CAJ | 학술논문

以小鼠的肝、肺组织的正常细胞与肝肿瘤细胞（Hepa1-6）、肺肿瘤细胞（LLC）为研究对象，通过CTFM（cell traction force microscopy ）法测定了4组细胞系的牵引力；用荧光抗体染色技术比较了小鼠细胞的α-SMA蛋白在癌变前后的变化。实验发现：与小鼠正常细胞相比，在α-SMA的表达水平上，Hepa1-6细胞α-SMA的平均光密度减小了47.9％，LLC细胞下降了52.3％；而Hepa1-6细胞牵引力的均方根值减小了53.4％，LLC细胞减小了49.7％。这说明α-SMA的表达与牵引力的变化以及细胞癌变的过程是密切相关的。
이소서적간、폐조직적정상세포여간종류세포（Hepa1-6）、폐종류세포（LLC）위연구대상，통과CTFM（cell traction force microscopy ）법측정료4조세포계적견인력；용형광항체염색기술비교료소서세포적α-SMA단백재암변전후적변화。실험발현：여소서정상세포상비，재α-SMA적표체수평상，Hepa1-6세포α-SMA적평균광밀도감소료47.9％，LLC세포하강료52.3％；이Hepa1-6세포견인력적균방근치감소료53.4％，LLC세포감소료49.7％。저설명α-SMA적표체여견인력적변화이급세포암변적과정시밀절상관적。
The traction forces of hepatic cells , hepatoma cells (Hepa1-6) and lung cells, LLC cells of mouse were determined with CTFM( cell traction force microscopy ) method.The level of α-SMA expression before and after the canceration with the fluorescent an-tibody staining technique was compared .The results showed that the mean density of α-SMA expression level reduced by 47.9% in Hepa1-6 cells and 52.3%in LLC cells compared to that of hepatoma cells and lung cells of mouse .The RMS ( root mean square ) of the traction force of the Hepa 1-6 and LLC cells decreased by 53.4%and 49.7%, respectively .