中华行为医学与脑科学杂志
中華行為醫學與腦科學雜誌
중화행위의학여뇌과학잡지
CHINESE JOURNAL OF BEHAVIORAL MEDICINE AND BRAIN SCIENCE
2013年
6期
503-506
,共4页
陈怡环%吴迪%陈冠婕%彭正午%王化宁%王莹%谭庆荣
陳怡環%吳迪%陳冠婕%彭正午%王化寧%王瑩%譚慶榮
진이배%오적%진관첩%팽정오%왕화저%왕형%담경영
迷迭香酸%创伤后应激障碍%白介素-1β%白介素-6%大鼠
迷迭香痠%創傷後應激障礙%白介素-1β%白介素-6%大鼠
미질향산%창상후응격장애%백개소-1β%백개소-6%대서
Rosmarinic acid%Posttraumatic stress disorder%Interlukin-1β%Interlukin-6%Rat
目的 探讨迷迭香酸(rosmarinic acid,RA)早期干预对增强型单次延长应激(enhanced single prolonged stress,ESPS)模型大鼠行为和海马白介素-1β (IL-1β)、白介素-6(IL-6)水平的影响.方法 将48只SD大鼠采用随机数字表法分为6组:对照组(Control)、RA低剂量组(Control+RA (L)),RA高剂量组(Control+RA (H)),模型组(ESPS),模型+RA低剂量组(ESPS+RA(L))和模型+RA高剂量组(ESPS+RA(H)),每组8只.采用旷场实验和高架十字迷宫检测大鼠行为表现,并且在行为实验完成后处死大鼠,用酶联免疫吸附法(enzyme linked immunosorbent assay,ELISA)测定大鼠海马中IL-1β和IL-6的水平.结果 (1)旷场实验:ESPS组大鼠进入中央区次数[(7.63±5.32)次]和中央区的探索时间百分比[(14.88±7.35)％]均显著低于Control组[分别为(18.13±10.15)次,(26.68±10.06)％]和ESPS+RA (H)组[分别为(16.88±8.81)次,(25.08±8.52)％],均差异有统计学意义(均P＜0.05).而Control+RA(L)、Control+RA(H)组与Control组之间,以及ESPS组和ESPS+RA(L)之间均差异无统计学意义(均P＞0.05).(2)高架十字迷宫实验:与Control组大鼠进入开臂次数百分比(37.38±8.24)％和开臂停留时间百分比(17.63±4.74)％相比,ESPS组有显著降低[分别为(21.92±6.82)％,(10.01±3.82)％],均差异有统计学意义(均P＜0.05)；与ESPS组比较,ESPS+RA(H)组大鼠进入开臂次数百分比和开臂停留时间百分比[分别为(33.72±9.49)％,(16.99±4.28)％]显著增加,差异有统计学意义(P＜0.05),而ESPS组和ESPS+RA(L)之间及Control+RA(L)、Control+RA(H)组与Control组之间均差异无统计学意义(均P＞0.05).(3)ELISA结果显示:ESPS组大鼠海马中IL-1β和IL-6的含量[(4.91±0.77)pg/mg,(38.31±9.44) pg/mg]高于Control组[(3.14 ±0.96) pg/mg,(27.15±8.03) pg/mg]和ESPS+RA (H)组[(3.48±0.86) pg/mg,(28.55±9.07) pg/mg],均P＜0.05.而Control+RA (L)、Control+RA (H)组与Control组之间,以及ESPS组和ESPS+RA(L)之间均差异无统计学意义(均P＞0.05).(4)相关分析结果显示:大鼠在旷场实验中进入中心区次数、中央区的探索时间百分比及高架十字迷宫实验中大鼠进入开臂的次数百分比与海马IL-1β的水平呈负相关(分别为:r=-0.435,r=-0.316,r=-0.311;均P＜0.05),但未发现大鼠海马IL-6的水平与大鼠的行为学结果存在关联.结论 迷迭香酸能改善PTSD动物焦虑样行为,而且这种作用可能是通过降低ESPS模型大鼠海马中IL-1β、IL-6的水平实现的.
