目的 比较青年与老龄昆明小鼠焦虑动物模型结构维度的不同.方法 青年与老龄雄性昆明小鼠依次进行旷场实验(open field test,OFT)、高架十字迷宫实验(elevated plus maze,EPM)和明暗箱实验(light/dark box,LDB),实验间隔1周,摄像系统记录5 min的行为变化,实验参数包括:OFT中央区停留时间百分率、中央区水平运动百分率(Ccross％)、总水平运动(Cross)、总垂直运动(Rear),EPM开臂区停留时间百分率(Otime％)、开臂区进入次数百分率(Oentries％)、两臂总进入次数(Entries),LDB明箱区停留时间百分率(Ltime％)、明箱区水平运动百分率(Lcross％)、明箱区垂直运动百分率(Lrear％)、穿梭次数(Transition)、总水平运动(Cross)、总垂直运动(Rear).结果 老龄组与青年组相比,OFT焦虑因子参数(Ctime％,t=3.12,P＜0.01；Ccross％,t=2.37,P＜0.01)、EPM焦虑因子参数(Otime％,t=1.31,P＜0.05；Oentries％,t=1.57,P＜0.05)、LDB焦虑因子参数(Ltime％,t=4.15,P＜0.01；Lcross％,t=3.28,P＜0.01；Lrear％,t=2.73,P＜0.01)均升高,而OFT运动探索因子参数(Cross,t=2.05,P＜0.01)、EPM运动探索因子参数(Entries,t=2.09,P＜0.05)、LDB运动探索因子参数(Transition,t=2.51,P＜0.01；Cross,t=3.02,P＜0.01)均降低.结论 青年与老龄昆明小鼠焦虑动物模型具有相似的结构维度,但是随着年龄增长,焦虑情绪增加,运动探索活性降低.
目的 比較青年與老齡昆明小鼠焦慮動物模型結構維度的不同.方法 青年與老齡雄性昆明小鼠依次進行曠場實驗(open field test,OFT)、高架十字迷宮實驗(elevated plus maze,EPM)和明暗箱實驗(light/dark box,LDB),實驗間隔1週,攝像繫統記錄5 min的行為變化,實驗參數包括:OFT中央區停留時間百分率、中央區水平運動百分率(Ccross％)、總水平運動(Cross)、總垂直運動(Rear),EPM開臂區停留時間百分率(Otime％)、開臂區進入次數百分率(Oentries％)、兩臂總進入次數(Entries),LDB明箱區停留時間百分率(Ltime％)、明箱區水平運動百分率(Lcross％)、明箱區垂直運動百分率(Lrear％)、穿梭次數(Transition)、總水平運動(Cross)、總垂直運動(Rear).結果 老齡組與青年組相比,OFT焦慮因子參數(Ctime％,t=3.12,P＜0.01；Ccross％,t=2.37,P＜0.01)、EPM焦慮因子參數(Otime％,t=1.31,P＜0.05；Oentries％,t=1.57,P＜0.05)、LDB焦慮因子參數(Ltime％,t=4.15,P＜0.01；Lcross％,t=3.28,P＜0.01；Lrear％,t=2.73,P＜0.01)均升高,而OFT運動探索因子參數(Cross,t=2.05,P＜0.01)、EPM運動探索因子參數(Entries,t=2.09,P＜0.05)、LDB運動探索因子參數(Transition,t=2.51,P＜0.01；Cross,t=3.02,P＜0.01)均降低.結論 青年與老齡昆明小鼠焦慮動物模型具有相似的結構維度,但是隨著年齡增長,焦慮情緒增加,運動探索活性降低.
목적 비교청년여노령곤명소서초필동물모형결구유도적불동.방법 청년여노령웅성곤명소서의차진행광장실험(open field test,OFT)、고가십자미궁실험(elevated plus maze,EPM)화명암상실험(light/dark box,LDB),실험간격1주,섭상계통기록5 min적행위변화,실험삼수포괄:OFT중앙구정류시간백분솔、중앙구수평운동백분솔(Ccross％)、총수평운동(Cross)、총수직운동(Rear),EPM개비구정류시간백분솔(Otime％)、개비구진입차수백분솔(Oentries％)、량비총진입차수(Entries),LDB명상구정류시간백분솔(Ltime％)、명상구수평운동백분솔(Lcross％)、명상구수직운동백분솔(Lrear％)、천사차수(Transition)、총수평운동(Cross)、총수직운동(Rear).결과 노령조여청년조상비,OFT초필인자삼수(Ctime％,t=3.12,P＜0.01；Ccross％,t=2.37,P＜0.01)、EPM초필인자삼수(Otime％,t=1.31,P＜0.05；Oentries％,t=1.57,P＜0.05)、LDB초필인자삼수(Ltime％,t=4.15,P＜0.01；Lcross％,t=3.28,P＜0.01；Lrear％,t=2.73,P＜0.01)균승고,이OFT운동탐색인자삼수(Cross,t=2.05,P＜0.01)、EPM운동탐색인자삼수(Entries,t=2.09,P＜0.05)、LDB운동탐색인자삼수(Transition,t=2.51,P＜0.01；Cross,t=3.02,P＜0.01)균강저.결론 청년여노령곤명소서초필동물모형구유상사적결구유도,단시수착년령증장,초필정서증가,운동탐색활성강저.
Objective To explore the effect of different ages on behavioral dimensions of anxiety animal models in Kunming mice.Methods Young/Old,male,Kunming mice were recorded twice,for five minutes,with a one-week inter-trial interval in open field test (OFT),elevated plus maze (EPM) and light/dark box (LDB).The following parameters were evaluated:percentage of time spent in the OFT central area (Ctime％),percentage of number of squares visited in the OFT central area (Ccross％),the total number of horizontal squares in OFT (Cross),the total number of rears in OFT (Rear),percentage of time spent in the EPM open arms (Otime％),percentage of number of entries into the EPM open arms (Oentries％),the total number of entries into the EPM open and closed arms (Entries),percentage of time exploring in the LDB light area (Ltime％),percentage of number of squares crossing in the LDB light area (Lcross％),percentage of rears in the LDB light area (Lrear％),the total number of transition in LDB (Transition),the total number of horizontal squares in LDB (Cross) and the total number of rears in LDB (Rear).Results OFT-Ctime％,OFT-Ccross％,EPM-Otime％,EPM-Oentries％,LDB-Ltime％,LDB-Lcross％ and LDB-Lrear％ had significant loadings on anxiety factor; OFTCross,EPM-Entries,LDB-Transition and LDB-Cross loaded on locomotion-exploration factor.Old mice had higher anxiety emotion (Ctime％,t =3.12,P＜ 0.01; Ccross％,t =2.37,P＜ 0.01; Otime％,t =1.31,P＜ 0.05; Oentries％,t=1.57,P＜0.05; Ltime％,t=4.15,P＜0.01 ; Lcross％,t=3.28,P＜0.01 ; Lrear％,t=2.73,P＜0.01) and lower locomotion and exploratory activity (Cross,t =2.05,P ＜ 0.01 ; Entries,t =2.09,P ＜ 0.05 ;Transition,t=2.51,P＜0.01; Cross,t=3.02,P＜0.01),compared with Young.Conclusion Ages have no effect on structural dimensions of anxiety animal models.With the growth of ages,old mice have higher anxiety emotion but lower locomotion and exploratory activity.
