CAJ | 학술논문

目的：探讨环吡酮胺乳膏应用高效液相色谱法进行药物含量与体外透皮释放量测定的效果。方法检测波长305 nm；流动相：乙腈-0.1%乙二胺四醋酸二钠溶液(70：30)；Kromasil C18色谱柱；应用单室Franz扩散池、大鼠腹皮进行透皮释放实验,对比市面乳膏与自制乳膏的透皮结果。结果环吡酮胺范围介于20~180μg/mL间,平均回收率99.8%,RSD为0.70%,线性关系较好(r=0.9997),相关因素为0.9673,市面乳膏与自制乳膏透皮结果差异无统计学意义(P>0.05)。结论采用高效液相色谱法测定环吡酮胺乳膏不会受皮肤成分与软膏基质影响,且操作简便,准确率高,测定效果理想。
목적：탐토배필동알유고응용고효액상색보법진행약물함량여체외투피석방량측정적효과。방법검측파장305 nm；류동상：을정-0.1%을이알사작산이납용액(70：30)；Kromasil C18색보주；응용단실Franz확산지、대서복피진행투피석방실험,대비시면유고여자제유고적투피결과。결과배필동알범위개우20~180μg/mL간,평균회수솔99.8%,RSD위0.70%,선성관계교호(r=0.9997),상관인소위0.9673,시면유고여자제유고투피결과차이무통계학의의(P>0.05)。결론채용고효액상색보법측정배필동알유고불회수피부성분여연고기질영향,차조작간편,준학솔고,측정효과이상。
Objective To determinate content of drugs in ciclopirox olamine cream by high performance liquid chromatography method(HPLC) and its in vitro transdermal release.MethodsDetection wavelength of 305 nm; mobile phase: acetonitrile -0.1% four acetic acid disodium ethylenediamine solution(70-30); Kromasil C18 chromatographic column; application of single chamber Franz diffusion pool, big abdomen skin transdermal release experiments, contrasted the transdermal cream with homemade cream market results.ResultsCiclopirox olamine cream range between 20~180μg/ml, the average recovery was 99.8%, RSD was 0.70%, had good linear relationship (r=0.9997), and related factors 0.9673, cream market and the results of the homemade cream transdermal no statistical significance(P>0.05).ConclusionBy high performance liquid chromatography determination of ciclopirox olamine cream won’t be affected by the composition of the skin with cream base with easy operation, high accuracy, ideal measurement effect.