目的 探討迷迭香痠(rosmarinic acid,RA)早期榦預對增彊型單次延長應激(enhanced single prolonged stress,ESPS)模型大鼠行為和海馬白介素-1β (IL-1β)、白介素-6(IL-6)水平的影響.方法 將48隻SD大鼠採用隨機數字錶法分為6組:對照組(Control)、RA低劑量組(Control+RA (L)),RA高劑量組(Control+RA (H)),模型組(ESPS),模型+RA低劑量組(ESPS+RA(L))和模型+RA高劑量組(ESPS+RA(H)),每組8隻.採用曠場實驗和高架十字迷宮檢測大鼠行為錶現,併且在行為實驗完成後處死大鼠,用酶聯免疫吸附法(enzyme linked immunosorbent assay,ELISA)測定大鼠海馬中IL-1β和IL-6的水平.結果 (1)曠場實驗:ESPS組大鼠進入中央區次數[(7.63±5.32)次]和中央區的探索時間百分比[(14.88±7.35)％]均顯著低于Control組[分彆為(18.13±10.15)次,(26.68±10.06)％]和ESPS+RA (H)組[分彆為(16.88±8.81)次,(25.08±8.52)％],均差異有統計學意義(均P＜0.05).而Control+RA(L)、Control+RA(H)組與Control組之間,以及ESPS組和ESPS+RA(L)之間均差異無統計學意義(均P＞0.05).(2)高架十字迷宮實驗:與Control組大鼠進入開臂次數百分比(37.38±8.24)％和開臂停留時間百分比(17.63±4.74)％相比,ESPS組有顯著降低[分彆為(21.92±6.82)％,(10.01±3.82)％],均差異有統計學意義(均P＜0.05)；與ESPS組比較,ESPS+RA(H)組大鼠進入開臂次數百分比和開臂停留時間百分比[分彆為(33.72±9.49)％,(16.99±4.28)％]顯著增加,差異有統計學意義(P＜0.05),而ESPS組和ESPS+RA(L)之間及Control+RA(L)、Control+RA(H)組與Control組之間均差異無統計學意義(均P＞0.05).(3)ELISA結果顯示:ESPS組大鼠海馬中IL-1β和IL-6的含量[(4.91±0.77)pg/mg,(38.31±9.44) pg/mg]高于Control組[(3.14 ±0.96) pg/mg,(27.15±8.03) pg/mg]和ESPS+RA (H)組[(3.48±0.86) pg/mg,(28.55±9.07) pg/mg],均P＜0.05.而Control+RA (L)、Control+RA (H)組與Control組之間,以及ESPS組和ESPS+RA(L)之間均差異無統計學意義(均P＞0.05).(4)相關分析結果顯示:大鼠在曠場實驗中進入中心區次數、中央區的探索時間百分比及高架十字迷宮實驗中大鼠進入開臂的次數百分比與海馬IL-1β的水平呈負相關(分彆為:r=-0.435,r=-0.316,r=-0.311;均P＜0.05),但未髮現大鼠海馬IL-6的水平與大鼠的行為學結果存在關聯.結論 迷迭香痠能改善PTSD動物焦慮樣行為,而且這種作用可能是通過降低ESPS模型大鼠海馬中IL-1β、IL-6的水平實現的.
