDA) in different pe-riod (0 d, 15 d, 30 d, 45 d, 60 d and 75 d) after infected with pathogen of tobacco black shank and applica-tion of bio-bacterial strain The results showed that: 1) PAL, PPO and POD of high resistant variety , resistant va-riety and susceptible variety were much higher than those of their control , and the increasing speed at early stage showed as high resistant variety >resistant variety>susceptible variety , and decreased of PPO activity later was al-so comply with the above rules .2) The increase speed of MDA content of high resistant variety , resistant variety and susceptible variety was much lower than those of their control , and the increasing speed at early stage and de-creasing speed at later stage of MDA content showed as susceptible variety >resistant variety >high resistant varie-ty.In this sense , by adusting resistant enzyme activity and MDA content , tobacco black shank disease resistance could be improved .
