生物学杂志
生物學雜誌
생물학잡지
JOURNAL OF BIOLOGY
2014年
4期
55-59
,共5页
俞国伟%魏春%张艳丽%汪钊
俞國偉%魏春%張豔麗%汪釗
유국위%위춘%장염려%왕쇠
胶红酵母%番茄渣%发酵%类胡萝卜素
膠紅酵母%番茄渣%髮酵%類鬍蘿蔔素
효홍효모%번가사%발효%류호라복소
Rhodotorula mucilaginosa%tomato pomace%fermentation%carotenoid
基于降低微生物类胡萝卜素生产成本的考虑,采用番茄渣、豆粕的纤维素酶酶解产物培养胶红酵母,以单位体积发酵液中的总类胡萝卜素浓度增量作为优化目标,先后运用逐步单因素法和均匀设计法系统性地考查了胶红酵母的总类胡萝卜素产量和增量与各个相关因素之间的关系。实验获得的总类胡萝卜素最大产量以及扣除了番茄渣中的类胡萝卜素含量而计算得到的增量分别为12.25 mg/L和10.25 mg/L。实验结果证明设计的生产工艺能够以较低的成本生产出富含类胡萝卜素的饲料,因而是经济可行的。
基于降低微生物類鬍蘿蔔素生產成本的攷慮,採用番茄渣、豆粕的纖維素酶酶解產物培養膠紅酵母,以單位體積髮酵液中的總類鬍蘿蔔素濃度增量作為優化目標,先後運用逐步單因素法和均勻設計法繫統性地攷查瞭膠紅酵母的總類鬍蘿蔔素產量和增量與各箇相關因素之間的關繫。實驗穫得的總類鬍蘿蔔素最大產量以及釦除瞭番茄渣中的類鬍蘿蔔素含量而計算得到的增量分彆為12.25 mg/L和10.25 mg/L。實驗結果證明設計的生產工藝能夠以較低的成本生產齣富含類鬍蘿蔔素的飼料,因而是經濟可行的。
기우강저미생물류호라복소생산성본적고필,채용번가사、두박적섬유소매매해산물배양효홍효모,이단위체적발효액중적총류호라복소농도증량작위우화목표,선후운용축보단인소법화균균설계법계통성지고사료효홍효모적총류호라복소산량화증량여각개상관인소지간적관계。실험획득적총류호라복소최대산량이급구제료번가사중적류호라복소함량이계산득도적증량분별위12.25 mg/L화10.25 mg/L。실험결과증명설계적생산공예능구이교저적성본생산출부함류호라복소적사료,인이시경제가행적。
In this study, the enzymatically hydrolyzed product of tomato pomace and soybean meal were used for culturing Rhodotorula mucilaginosa, based on the consideration of decreasing the cost of production of microbial carotenoids .The increment of total carote-noids concentration in unit volume was chosen as the optimized target .By sequentially employing stepwise single-factor experiments and uniform design , the relationship between total carotenoids production of Rhodotorula mucilaginosa and each of the relevant factors was systematically investigated .The experimentally obtained total carotenoids yield and increment calculated through deducting the total ca -rotenoids content in tomato pomace were 12.25 mg/L and 10.25 mg/L, respectively.The experimental results proved that the produc-tion process designed in this study was able to produce carotenoid-enriched feedstuff with lower cost of production , and thus was eco-nomically feasible .