CAJ | 학술논문

目的观察PKC-β抑制剂LY333531（LY）对糖尿病大鼠心肌功能及Caveolins蛋白的影响。方法24只SD雄性大鼠随机分为正常对照组（ C）、糖尿病组（ D）及糖尿病LY治疗组。4周后生化检测血浆甘油三酯（ TG）、炎症因子TNF-α与IL-6水平，心脏超声评价心脏功能，Western Blot分析心肌组织Cav-1、Cav-3，PKC-β2蛋白表达水平。结果与C组比较，D组大鼠血浆TG、TNF-α与IL-6水平均增高，心脏舒张功能降低，心肌组织p-PKC-β2、Cav-1表达水平增加而Cav-3表达水平降低。与D组比较，LY除了不能降低糖尿病大鼠血浆TNF-α与IL-6水平外，均可明显抑制上述变化。结论 PKC-β抑制剂LY明显改善糖尿病心肌舒张功能，其机制可能与抑制PKC-β2过度活化并恢复Caveolins蛋白表达水平有关。
목적관찰PKC-β억제제LY333531（LY）대당뇨병대서심기공능급Caveolins단백적영향。방법24지SD웅성대서수궤분위정상대조조（ C）、당뇨병조（ D）급당뇨병LY치료조。4주후생화검측혈장감유삼지（ TG）、염증인자TNF-α여IL-6수평，심장초성평개심장공능，Western Blot분석심기조직Cav-1、Cav-3，PKC-β2단백표체수평。결과여C조비교，D조대서혈장TG、TNF-α여IL-6수평균증고，심장서장공능강저，심기조직p-PKC-β2、Cav-1표체수평증가이Cav-3표체수평강저。여D조비교，LY제료불능강저당뇨병대서혈장TNF-α여IL-6수평외，균가명현억제상술변화。결론 PKC-β억제제LY명현개선당뇨병심기서장공능，기궤제가능여억제PKC-β2과도활화병회복Caveolins단백표체수평유관。
Objective To study the effect of LY333531(LY)on myocardiac function and expression of myocardial protein Caveolins in diabetic rats. Methods A total of 24 male SD rats were randomly divided into control group(C),diabetic group(D)and diabetic group with LY treatment for 4 weeks. The plasma levels of triglyceride(TG),inflammatory factor TNF - α and IL -6 were detected by biochemical analysis. The cardiac function was evaluated by echocardiography. The expression of Cav -1,Cav -3 and PKC - β2 were detected by Western blot. Results In comparison with group C,the plasma levels of TG,TNF - α and IL -6 in group D were significantly increased and accompanied with insulted cardiac diastolic function. The protein expression of PKC - β2 and Cav -1 in diabetic rats was up - regulated,but the protein expression of Cav - 3 was down - regulated as compared with that of control rats. The treatment with LY prevented or reversed all these changes without impacting plasma levels of TNF - α and IL -6. Conclusion Selective inhibition of PKC - β with LY can significantly improve diabetic cardiac diastolic function, it may be related to the inhibition of excessive activation of PKC - β2 and restore the expression of Caveolins.