中华行为医学与脑科学杂志
中華行為醫學與腦科學雜誌
중화행위의학여뇌과학잡지
CHINESE JOURNAL OF BEHAVIORAL MEDICINE AND BRAIN SCIENCE
2013年
6期
481-483
,共3页
精神分裂症%地卓西平马来酸盐%再认记忆
精神分裂癥%地卓西平馬來痠鹽%再認記憶
정신분렬증%지탁서평마래산염%재인기억
Schizophrenia%Dizocilpine maleate%Recognition memory
目的 观察成年期大鼠急性注射中低剂量地卓西平马来酸盐(MK-801)诱导的行为学变化,寻找塑造精神分裂症再认记忆缺陷模型适宜的药物剂量.方法 192只成年雄性大鼠以体质量按照分层随机化法分实验组和对照组,实验组给予不同剂量MK-801腹腔注射,对照组予生理盐水,独立进行旷场实验、前脉冲抑制(PPI)、新异物体识别实验.结果 1.给药后自发活动明显增加,表现出剂量依赖性的行为学效应,其中0.3 mg/kg组自发活动总距离[(127.04±32.35)m]在给药后显著高于对照组[(35.34±12.81)m,P＜0.05].2.MK-801呈剂量依赖性地抑制了PPI的水平(P＜0.01),0.1 mg/kg剂量组[(103.45±68.04)％]、0.3 mg/kg剂量组[(41.55±62.93)％]与盐水组[(200.39±30.97)％]比较差异有统计学意义(P＜0.05).3.0.03 mg/kg剂量组分辨指数[(15.78±6.23)％]、0.1 mg/kg剂量组分辨指数[(22.42±3.85)％]显著低于盐水组[(39.42±3.86)％,P＜0.05].结论 不同精神分裂症内表型需要选择恰当的MK-801剂量来模拟；0.03 mg/kg是模拟精神分裂症再认记忆缺陷模型相对适宜的剂量.
目的 觀察成年期大鼠急性註射中低劑量地卓西平馬來痠鹽(MK-801)誘導的行為學變化,尋找塑造精神分裂癥再認記憶缺陷模型適宜的藥物劑量.方法 192隻成年雄性大鼠以體質量按照分層隨機化法分實驗組和對照組,實驗組給予不同劑量MK-801腹腔註射,對照組予生理鹽水,獨立進行曠場實驗、前脈遲抑製(PPI)、新異物體識彆實驗.結果 1.給藥後自髮活動明顯增加,錶現齣劑量依賴性的行為學效應,其中0.3 mg/kg組自髮活動總距離[(127.04±32.35)m]在給藥後顯著高于對照組[(35.34±12.81)m,P＜0.05].2.MK-801呈劑量依賴性地抑製瞭PPI的水平(P＜0.01),0.1 mg/kg劑量組[(103.45±68.04)％]、0.3 mg/kg劑量組[(41.55±62.93)％]與鹽水組[(200.39±30.97)％]比較差異有統計學意義(P＜0.05).3.0.03 mg/kg劑量組分辨指數[(15.78±6.23)％]、0.1 mg/kg劑量組分辨指數[(22.42±3.85)％]顯著低于鹽水組[(39.42±3.86)％,P＜0.05].結論 不同精神分裂癥內錶型需要選擇恰噹的MK-801劑量來模擬；0.03 mg/kg是模擬精神分裂癥再認記憶缺陷模型相對適宜的劑量.
목적 관찰성년기대서급성주사중저제량지탁서평마래산염(MK-801)유도적행위학변화,심조소조정신분렬증재인기억결함모형괄의적약물제량.방법 192지성년웅성대서이체질량안조분층수궤화법분실험조화대조조,실험조급여불동제량MK-801복강주사,대조조여생리염수,독립진행광장실험、전맥충억제(PPI)、신이물체식별실험.결과 1.급약후자발활동명현증가,표현출제량의뢰성적행위학효응,기중0.3 mg/kg조자발활동총거리[(127.04±32.35)m]재급약후현저고우대조조[(35.34±12.81)m,P＜0.05].2.MK-801정제량의뢰성지억제료PPI적수평(P＜0.01),0.1 mg/kg제량조[(103.45±68.04)％]、0.3 mg/kg제량조[(41.55±62.93)％]여염수조[(200.39±30.97)％]비교차이유통계학의의(P＜0.05).3.0.03 mg/kg제량조분변지수[(15.78±6.23)％]、0.1 mg/kg제량조분변지수[(22.42±3.85)％]현저저우염수조[(39.42±3.86)％,P＜0.05].결론 불동정신분렬증내표형수요선택흡당적MK-801제량래모의；0.03 mg/kg시모의정신분렬증재인기억결함모형상대괄의적제량.
Objective To observe the behavioral effects of acute administration of dizocilpine (MK-801) in rats,and to determine an appropriate doses of the drug to mimicking memory deficits of schizophrenia.Methods 192 rats were randomly divided into model groups and control group,and given different doses of MK-801 or saline.Locomotor activity,pre-pulse inhibition (PPI) test and novel object recognition test were studied respectively.Results The spontaneous activity increased in a dose-dependent manner after the treatment of MK-801.The total distances of locomotor activity in 0.3 mg/kg group((127.04 ± 32.35) m) exhibited a significant difference compared with control group((35.34 ± 12.81) m,P ＜ 0.05).MK-801 decreased PPI in dose-dependent manner(P ＜ 0.01).MK-801 0.1 mg/kg((103.45 ± 68.04) ％) and 0.3 mg/kg ((41.55 ± 62.93) ％) groups showed significant differences compared to control group ((200.39 ± 30.97) ％) in PPI.The discrimination index of 0.03 mg/kg group((15.78 ± 6.23) ％) and 0.1 mg/kg group((22.42 ± 3.85) ％) were lower than that in control group((39.42 ±3.86)％,P＜0.05).Conclusion It is necessary for select right doses for model different endophenotypes of schizophrenia by MK-801.0.03 mg/kg of MK-801 is a relatively appropriate dosage to cause recognition memory damage.
