CAJ | 학술논문

目的观察骨髓间质干细胞植入后能否改善血管痉挛大鼠的感觉、运动及学习记忆功能.方法实验动物按随机数字表法分为对照组、蛛网膜下腔出血组、干细胞培养液组和干细胞移植组,蛛网膜下腔出血模型利用SD大鼠自体尾动脉取血注血造模,第2次注血后2d,传代后的大鼠间质干细胞侧脑室植入.蛛网膜下腔出血组(Subarachnoid hemorrhage,SAH组)不植入干细胞,培养液组仅注入干细胞培养液(Dulbecco's modified eagle medium,DMEM组),骨髓间质干细胞移植组(Bone Marrow Mesenchymal Stem Cells,BMSCs组)注入30μl干细胞悬液.利用水迷宫实验和动物神经功能评分测量各组动物的神经功能评分及学习记忆功能.结果干细胞植入后7d时对照组、SAH组、DMEM组和干细胞组神经功能评分为(3.95±2.51)分、(7.20±1.03)分、(7.23±1.79)分和(5.81 ±1.11)分,与前2组相比,干细胞组神经生物功能评分明显减低(P=0.017).干细胞移植后14 d时对照组、SAH组、DMEM组和干细胞组平均跨越平台次数为(7.38±1.73)次；(4.52±0.90)次；(5.11±1.93)次和(7.32±2.16)次,其中与SAH组和DMEM组相比,干细胞移植组和对照组跨越平台次数差异有统计学意义(P=0.009),而对照组和干细胞移植组相比,跨越平台次数差异无统计学意义(P=0.14).结论大鼠脑血管痉挛后间质干细胞植入对感觉、运动功能及学习记忆功能都有一定的改善.
목적 관찰골수간질간세포식입후능부개선혈관경련대서적감각、운동급학습기억공능.방법 실험동물안수궤수자표법분위대조조、주망막하강출혈조、간세포배양액조화간세포이식조,주망막하강출혈모형이용SD대서자체미동맥취혈주혈조모,제2차주혈후2d,전대후적대서간질간세포측뇌실식입.주망막하강출혈조(Subarachnoid hemorrhage,SAH조)불식입간세포,배양액조부주입간세포배양액(Dulbecco's modified eagle medium,DMEM조),골수간질간세포이식조(Bone Marrow Mesenchymal Stem Cells,BMSCs조)주입30μl간세포현액.이용수미궁실험화동물신경공능평분측량각조동물적신경공능평분급학습기억공능.결과 간세포식입후7d시대조조、SAH조、DMEM조화간세포조신경공능평분위(3.95±2.51)분、(7.20±1.03)분、(7.23±1.79)분화(5.81 ±1.11)분,여전2조상비,간세포조신경생물공능평분명현감저(P=0.017).간세포이식후14 d시대조조、SAH조、DMEM조화간세포조평균과월평태차수위(7.38±1.73)차；(4.52±0.90)차；(5.11±1.93)차화(7.32±2.16)차,기중여SAH조화DMEM조상비,간세포이식조화대조조과월평태차수차이유통계학의의(P=0.009),이대조조화간세포이식조상비,과월평태차수차이무통계학의의(P=0.14).결론 대서뇌혈관경련후간질간세포식입대감각、운동공능급학습기억공능도유일정적개선.
Objective To observe whether bone marrow mesenchymal stem cell transplantation can improve vasospastic rats sense and motor function.Methods Rats grouped with randomized number method as Control group,Subarachnoid hemorrhage group.Stem cell culture media group and Stem cell transplantation group.Subarachnoid hemorrhage model were made with tail artery blood twice injection,2 days after 2' nd injection.Bone marrow mesenchymal stem cell were transplanted to lateral cistern.Subarachnoid hemorrhage(SAH) group didn' t transplant stem cell.Stem cell culture media group injected DMEM media as DMEM group.Stem cell transplantation group injected 30μl Bone Marrow mesenchymal stem cell suspension,so called BMSCs group.Neurofunctional score and learning memory expression were detected with morris mazer and Neurofunctional Score Scale in each group.Results After transplantation for 7 d,functional score of Control,SAH,DMEM and Stem cell group were 3.95 ±2.51,7.20 ± 1.03,7.23 ± 1.79 and 5.81 ± 1.11 respectively.Compared with others groups,Stem cell group score was significantly decrease(P=0.017).After transplanting stem cell for 14 d,the mean spanning plate time in Control group,SAH group,DMEM group and Stem cell group were 7.38 ± 1.73,4.52 ± 0.90,5.11 ± 1.93 and 7.32 ± 2.16 respectively,SAH and DMEM group vs other 2 groups,there were clearly statistically differences (P =0.009),while between control group and stem cell group,there were no statistically differences (P =0.14).Conclusion SAH rat transplant stem cell can improve sense,motor and learning expression in certain level.