목적 탐토미질향산(rosmarinic acid,RA)조기간예대증강형단차연장응격(enhanced single prolonged stress,ESPS)모형대서행위화해마백개소-1β (IL-1β)、백개소-6(IL-6)수평적영향.방법 장48지SD대서채용수궤수자표법분위6조:대조조(Control)、RA저제량조(Control+RA (L)),RA고제량조(Control+RA (H)),모형조(ESPS),모형+RA저제량조(ESPS+RA(L))화모형+RA고제량조(ESPS+RA(H)),매조8지.채용광장실험화고가십자미궁검측대서행위표현,병차재행위실험완성후처사대서,용매련면역흡부법(enzyme linked immunosorbent assay,ELISA)측정대서해마중IL-1β화IL-6적수평.결과 (1)광장실험:ESPS조대서진입중앙구차수[(7.63±5.32)차]화중앙구적탐색시간백분비[(14.88±7.35)％]균현저저우Control조[분별위(18.13±10.15)차,(26.68±10.06)％]화ESPS+RA (H)조[분별위(16.88±8.81)차,(25.08±8.52)％],균차이유통계학의의(균P＜0.05).이Control+RA(L)、Control+RA(H)조여Control조지간,이급ESPS조화ESPS+RA(L)지간균차이무통계학의의(균P＞0.05).(2)고가십자미궁실험:여Control조대서진입개비차수백분비(37.38±8.24)％화개비정류시간백분비(17.63±4.74)％상비,ESPS조유현저강저[분별위(21.92±6.82)％,(10.01±3.82)％],균차이유통계학의의(균P＜0.05)；여ESPS조비교,ESPS+RA(H)조대서진입개비차수백분비화개비정류시간백분비[분별위(33.72±9.49)％,(16.99±4.28)％]현저증가,차이유통계학의의(P＜0.05),이ESPS조화ESPS+RA(L)지간급Control+RA(L)、Control+RA(H)조여Control조지간균차이무통계학의의(균P＞0.05).(3)ELISA결과현시:ESPS조대서해마중IL-1β화IL-6적함량[(4.91±0.77)pg/mg,(38.31±9.44) pg/mg]고우Control조[(3.14 ±0.96) pg/mg,(27.15±8.03) pg/mg]화ESPS+RA (H)조[(3.48±0.86) pg/mg,(28.55±9.07) pg/mg],균P＜0.05.이Control+RA (L)、Control+RA (H)조여Control조지간,이급ESPS조화ESPS+RA(L)지간균차이무통계학의의(균P＞0.05).(4)상관분석결과현시:대서재광장실험중진입중심구차수、중앙구적탐색시간백분비급고가십자미궁실험중대서진입개비적차수백분비여해마IL-1β적수평정부상관(분별위:r=-0.435,r=-0.316,r=-0.311;균P＜0.05),단미발현대서해마IL-6적수평여대서적행위학결과존재관련.결론 미질향산능개선PTSD동물초필양행위,이차저충작용가능시통과강저ESPS모형대서해마중IL-1β、IL-6적수평실현적.
Objective To investigate the effect of rosmarinic acid on the behavioral changes in enhanced single prolonged stress (ESPS) model rats and the levels of interlukin-1β (IL-1β) and interlukin-6 (IL-6) in the hippocampus.Methods 48 adult male Sprague Dawley rats were randomly divided into six groups (n =8):Control group,Control + RA (L) group,Control + RA (H) group,ESPS group,ESPS + RA (L) group and ESPS + RA (H) group.Behavioral changes of these rats were analyzed by open field test and elevated plus-maze.The levels of IL-1 β and IL-6 were measured by enzyme linked immunosorbent assay (ELISA).Results (1) Open field test showed that the number of central region entering and the fraction of time exploring in center of ESPS group were significantly reduced than that of Control group ((18.13 ± 10.15) times,(26.68 ± 10.06) ％) and ESPS + RA (H) group ((16.88 ± 8.81) times,(25.08 ± 8.52) ％) (P ＜ 0.05).And it showed no significant difference among Control + RA(L) group,Control + RA(H) group and Control group.Meanwhile,there was also no statistic difference between ESPS group and ESPS + RA(L) group.(2) Elevated plus-maze test showed that percentages of open arm entries and fraction of time exploring in open arm in reference to total number of entries into all arms and total time spent on all arms in ESPS group were significantly reduced than that of Control group((37.38 ± 8.24)％,(17.63 ±4.74)％) and ESPS + RA(H) group((33.72 ±9.49)％,(16.99 ±4.28)％) (P ＜ 0.05),but there was no significant difference between that of ESPS group and ESPS + RA(L) group(P＞0.05).It also showed no significant difference among Control + RA (L) group,Control + RA (H) group and Control group.(3) Compared with ESPS group,RA(10mg/kg) reduced the levels of IL-1β and IL-6 in the hippocampus,but RA(5mg/kg) did not have this effect.(4) Correlation analysis results showed the level of IL-1β in the hippocampus was negatively related with the ameliorated PTSD-like behaviors of ESPS exposure rats.Conclusion RA can ameliorate PTSDlike behaviors of ESPS exposure rats,and this effect may be carried out by down-regulating the levels of IL-1β and IL-6 in the hippocampus,especially the IL-1β.
