CAJ | 학술논문

研究了纳米ZnO对斑马鱼肝脏生理生化指标的影响。当斑马鱼暴露在ZnO纳米颗粒浓度为2.8 mg/L、5.6 mg/L、11.2 mg/L、22.4 mg/L和44.8 mg/L时，分别测定了6 h、12 h、24 h、48 h和72 h时斑马鱼肝组织中的GSH、MDA、Na＋K＋-ATPase的含量和24 h ROS的变化，结果显示：与对照组相比，处理组中斑马鱼肝组织中GSH的含量显著减少（ P＜0.05），MDA含量随处理浓度的升高却显著增加（ P＜0.05），Na＋K＋-ATPase活性随暴露时间先显著降低后显著升高（ P＜0.05），24 h ROS含量高于对照组（ P＜0.05），说明斑马鱼肝组织的生理活动受到ZnO纳米颗粒的影响，且有时间和浓度依赖性。
연구료납미ZnO대반마어간장생리생화지표적영향。당반마어폭로재ZnO납미과립농도위2.8 mg/L、5.6 mg/L、11.2 mg/L、22.4 mg/L화44.8 mg/L시，분별측정료6 h、12 h、24 h、48 h화72 h시반마어간조직중적GSH、MDA、Na＋K＋-ATPase적함량화24 h ROS적변화，결과현시：여대조조상비，처리조중반마어간조직중GSH적함량현저감소（ P＜0.05），MDA함량수처리농도적승고각현저증가（ P＜0.05），Na＋K＋-ATPase활성수폭로시간선현저강저후현저승고（ P＜0.05），24 h ROS함량고우대조조（ P＜0.05），설명반마어간조직적생리활동수도ZnO납미과립적영향，차유시간화농도의뢰성。
The effects of nano ZnO on the physiological and biochemical index in the liver of zebra fish were studied in this paper .Ze-bra fish were exposed to the different concentration ( 8 mg/L, 5.6 mg/L, 11.2 mg/L, 22.4 mg/L and 44.8 mg/L) of the nano ZnO. The contents of GSH, MDA, and Na +K+-ATPase in the liver were detected at 6, 12, 24, 48, and 72 hours.Changes of 24 h ROS were also detected .The results showed that the content of GSH in the liver tissues was significantly lower than that in the control group ( p<0.05 ) , while the content of MDA in the liver tissues was significantly increased ( p<0.05 ) with the increasing concentration of nano ZnO.The activity of Na +/K+-ATP enzyme in liver tissues was significantly decreased at first , and then increased ( p<0.05 ) with a longer exposure time .The content of changes of 24 h ROS in the liver was higher than that in the control group ( p<0.05 ) .Ex-perimental results showed that physiology in the liver tissues of zebra fish was affected by the ZnO nanoparticles and also depended on the time and concentration .